Restriction map for
pF1KB8313
(Linear) (Six-base) MAPSORT of: KIBB8313.seq Check: 7399 from: 1 to: 1104
KIBB8313.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 557 1104
Size: 557 547
AccI GT'mk_AC
Cuts at: 0 854 1104
Size: 854 250
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1104
Size: 154 950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 719 776 1104
Size: 719 57 328
Fragments arranged by size:
719 328 57
AlwNI CAG_nnn'CTG
Cuts at: 0 198 801 895 942 985 1104
Size: 198 603 94 47 43 119
Fragments arranged by size:
603 198 119 94 47 43
AvaI C'yCGr_G
Cuts at: 0 157 251 471 1104
Size: 157 94 220 633
Fragments arranged by size:
633 220 157 94
BanII G_rGCy'C
Cuts at: 0 377 533 1104
Size: 377 156 571
Fragments arranged by size:
571 377 156
BglI GCCn_nnn'nGGC
Cuts at: 0 62 1104
Size: 62 1042
BglII A'GATC_T
Cuts at: 0 1037 1104
Size: 1037 67
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 552 1104
Size: 552 552
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 1104
Size: 447 657
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 1104
Size: 364 740
BsaI GGTCTCn'nnnn_
Cuts at: 0 1003 1104
Size: 1003 101
BsaWI w'CCGG_w
Cuts at: 0 440 1104
Size: 440 664
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 367 595 673 676 679 1104
Size: 367 228 78 3 3 425
Fragments arranged by size:
425 367 228 78 3 3
BseYI C'CCAG_C
Cuts at: 0 333 522 867 1043 1104
Size: 333 189 345 176 61
Fragments arranged by size:
345 333 189 176 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 1104
Size: 225 879
BsiEI CG_ry'CG
Cuts at: 0 57 1104
Size: 57 1047
BsiHKAI G_wGCw'C
Cuts at: 0 377 463 488 533 1104
Size: 377 86 25 45 571
Fragments arranged by size:
571 377 86 45 25
Bsp1286I G_dGCh'C
Cuts at: 0 377 463 488 533 1104
Size: 377 86 25 45 571
Fragments arranged by size:
571 377 86 45 25
BspEI T'CCGG_A
Cuts at: 0 440 1104
Size: 440 664
BspHI T'CATG_A
Cuts at: 0 1064 1104
Size: 1064 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 557 1104
Size: 557 547
BssSI C'ACGA_G
Cuts at: 0 340 1104
Size: 340 764
BstYI r'GATC_y
Cuts at: 0 230 314 1037 1104
Size: 230 84 723 67
Fragments arranged by size:
723 230 84 67
BtgI C'CryG_G
Cuts at: 0 57 580 1004 1104
Size: 57 523 424 100
Fragments arranged by size:
523 424 100 57
BtsI GCAGTG_nn'
Cuts at: 0 265 637 1104
Size: 265 372 467
Fragments arranged by size:
467 372 265
EaeI y'GGCC_r
Cuts at: 0 54 1104
Size: 54 1050
EagI C'GGCC_G
Cuts at: 0 54 1104
Size: 54 1050
EarI CTCTTCn'nnn_
Cuts at: 0 538 1104
Size: 538 566
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 691 1104
Size: 691 413
EcoICRI GAG'CTC
Cuts at: 0 375 531 1104
Size: 375 156 573
Fragments arranged by size:
573 375 156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 447 1104
Size: 154 293 657
Fragments arranged by size:
657 293 154
EcoO109I rG'GnC_Cy
Cuts at: 0 138 297 892 1104
Size: 138 159 595 212
Fragments arranged by size:
595 212 159 138
HaeII r_GCGC'y
Cuts at: 0 888 1104
Size: 888 216
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 58 90 1104
Size: 58 32 1014
Fragments arranged by size:
1014 58 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 842 1104
Size: 842 262
MspA1I CmG'CkG
Cuts at: 0 693 1104
Size: 693 411
NspI r_CATG'y
Cuts at: 0 597 1104
Size: 597 507
PpuMI rG'GwC_Cy
Cuts at: 0 138 297 1104
Size: 138 159 807
Fragments arranged by size:
807 159 138
PspXI vC'TCGA_Gb
Cuts at: 0 157 471 1104
Size: 157 314 633
Fragments arranged by size:
633 314 157
PstI C_TGCA'G
Cuts at: 0 272 554 698 1104
Size: 272 282 144 406
Fragments arranged by size:
406 282 272 144
PvuII CAG'CTG
Cuts at: 0 693 1104
Size: 693 411
SacI G_AGCT'C
Cuts at: 0 377 533 1104
Size: 377 156 571
Fragments arranged by size:
571 377 156
SanDI GG'GwC_CC
Cuts at: 0 297 1104
Size: 297 807
SapI GCTCTTCn'nnn_
Cuts at: 0 538 1104
Size: 538 566
ScaI AGT'ACT
Cuts at: 0 736 1104
Size: 736 368
SfcI C'TryA_G
Cuts at: 0 268 550 694 951 1104
Size: 268 282 144 257 153
Fragments arranged by size:
282 268 257 153 144
SmaI CCC'GGG
Cuts at: 0 253 1104
Size: 253 851
SmlI C'TyrA_G
Cuts at: 0 157 379 471 1104
Size: 157 222 92 633
Fragments arranged by size:
633 222 157 92
StuI AGG'CCT
Cuts at: 0 517 1104
Size: 517 587
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 446 1104
Size: 446 658
TatI w'GTAC_w
Cuts at: 0 734 1104
Size: 734 370
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 187 219 1104
Size: 187 32 885
Fragments arranged by size:
885 187 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 111 1104
Size: 111 993
XhoI C'TCGA_G
Cuts at: 0 157 471 1104
Size: 157 314 633
Fragments arranged by size:
633 314 157
XmaI C'CCGG_G
Cuts at: 0 251 1104
Size: 251 853
Enzymes that do cut:
AarI AccI AcuI AlfI AlwNI AvaI BanII BglI
BglII BplI BpmI BpuEI BsaI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BssSI
BstYI BtgI BtsI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I HaeII Hin4I MmeI MspA1I NspI PpuMI
PspXI PstI PvuII SacI SanDI SapI ScaI SfcI
SmaI SmlI StuI TaqII TatI TstI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BlpI Bme1580I BmgBI BmrI BmtI
Bpu10I BsaAI BsaBI BsaHI BsaXI BsiWI BsmI BsmBI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII
SacII SalI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StyI SwaI Tth111I
XbaI XmnI ZraI