Restriction map for
pF1KB8315
(Linear) (Six-base) MAPSORT of: KIBB8315.seq Check: 5897 from: 1 to: 762
KIBB8315.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 762
Size: 449 313
AlwNI CAG_nnn'CTG
Cuts at: 0 379 687 762
Size: 379 308 75
Fragments arranged by size:
379 308 75
ApoI r'AATT_y
Cuts at: 0 192 762
Size: 192 570
AvaI C'yCGr_G
Cuts at: 0 322 762
Size: 322 440
BanII G_rGCy'C
Cuts at: 0 323 526 762
Size: 323 203 236
Fragments arranged by size:
323 236 203
BbsI GAAGACnn'nnnn_
Cuts at: 0 413 762
Size: 413 349
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2 36 762
Size: 2 34 726
Fragments arranged by size:
726 34 2
BciVI GTATCCnnnnn_n'
Cuts at: 0 18 762
Size: 18 744
BglII A'GATC_T
Cuts at: 0 15 110 762
Size: 15 95 652
Fragments arranged by size:
652 95 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 501 762
Size: 501 261
BsaI GGTCTCn'nnnn_
Cuts at: 0 118 762
Size: 118 644
BsaBI GATnn'nnATC
Cuts at: 0 410 762
Size: 410 352
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 310 340 762
Size: 310 30 422
Fragments arranged by size:
422 310 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 159 448 546 567 762
Size: 159 289 98 21 195
Fragments arranged by size:
289 195 159 98 21
BseYI C'CCAG_C
Cuts at: 0 151 643 648 762
Size: 151 492 5 114
Fragments arranged by size:
492 151 114 5
BsiEI CG_ry'CG
Cuts at: 0 468 762
Size: 468 294
BsiHKAI G_wGCw'C
Cuts at: 0 526 636 762
Size: 526 110 126
Fragments arranged by size:
526 126 110
Bsp1286I G_dGCh'C
Cuts at: 0 323 526 636 762
Size: 323 203 110 126
Fragments arranged by size:
323 203 126 110
BsrBI CCG'CTC
Cuts at: 0 672 762
Size: 672 90
BsrFI r'CCGG_y
Cuts at: 0 50 331 762
Size: 50 281 431
Fragments arranged by size:
431 281 50
BstYI r'GATC_y
Cuts at: 0 15 110 762
Size: 15 95 652
Fragments arranged by size:
652 95 15
DraIII CAC_nnn'GTG
Cuts at: 0 399 762
Size: 399 363
EaeI y'GGCC_r
Cuts at: 0 465 762
Size: 465 297
EagI C'GGCC_G
Cuts at: 0 465 762
Size: 465 297
EarI CTCTTCn'nnn_
Cuts at: 0 281 425 597 721 762
Size: 281 144 172 124 41
Fragments arranged by size:
281 172 144 124 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 150 340 762
Size: 150 190 422
Fragments arranged by size:
422 190 150
EcoICRI GAG'CTC
Cuts at: 0 524 762
Size: 524 238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 501 762
Size: 449 52 261
Fragments arranged by size:
449 261 52
EcoNI CCTnn'n_nnAGG
Cuts at: 0 77 762
Size: 77 685
EcoO109I rG'GnC_Cy
Cuts at: 0 666 762
Size: 666 96
EcoRI G'AATT_C
Cuts at: 0 192 762
Size: 192 570
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 129 762
Size: 129 633
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 403 435 762
Size: 310 32 61 32 327
Fragments arranged by size:
327 310 61 32 32
MspA1I CmG'CkG
Cuts at: 0 684 762
Size: 684 78
NaeI GCC'GGC
Cuts at: 0 333 762
Size: 333 429
NgoMIV G'CCGG_C
Cuts at: 0 331 762
Size: 331 431
PfoI T'CCnGG_A
Cuts at: 0 517 762
Size: 517 245
PpuMI rG'GwC_Cy
Cuts at: 0 666 762
Size: 666 96
PvuII CAG'CTG
Cuts at: 0 684 762
Size: 684 78
SacI G_AGCT'C
Cuts at: 0 526 762
Size: 526 236
SexAI A'CCwGG_T
Cuts at: 0 395 762
Size: 395 367
SfcI C'TryA_G
Cuts at: 0 74 762
Size: 74 688
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 525 762
Size: 525 237
XmnI GAAnn'nnTTC
Cuts at: 0 112 762
Size: 112 650
Enzymes that do cut:
AcuI AlwNI ApoI AvaI BanII BbsI BcgI BciVI
BglII BpmI BsaI BsaBI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BsrBI BsrFI BstYI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
Hin4I MspA1I NaeI NgoMIV PfoI PpuMI PvuII SacI
SexAI SfcI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaAI BsaHI BsaWI BsgI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI NarI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmaI ZraI