Restriction map for
pF1KB8322
(Linear) (Six-base) MAPSORT of: KIBB8322.seq Check: 8781 from: 1 to: 2004
KIBB8322.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AccI GT'mk_AC
Cuts at: 0 1423 2004
Size: 1423 581
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 1341 2004
Size: 21 1320 663
Fragments arranged by size:
1320 663 21
AfeI AGC'GCT
Cuts at: 0 556 661 679 1705 2004
Size: 556 105 18 1026 299
Fragments arranged by size:
1026 556 299 105 18
AflIII A'CryG_T
Cuts at: 0 524 1169 1568 1818 2004
Size: 524 645 399 250 186
Fragments arranged by size:
645 524 399 250 186
AhdI GACnn_n'nnGTC
Cuts at: 0 462 2004
Size: 462 1542
AleI CACnn'nnGTG
Cuts at: 0 89 1583 1669 2004
Size: 89 1494 86 335
Fragments arranged by size:
1494 335 89 86
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 64 153 2004
Size: 64 89 1851
Fragments arranged by size:
1851 89 64
AlwNI CAG_nnn'CTG
Cuts at: 0 822 1374 1458 1610 2004
Size: 822 552 84 152 394
Fragments arranged by size:
822 552 394 152 84
ApaLI G'TGCA_C
Cuts at: 0 345 846 1518 1732 2004
Size: 345 501 672 214 272
Fragments arranged by size:
672 501 345 272 214
AvaI C'yCGr_G
Cuts at: 0 52 384 399 427 697 909 1006 2004
Size: 52 332 15 28 270 212 97 998
Fragments arranged by size:
998 332 270 212 97 52 28 15
BamHI G'GATC_C
Cuts at: 0 389 2004
Size: 389 1615
BanI G'GyrC_C
Cuts at: 0 874 931 1873 2004
Size: 874 57 942 131
Fragments arranged by size:
942 874 131 57
BanII G_rGCy'C
Cuts at: 0 890 1723 2004
Size: 890 833 281
Fragments arranged by size:
890 833 281
BbsI GAAGACnn'nnnn_
Cuts at: 0 635 860 2004
Size: 635 225 1144
Fragments arranged by size:
1144 635 225
BbvCI CC'TCA_GC
Cuts at: 0 1031 2004
Size: 1031 973
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 336 370 997 1031 2004
Size: 336 34 627 34 973
Fragments arranged by size:
973 627 336 34 34
BfrBI ATG'CAT
Cuts at: 0 1989 2004
Size: 1989 15
BglI GCCn_nnn'nGGC
Cuts at: 0 1892 2004
Size: 1892 112
Bme1580I G_kGCm'C
Cuts at: 0 349 424 850 877 1522 1736 1878 2004
Size: 349 75 426 27 645 214 142 126
Fragments arranged by size:
645 426 349 214 142 126 75 27
BmgBI CAC'GTC
Cuts at: 0 440 1172 2004
Size: 440 732 832
Fragments arranged by size:
832 732 440
BmrI ACTGGGnnnn_n'
Cuts at: 0 1386 2004
Size: 1386 618
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 960 1265 2004
Size: 960 305 739
Fragments arranged by size:
960 739 305
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1831 2004
Size: 1831 173
Bpu10I CC'TnA_GC
Cuts at: 0 1031 2004
Size: 1031 973
BsaI GGTCTCn'nnnn_
Cuts at: 0 49 279 694 2004
Size: 49 230 415 1310
Fragments arranged by size:
1310 415 230 49
BsaAI yAC'GTr
Cuts at: 0 1821 2004
Size: 1821 183
BsaHI Gr'CG_yC
Cuts at: 0 914 2004
Size: 914 1090
BsaWI w'CCGG_w
Cuts at: 0 1077 2004
Size: 1077 927
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 514 544 943 973 1915 1945 2004
Size: 514 30 399 30 942 30 59
Fragments arranged by size:
942 514 399 59 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 856 912 2004
Size: 856 56 1092
Fragments arranged by size:
1092 856 56
BseYI C'CCAG_C
Cuts at: 0 634 1199 1322 1409 1941 2004
Size: 634 565 123 87 532 63
Fragments arranged by size:
634 565 532 123 87 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 835 1418 1813 1890 2004
Size: 244 591 583 395 77 114
Fragments arranged by size:
591 583 395 244 114 77
BsiEI CG_ry'CG
Cuts at: 0 308 2004
Size: 308 1696
BsiHKAI G_wGCw'C
Cuts at: 0 53 349 532 850 1522 1723 1736 2004
Size: 53 296 183 318 672 201 13 268
Fragments arranged by size:
672 318 296 268 201 183 53 13
BsmI GAATG_Cn'
Cuts at: 0 11 2004
Size: 11 1993
Bsp1286I G_dGCh'C
Cuts at: 0 53 349 424 532 850 877 890 1522
Size: 53 296 75 108 318 27 13 632
Cuts at: 1522 1723 1736 1878 2004
Size: 201 13 142 126
Fragments arranged by size:
632 318 296 201 142 126 108 75
53 27 13 13
BspEI T'CCGG_A
Cuts at: 0 1077 2004
Size: 1077 927
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 482 1263 1310 2004
Size: 482 781 47 694
Fragments arranged by size:
781 694 482 47
BsrDI GCAATG_nn'
Cuts at: 0 1095 1186 1848 2004
Size: 1095 91 662 156
Fragments arranged by size:
1095 662 156 91
BsrFI r'CCGG_y
Cuts at: 0 151 2004
Size: 151 1853
BsrGI T'GTAC_A
Cuts at: 0 254 2004
Size: 254 1750
BssHII G'CGCG_C
Cuts at: 0 126 2004
Size: 126 1878
BssSI C'ACGA_G
Cuts at: 0 589 2004
Size: 589 1415
BstAPI GCAn_nnn'nTGC
Cuts at: 0 231 813 1188 1526 2004
Size: 231 582 375 338 478
Fragments arranged by size:
582 478 375 338 231
BstYI r'GATC_y
Cuts at: 0 203 296 389 506 818 2004
Size: 203 93 93 117 312 1186
Fragments arranged by size:
1186 312 203 117 93 93
Bsu36I CC'TnA_GG
Cuts at: 0 1240 2004
Size: 1240 764
BtgI C'CryG_G
Cuts at: 0 78 111 415 1328 2004
Size: 78 33 304 913 676
Fragments arranged by size:
913 676 304 78 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 389 2004
Size: 389 1615
BtsI GCAGTG_nn'
Cuts at: 0 581 708 1341 2004
Size: 581 127 633 663
Fragments arranged by size:
663 633 581 127
DraIII CAC_nnn'GTG
Cuts at: 0 498 1656 2004
Size: 498 1158 348
Fragments arranged by size:
1158 498 348
DrdI GACnn_nn'nnGTC
Cuts at: 0 913 1144 2004
Size: 913 231 860
Fragments arranged by size:
913 860 231
EaeI y'GGCC_r
Cuts at: 0 81 305 2004
Size: 81 224 1699
Fragments arranged by size:
1699 224 81
EagI C'GGCC_G
Cuts at: 0 305 2004
Size: 305 1699
EarI CTCTTCn'nnn_
Cuts at: 0 1318 1360 1711 2004
Size: 1318 42 351 293
Fragments arranged by size:
1318 351 293 42
EcoICRI GAG'CTC
Cuts at: 0 1721 2004
Size: 1721 283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 1341 1831 2004
Size: 21 1320 490 173
Fragments arranged by size:
1320 490 173 21
EcoO109I rG'GnC_Cy
Cuts at: 0 1847 2004
Size: 1847 157
FspI TGC'GCA
Cuts at: 0 106 736 2004
Size: 106 630 1268
Fragments arranged by size:
1268 630 106
FspAI rTGC'GCAy
Cuts at: 0 106 736 2004
Size: 106 630 1268
Fragments arranged by size:
1268 630 106
HaeII r_GCGC'y
Cuts at: 0 558 663 681 1450 1620 1707 2004
Size: 558 105 18 769 170 87 297
Fragments arranged by size:
769 558 297 170 105 87 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 193 225 448 480 2004
Size: 193 32 223 32 1524
Fragments arranged by size:
1524 223 193 32 32
HincII GTy'rAC
Cuts at: 0 1167 1424 2004
Size: 1167 257 580
Fragments arranged by size:
1167 580 257
HindIII A'AGCT_T
Cuts at: 0 900 2004
Size: 900 1104
MluI A'CGCG_T
Cuts at: 0 524 2004
Size: 524 1480
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 1160 2004
Size: 263 897 844
Fragments arranged by size:
897 844 263
MscI TGG'CCA
Cuts at: 0 83 2004
Size: 83 1921
MslI CAynn'nnrTG
Cuts at: 0 89 1583 1669 1683 1984 2004
Size: 89 1494 86 14 301 20
Fragments arranged by size:
1494 301 89 86 20 14
MspA1I CmG'CkG
Cuts at: 0 137 260 417 548 569 638 1030 1288
Size: 137 123 157 131 21 69 392 258
Cuts at: 1288 1330 1440 2004
Size: 42 110 564
Fragments arranged by size:
564 392 258 157 137 131 123 110
69 42 21
NaeI GCC'GGC
Cuts at: 0 153 2004
Size: 153 1851
NgoMIV G'CCGG_C
Cuts at: 0 151 2004
Size: 151 1853
NsiI A_TGCA'T
Cuts at: 0 1991 2004
Size: 1991 13
NspI r_CATG'y
Cuts at: 0 123 1572 1740 1993 2004
Size: 123 1449 168 253 11
Fragments arranged by size:
1449 253 168 123 11
PciI A'CATG_T
Cuts at: 0 1568 2004
Size: 1568 436
PflMI CCAn_nnn'nTGG
Cuts at: 0 624 2004
Size: 624 1380
PfoI T'CCnGG_A
Cuts at: 0 167 392 821 1808 2004
Size: 167 225 429 987 196
Fragments arranged by size:
987 429 225 196 167
PmlI CAC'GTG
Cuts at: 0 1821 2004
Size: 1821 183
PpuMI rG'GwC_Cy
Cuts at: 0 1847 2004
Size: 1847 157
PstI C_TGCA'G
Cuts at: 0 296 323 479 1020 1860 2004
Size: 296 27 156 541 840 144
Fragments arranged by size:
840 541 296 156 144 27
PvuII CAG'CTG
Cuts at: 0 638 1288 1440 2004
Size: 638 650 152 564
Fragments arranged by size:
650 638 564 152
SacI G_AGCT'C
Cuts at: 0 1723 2004
Size: 1723 281
SacII CC_GC'GG
Cuts at: 0 418 1331 2004
Size: 418 913 673
Fragments arranged by size:
913 673 418
SalI G'TCGA_C
Cuts at: 0 1422 2004
Size: 1422 582
SanDI GG'GwC_CC
Cuts at: 0 1847 2004
Size: 1847 157
SapI GCTCTTCn'nnn_
Cuts at: 0 1711 2004
Size: 1711 293
SbfI CC_TGCA'GG
Cuts at: 0 1860 2004
Size: 1860 144
SfcI C'TryA_G
Cuts at: 0 189 292 319 475 1016 1856 2004
Size: 189 103 27 156 541 840 148
Fragments arranged by size:
840 541 189 156 148 103 27
SmaI CCC'GGG
Cuts at: 0 386 2004
Size: 386 1618
SmlI C'TyrA_G
Cuts at: 0 52 2004
Size: 52 1952
SphI G_CATG'C
Cuts at: 0 123 2004
Size: 123 1881
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 500 1015 1152 1726 2004
Size: 19 481 515 137 574 278
Fragments arranged by size:
574 515 481 278 137 19
TatI w'GTAC_w
Cuts at: 0 254 2004
Size: 254 1750
Tth111I GACn'n_nGTC
Cuts at: 0 1404 2004
Size: 1404 600
XhoI C'TCGA_G
Cuts at: 0 52 2004
Size: 52 1952
XmaI C'CCGG_G
Cuts at: 0 384 2004
Size: 384 1620
Enzymes that do cut:
AccI AcuI AfeI AflIII AhdI AleI AlfI AlwNI
ApaLI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BfrBI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstYI Bsu36I BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI
EcoO109I FspI FspAI HaeII Hin4I HincII HindIII MluI
MmeI MscI MslI MspA1I NaeI NgoMIV NsiI NspI
PciI PflMI PfoI PmlI PpuMI PstI PvuII SacI
SacII SalI SanDI SapI SbfI SfcI SmaI SmlI
SphI TaqII TatI Tth111I XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AloI ApaI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BciVI
BclI BglII BlpI BmtI BpuEI BsaBI BsiWI BsmBI
BspHI BsrBI BstBI BstEII BstXI BstZ17I ClaI CspCI
DraI EciI EcoNI EcoRI EcoRV FalI FseI HpaI
KasI KpnI MfeI NarI NcoI NdeI NheI NotI
NruI PacI PasI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI StyI
SwaI TstI XbaI XcmI XmnI ZraI