(Linear) (Six-base) MAPSORT of: KIBB8327.seq Check: 2090 from: 1 to: 762 KIBB8327.orf With 182 enzymes: SgfI * March 3, 2008 11:05 .. AhdI GACnn_n'nnGTC Cuts at: 0 129 762 Size: 129 633 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 524 762 Size: 524 238 ApaI G_GGCC'C Cuts at: 0 324 762 Size: 324 438 AvaI C'yCGr_G Cuts at: 0 134 176 261 762 Size: 134 42 85 501 Fragments arranged by size: 501 134 85 42 BanII G_rGCy'C Cuts at: 0 63 324 762 Size: 63 261 438 Fragments arranged by size: 438 261 63 BbvCI CC'TCA_GC Cuts at: 0 649 701 762 Size: 649 52 61 Fragments arranged by size: 649 61 52 BclI T'GATC_A Cuts at: 0 191 762 Size: 191 571 BglI GCCn_nnn'nGGC Cuts at: 0 47 762 Size: 47 715 BglII A'GATC_T Cuts at: 0 542 762 Size: 542 220 BlpI GC'TnA_GC Cuts at: 0 37 762 Size: 37 725 Bme1580I G_kGCm'C Cuts at: 0 324 762 Size: 324 438 BmrI ACTGGGnnnn_n' Cuts at: 0 151 762 Size: 151 611 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 77 476 762 Size: 77 399 286 Fragments arranged by size: 399 286 77 Bpu10I CC'TnA_GC Cuts at: 0 649 701 762 Size: 649 52 61 Fragments arranged by size: 649 61 52 BsaI GGTCTCn'nnnn_ Cuts at: 0 376 762 Size: 376 386 BsaAI yAC'GTr Cuts at: 0 110 762 Size: 110 652 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 271 631 634 673 762 Size: 271 360 3 39 89 Fragments arranged by size: 360 271 89 39 3 BseYI C'CCAG_C Cuts at: 0 117 185 201 762 Size: 117 68 16 561 Fragments arranged by size: 561 117 68 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 84 762 Size: 84 678 Bsp1286I G_dGCh'C Cuts at: 0 63 324 762 Size: 63 261 438 Fragments arranged by size: 438 261 63 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 122 762 Size: 122 640 BstYI r'GATC_y Cuts at: 0 542 762 Size: 542 220 BtgI C'CryG_G Cuts at: 0 474 591 611 762 Size: 474 117 20 151 Fragments arranged by size: 474 151 117 20 BtsI GCAGTG_nn' Cuts at: 0 234 755 762 Size: 234 521 7 Fragments arranged by size: 521 234 7 EaeI y'GGCC_r Cuts at: 0 399 762 Size: 399 363 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 501 762 Size: 19 482 261 Fragments arranged by size: 482 261 19 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 77 476 762 Size: 77 399 286 Fragments arranged by size: 399 286 77 EcoO109I rG'GnC_Cy Cuts at: 0 320 762 Size: 320 442 HaeII r_GCGC'y Cuts at: 0 175 372 762 Size: 175 197 390 Fragments arranged by size: 390 197 175 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 50 82 115 147 337 341 369 373 Size: 50 32 33 32 190 4 28 4 Cuts at: 373 762 Size: 389 Fragments arranged by size: 389 190 50 33 32 32 28 4 4 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 359 762 Size: 359 403 MscI TGG'CCA Cuts at: 0 401 762 Size: 401 361 MspA1I CmG'CkG Cuts at: 0 36 189 653 762 Size: 36 153 464 109 Fragments arranged by size: 464 153 109 36 NcoI C'CATG_G Cuts at: 0 591 762 Size: 591 171 NspI r_CATG'y Cuts at: 0 735 762 Size: 735 27 PflMI CCAn_nnn'nTGG Cuts at: 0 444 762 Size: 444 318 PfoI T'CCnGG_A Cuts at: 0 161 555 762 Size: 161 394 207 Fragments arranged by size: 394 207 161 PspOMI G'GGCC_C Cuts at: 0 320 762 Size: 320 442 PvuII CAG'CTG Cuts at: 0 36 189 653 762 Size: 36 153 464 109 Fragments arranged by size: 464 153 109 36 SmaI CCC'GGG Cuts at: 0 136 762 Size: 136 626 StyI C'CwwG_G Cuts at: 0 591 762 Size: 591 171 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 109 467 519 762 Size: 109 358 52 243 Fragments arranged by size: 358 243 109 52 TatI w'GTAC_w Cuts at: 0 12 762 Size: 12 750 Tth111I GACn'n_nGTC Cuts at: 0 157 762 Size: 157 605 XmaI C'CCGG_G Cuts at: 0 134 762 Size: 134 628 Enzymes that do cut: AhdI AlfI ApaI AvaI BanII BbvCI BclI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BseRI BseYI BsgI Bsp1286I BspMI BstYI BtgI BtsI EaeI EciI Eco57MI EcoO109I HaeII Hin4I MmeI MscI MspA1I NcoI NspI PflMI PfoI PspOMI PvuII SmaI StyI TaqII TatI Tth111I XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AleI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BcgI BciVI BfrBI BmgBI BmtI BplI BpuEI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI XbaI XcmI XhoI XmnI ZraI