Restriction map for
pF1KB8328
(Linear) (Six-base) MAPSORT of: KIBB8328.seq Check: 6356 from: 1 to: 519
KIBB8328.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AatII G_ACGT'C
Cuts at: 0 209 519
Size: 209 310
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 477 519
Size: 477 42
AhdI GACnn_n'nnGTC
Cuts at: 0 327 519
Size: 327 192
AleI CACnn'nnGTG
Cuts at: 0 448 519
Size: 448 71
ApaI G_GGCC'C
Cuts at: 0 434 519
Size: 434 85
AvaI C'yCGr_G
Cuts at: 0 233 519
Size: 233 286
BanI G'GyrC_C
Cuts at: 0 22 298 519
Size: 22 276 221
Fragments arranged by size:
276 221 22
BanII G_rGCy'C
Cuts at: 0 86 434 519
Size: 86 348 85
Fragments arranged by size:
348 86 85
BbeI G_GCGC'C
Cuts at: 0 26 519
Size: 26 493
BbsI GAAGACnn'nnnn_
Cuts at: 0 135 519
Size: 135 384
BglII A'GATC_T
Cuts at: 0 155 519
Size: 155 364
BlpI GC'TnA_GC
Cuts at: 0 63 519
Size: 63 456
Bme1580I G_kGCm'C
Cuts at: 0 421 434 519
Size: 421 13 85
Fragments arranged by size:
421 85 13
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 288 519
Size: 100 188 231
Fragments arranged by size:
231 188 100
BsaI GGTCTCn'nnnn_
Cuts at: 0 130 519
Size: 130 389
BsaHI Gr'CG_yC
Cuts at: 0 23 206 519
Size: 23 183 313
Fragments arranged by size:
313 183 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 331 340 519
Size: 331 9 179
Fragments arranged by size:
331 179 9
BsiEI CG_ry'CG
Cuts at: 0 467 519
Size: 467 52
Bsp1286I G_dGCh'C
Cuts at: 0 86 421 434 519
Size: 86 335 13 85
Fragments arranged by size:
335 86 85 13
BstYI r'GATC_y
Cuts at: 0 155 519
Size: 155 364
Bsu36I CC'TnA_GG
Cuts at: 0 343 519
Size: 343 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 22 519
Size: 22 497
EarI CTCTTCn'nnn_
Cuts at: 0 496 519
Size: 496 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 288 477 519
Size: 100 188 189 42
Fragments arranged by size:
189 188 100 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 346 519
Size: 346 173
EcoO109I rG'GnC_Cy
Cuts at: 0 431 519
Size: 431 88
HaeII r_GCGC'y
Cuts at: 0 26 34 290 519
Size: 26 8 256 229
Fragments arranged by size:
256 229 26 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 32 313 345 519
Size: 32 281 32 174
Fragments arranged by size:
281 174 32 32
KasI G'GCGC_C
Cuts at: 0 22 519
Size: 22 497
MslI CAynn'nnrTG
Cuts at: 0 448 519
Size: 448 71
MspA1I CmG'CkG
Cuts at: 0 59 519
Size: 59 460
NarI GG'CG_CC
Cuts at: 0 23 519
Size: 23 496
NspI r_CATG'y
Cuts at: 0 170 519
Size: 170 349
PasI CC'CwG_GG
Cuts at: 0 434 519
Size: 434 85
PflMI CCAn_nnn'nTGG
Cuts at: 0 189 246 519
Size: 189 57 273
Fragments arranged by size:
273 189 57
PfoI T'CCnGG_A
Cuts at: 0 265 519
Size: 265 254
PspOMI G'GGCC_C
Cuts at: 0 430 519
Size: 430 89
PspXI vC'TCGA_Gb
Cuts at: 0 233 519
Size: 233 286
PvuI CG_AT'CG
Cuts at: 0 467 519
Size: 467 52
SapI GCTCTTCn'nnn_
Cuts at: 0 496 519
Size: 496 23
SfcI C'TryA_G
Cuts at: 0 333 519
Size: 333 186
SfoI GGC'GCC
Cuts at: 0 24 519
Size: 24 495
SmlI C'TyrA_G
Cuts at: 0 233 519
Size: 233 286
StuI AGG'CCT
Cuts at: 0 73 519
Size: 73 446
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 426 519
Size: 426 93
Tth111I GACn'n_nGTC
Cuts at: 0 121 519
Size: 121 398
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 450 519
Size: 450 69
XhoI C'TCGA_G
Cuts at: 0 233 519
Size: 233 286
ZraI GAC'GTC
Cuts at: 0 207 519
Size: 207 312
Enzymes that do cut:
AatII AcuI AhdI AleI ApaI AvaI BanI BanII
BbeI BbsI BglII BlpI Bme1580I BpmI BsaI BsaHI
BseRI BsiEI Bsp1286I BstYI Bsu36I BtgZI EarI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI MslI MspA1I NarI
NspI PasI PflMI PfoI PspOMI PspXI PvuI SapI
SfcI SfoI SmlI StuI TaqII Tth111I XcmI XhoI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI
BsaBI BsaWI BsaXI BseYI BsgI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PstI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StyI SwaI TatI TstI
XbaI XmaI XmnI