(Linear) (Six-base) MAPSORT of: KIBB8331.seq Check: 5791 from: 1 to: 630 KIBB8331.orf With 182 enzymes: SgfI * July 2, 2008 13:23 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 388 630 Size: 388 242 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 215 630 Size: 215 415 AvaI C'yCGr_G Cuts at: 0 134 159 329 562 630 Size: 134 25 170 233 68 Fragments arranged by size: 233 170 134 68 25 BamHI G'GATC_C Cuts at: 0 320 466 630 Size: 320 146 164 Fragments arranged by size: 320 164 146 BanI G'GyrC_C Cuts at: 0 112 175 199 224 630 Size: 112 63 24 25 406 Fragments arranged by size: 406 112 63 25 24 BanII G_rGCy'C Cuts at: 0 573 630 Size: 573 57 BbeI G_GCGC'C Cuts at: 0 179 630 Size: 179 451 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 140 174 576 610 630 Size: 140 34 402 34 20 Fragments arranged by size: 402 140 34 34 20 BglI GCCn_nnn'nGGC Cuts at: 0 56 83 630 Size: 56 27 547 Fragments arranged by size: 547 56 27 BglII A'GATC_T Cuts at: 0 498 630 Size: 498 132 Bme1580I G_kGCm'C Cuts at: 0 22 229 630 Size: 22 207 401 Fragments arranged by size: 401 207 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 283 630 Size: 283 347 BsaI GGTCTCn'nnnn_ Cuts at: 0 528 630 Size: 528 102 BsaHI Gr'CG_yC Cuts at: 0 176 630 Size: 176 454 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 185 630 Size: 185 445 BseYI C'CCAG_C Cuts at: 0 433 462 630 Size: 433 29 168 Fragments arranged by size: 433 168 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 606 630 Size: 606 24 BsmI GAATG_Cn' Cuts at: 0 65 630 Size: 65 565 Bsp1286I G_dGCh'C Cuts at: 0 22 229 573 630 Size: 22 207 344 57 Fragments arranged by size: 344 207 57 22 BsrBI CCG'CTC Cuts at: 0 345 630 Size: 345 285 BsrDI GCAATG_nn' Cuts at: 0 93 630 Size: 93 537 BstXI CCAn_nnnn'nTGG Cuts at: 0 580 630 Size: 580 50 BstYI r'GATC_y Cuts at: 0 320 466 498 630 Size: 320 146 32 132 Fragments arranged by size: 320 146 132 32 BtgI C'CryG_G Cuts at: 0 116 181 296 630 Size: 116 65 115 334 Fragments arranged by size: 334 116 115 65 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 53 630 Size: 53 577 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 453 459 630 Size: 453 6 171 Fragments arranged by size: 453 171 6 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 388 630 Size: 388 242 EcoO109I rG'GnC_Cy Cuts at: 0 58 130 155 630 Size: 58 72 25 475 Fragments arranged by size: 475 72 58 25 FspI TGC'GCA Cuts at: 0 212 630 Size: 212 418 HaeII r_GCGC'y Cuts at: 0 179 434 630 Size: 179 255 196 Fragments arranged by size: 255 196 179 KasI G'GCGC_C Cuts at: 0 175 630 Size: 175 455 MslI CAynn'nnrTG Cuts at: 0 197 277 630 Size: 197 80 353 Fragments arranged by size: 353 197 80 MspA1I CmG'CkG Cuts at: 0 183 630 Size: 183 447 NarI GG'CG_CC Cuts at: 0 176 630 Size: 176 454 NcoI C'CATG_G Cuts at: 0 296 630 Size: 296 334 PasI CC'CwG_GG Cuts at: 0 79 573 630 Size: 79 494 57 Fragments arranged by size: 494 79 57 PpuMI rG'GwC_Cy Cuts at: 0 130 630 Size: 130 500 SacII CC_GC'GG Cuts at: 0 184 630 Size: 184 446 SexAI A'CCwGG_T Cuts at: 0 537 630 Size: 537 93 SfiI GGCCn_nnn'nGGCC Cuts at: 0 56 630 Size: 56 574 SfoI GGC'GCC Cuts at: 0 177 630 Size: 177 453 SmaI CCC'GGG Cuts at: 0 136 331 630 Size: 136 195 299 Fragments arranged by size: 299 195 136 SmlI C'TyrA_G Cuts at: 0 262 630 Size: 262 368 StuI AGG'CCT Cuts at: 0 523 630 Size: 523 107 StyI C'CwwG_G Cuts at: 0 296 630 Size: 296 334 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 525 572 630 Size: 525 47 58 Fragments arranged by size: 525 58 47 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 208 240 630 Size: 208 32 390 Fragments arranged by size: 390 208 32 XmaI C'CCGG_G Cuts at: 0 134 329 630 Size: 134 195 301 Fragments arranged by size: 301 195 134 XmnI GAAnn'nnTTC Cuts at: 0 373 630 Size: 373 257 Enzymes that do cut: AcuI AlfI AvaI BamHI BanI BanII BbeI BcgI BglI BglII Bme1580I BpuEI BsaI BsaHI BseRI BseYI BsgI BsmI Bsp1286I BsrBI BsrDI BstXI BstYI BtgI BtgZI EciI Eco57MI EcoO109I FspI HaeII KasI MslI MspA1I NarI NcoI PasI PpuMI SacII SexAI SfiI SfoI SmaI SmlI StuI StyI TaqII TstI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI BbvCI BciVI BclI BfrBI BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I BsaAI BsaBI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XcmI XhoI ZraI