(Linear) (Six-base) MAPSORT of: KIBB8334.seq  Check: 3448  from: 1  to: 564



KIBB8334.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AvaI C'yCGr_G

Cuts at:      0     423     564 
   Size:        423     141
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     236     564 
   Size:        236     328
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       1      35      64      98     315     349     564 
   Size:          1      34      29      34     217      34     215

  Fragments arranged by size: 

                 217     215      34      34      34      29       1
 
BglII A'GATC_T

Cuts at:      0     460     564 
   Size:        460     104
 
Bme1580I G_kGCm'C

Cuts at:      0     421     564 
   Size:        421     143
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     564 
   Size:        295     269
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      21     564 
   Size:         21     543
 
BsaHI Gr'CG_yC

Cuts at:      0      14     564 
   Size:         14     550
 
BsiHKAI G_wGCw'C

Cuts at:      0      45     564 
   Size:         45     519
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0       2     564 
   Size:          2     562
 
Bsp1286I G_dGCh'C

Cuts at:      0      45     421     564 
   Size:         45     376     143

  Fragments arranged by size: 

                 376     143      45
 
BssSI C'ACGA_G

Cuts at:      0     454     564 
   Size:        454     110
 
BstYI r'GATC_y

Cuts at:      0      59     460     564 
   Size:         59     401     104

  Fragments arranged by size: 

                 401     104      59
 
Bsu36I CC'TnA_GG

Cuts at:      0     548     564 
   Size:        548      16
 
BtgI C'CryG_G

Cuts at:      0      32     267     564 
   Size:         32     235     297

  Fragments arranged by size: 

                 297     235      32
 
EarI CTCTTCn'nnn_

Cuts at:      0      98     148     527     564 
   Size:         98      50     379      37

  Fragments arranged by size: 

                 379      98      50      37
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     564 
   Size:        295     269
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      21     564 
   Size:         21     543
 
MspA1I CmG'CkG

Cuts at:      0     519     564 
   Size:        519      45
 
NspI r_CATG'y

Cuts at:      0     487     564 
   Size:        487      77
 
PvuII CAG'CTG

Cuts at:      0     519     564 
   Size:        519      45
 
SmaI CCC'GGG

Cuts at:      0     425     564 
   Size:        425     139
 
StuI AGG'CCT

Cuts at:      0      70     564 
   Size:         70     494
 
XmaI C'CCGG_G

Cuts at:      0     423     564 
   Size:        423     141
 
XmnI GAAnn'nnTTC

Cuts at:      0     136     363     564 
   Size:        136     227     201

  Fragments arranged by size: 

                 227     201     136
 

 Enzymes that do cut:

     AvaI     BbsI     BcgI    BglII Bme1580I     BpmI     BsaI    BsaHI
  BsiHKAI    BsmBI Bsp1286I    BssSI    BstYI   Bsu36I     BtgI     EarI
  Eco57MI    EcoNI   MspA1I     NspI    PvuII     SmaI     StuI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     ZraI