(Linear) (Six-base) MAPSORT of: KIBB8335.seq Check: 3729 from: 1 to: 1320 KIBB8335.orf With 182 enzymes: SgfI * July 2, 2008 13:23 .. AccI GT'mk_AC Cuts at: 0 413 1320 Size: 413 907 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 56 574 1320 Size: 56 518 746 Fragments arranged by size: 746 518 56 AfeI AGC'GCT Cuts at: 0 1210 1320 Size: 1210 110 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 520 1070 1320 Size: 520 550 250 Fragments arranged by size: 550 520 250 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 343 375 1320 Size: 343 32 945 Fragments arranged by size: 945 343 32 AlwNI CAG_nnn'CTG Cuts at: 0 180 513 1101 1320 Size: 180 333 588 219 Fragments arranged by size: 588 333 219 180 ApaLI G'TGCA_C Cuts at: 0 319 1320 Size: 319 1001 ApoI r'AATT_y Cuts at: 0 538 1320 Size: 538 782 AvaI C'yCGr_G Cuts at: 0 25 1320 Size: 25 1295 BamHI G'GATC_C Cuts at: 0 484 1320 Size: 484 836 BanII G_rGCy'C Cuts at: 0 85 170 1320 Size: 85 85 1150 Fragments arranged by size: 1150 85 85 BbsI GAAGACnn'nnnn_ Cuts at: 0 398 1320 Size: 398 922 BlpI GC'TnA_GC Cuts at: 0 628 763 811 1320 Size: 628 135 48 509 Fragments arranged by size: 628 509 135 48 Bme1580I G_kGCm'C Cuts at: 0 323 1320 Size: 323 997 BmrI ACTGGGnnnn_n' Cuts at: 0 1275 1320 Size: 1275 45 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 99 1320 Size: 99 1221 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 136 688 702 1042 1054 1148 1320 Size: 136 552 14 340 12 94 172 Fragments arranged by size: 552 340 172 136 94 14 12 Bpu10I CC'TnA_GC Cuts at: 0 824 1320 Size: 824 496 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 636 853 1320 Size: 636 217 467 Fragments arranged by size: 636 467 217 BsaI GGTCTCn'nnnn_ Cuts at: 0 1123 1320 Size: 1123 197 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 316 343 346 373 954 984 1320 Size: 316 27 3 27 581 30 336 Fragments arranged by size: 581 336 316 30 27 27 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 232 1320 Size: 232 1088 BseYI C'CCAG_C Cuts at: 0 965 1320 Size: 965 355 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1214 1320 Size: 1214 106 BsiHKAI G_wGCw'C Cuts at: 0 323 1320 Size: 323 997 Bsp1286I G_dGCh'C Cuts at: 0 85 170 323 1320 Size: 85 85 153 997 Fragments arranged by size: 997 153 85 85 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 650 812 1109 1320 Size: 650 162 297 211 Fragments arranged by size: 650 297 211 162 BsrBI CCG'CTC Cuts at: 0 1017 1170 1320 Size: 1017 153 150 Fragments arranged by size: 1017 153 150 BsrFI r'CCGG_y Cuts at: 0 686 1320 Size: 686 634 BsrGI T'GTAC_A Cuts at: 0 797 1320 Size: 797 523 BssHII G'CGCG_C Cuts at: 0 829 1320 Size: 829 491 BssSI C'ACGA_G Cuts at: 0 325 1320 Size: 325 995 BstAPI GCAn_nnn'nTGC Cuts at: 0 513 1101 1250 1320 Size: 513 588 149 70 Fragments arranged by size: 588 513 149 70 BstEII G'GTnAC_C Cuts at: 0 186 1320 Size: 186 1134 BstXI CCAn_nnnn'nTGG Cuts at: 0 312 1010 1320 Size: 312 698 310 Fragments arranged by size: 698 312 310 BstYI r'GATC_y Cuts at: 0 484 998 1320 Size: 484 514 322 Fragments arranged by size: 514 484 322 Bsu36I CC'TnA_GG Cuts at: 0 473 818 1320 Size: 473 345 502 Fragments arranged by size: 502 473 345 BtgI C'CryG_G Cuts at: 0 192 1320 Size: 192 1128 BtsI GCAGTG_nn' Cuts at: 0 236 895 1163 1197 1320 Size: 236 659 268 34 123 Fragments arranged by size: 659 268 236 123 34 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 194 229 1320 Size: 194 35 1091 Fragments arranged by size: 1091 194 35 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 56 136 574 688 702 1042 1054 1148 Size: 56 80 438 114 14 340 12 94 Cuts at: 1148 1320 Size: 172 Fragments arranged by size: 438 340 172 114 94 80 56 14 12 EcoO109I rG'GnC_Cy Cuts at: 0 201 558 981 1098 1320 Size: 201 357 423 117 222 Fragments arranged by size: 423 357 222 201 117 FspI TGC'GCA Cuts at: 0 58 1320 Size: 58 1262 HaeII r_GCGC'y Cuts at: 0 1212 1320 Size: 1212 108 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 586 618 851 883 1320 Size: 586 32 233 32 437 Fragments arranged by size: 586 437 233 32 32 HindIII A'AGCT_T Cuts at: 0 871 1320 Size: 871 449 MfeI C'AATT_G Cuts at: 0 1052 1320 Size: 1052 268 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 752 971 1320 Size: 752 219 349 Fragments arranged by size: 752 349 219 MslI CAynn'nnrTG Cuts at: 0 310 316 1320 Size: 310 6 1004 Fragments arranged by size: 1004 310 6 MspA1I CmG'CkG Cuts at: 0 102 909 1178 1320 Size: 102 807 269 142 Fragments arranged by size: 807 269 142 102 NaeI GCC'GGC Cuts at: 0 688 1320 Size: 688 632 NcoI C'CATG_G Cuts at: 0 192 1320 Size: 192 1128 NgoMIV G'CCGG_C Cuts at: 0 686 1320 Size: 686 634 NspI r_CATG'y Cuts at: 0 132 1078 1320 Size: 132 946 242 Fragments arranged by size: 946 242 132 PasI CC'CwG_GG Cuts at: 0 1302 1320 Size: 1302 18 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 343 375 1320 Size: 343 32 945 Fragments arranged by size: 945 343 32 PpuMI rG'GwC_Cy Cuts at: 0 201 981 1098 1320 Size: 201 780 117 222 Fragments arranged by size: 780 222 201 117 PshAI GACnn'nnGTC Cuts at: 0 1288 1320 Size: 1288 32 PstI C_TGCA'G Cuts at: 0 271 518 710 1112 1192 1246 1320 Size: 271 247 192 402 80 54 74 Fragments arranged by size: 402 271 247 192 80 74 54 PvuII CAG'CTG Cuts at: 0 909 1320 Size: 909 411 ScaI AGT'ACT Cuts at: 0 569 1320 Size: 569 751 SexAI A'CCwGG_T Cuts at: 0 983 1320 Size: 983 337 SfcI C'TryA_G Cuts at: 0 267 514 706 1108 1134 1188 1242 1320 Size: 267 247 192 402 26 54 54 78 Fragments arranged by size: 402 267 247 192 78 54 54 26 SmaI CCC'GGG Cuts at: 0 27 1320 Size: 27 1293 SmlI C'TyrA_G Cuts at: 0 615 868 1320 Size: 615 253 452 Fragments arranged by size: 615 452 253 StuI AGG'CCT Cuts at: 0 331 1320 Size: 331 989 StyI C'CwwG_G Cuts at: 0 192 854 1320 Size: 192 662 466 Fragments arranged by size: 662 466 192 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 205 1320 Size: 205 1115 TatI w'GTAC_w Cuts at: 0 567 797 1320 Size: 567 230 523 Fragments arranged by size: 567 523 230 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 316 348 1320 Size: 316 32 972 Fragments arranged by size: 972 316 32 Tth111I GACn'n_nGTC Cuts at: 0 397 1320 Size: 397 923 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 312 457 1320 Size: 312 145 863 Fragments arranged by size: 863 312 145 XmaI C'CCGG_G Cuts at: 0 25 1320 Size: 25 1295 XmnI GAAnn'nnTTC Cuts at: 0 253 298 1320 Size: 253 45 1022 Fragments arranged by size: 1022 253 45 Enzymes that do cut: AccI AcuI AfeI AlfI AloI AlwNI ApaLI ApoI AvaI BamHI BanII BbsI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI Eco57MI EcoO109I FspI HaeII Hin4I HindIII MfeI MmeI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PpiI PpuMI PshAI PstI PvuII ScaI SexAI SfcI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AflIII AgeI AhdI AleI ApaI AscI AseI AsiSI AvrII BaeI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BmgBI BmtI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BstBI BstZ17I BtgZI ClaI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HincII HpaI KasI KpnI MluI MscI NarI NdeI NheI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI ZraI