Restriction map for
pF1KB8336
(Linear) (Six-base) MAPSORT of: KIBB8336.seq Check: 2378 from: 1 to: 1473
KIBB8336.orf
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 713 897 981 1065 1149 1233 1401
Size: 605 108 184 84 84 84 84 168
Cuts at: 1401 1473
Size: 72
Fragments arranged by size:
605 184 168 108 84 84 84 84
72
AflIII A'CryG_T
Cuts at: 0 1148 1232 1473
Size: 1148 84 241
Fragments arranged by size:
1148 241 84
AlwNI CAG_nnn'CTG
Cuts at: 0 231 264 291 390 1256 1403 1473
Size: 231 33 27 99 866 147 70
Fragments arranged by size:
866 231 147 99 70 33 27
ApaLI G'TGCA_C
Cuts at: 0 1117 1473
Size: 1117 356
ApoI r'AATT_y
Cuts at: 0 739 1473
Size: 739 734
AvaI C'yCGr_G
Cuts at: 0 238 675 847 1473
Size: 238 437 172 626
Fragments arranged by size:
626 437 238 172
BamHI G'GATC_C
Cuts at: 0 408 1367 1451 1473
Size: 408 959 84 22
Fragments arranged by size:
959 408 84 22
BanI G'GyrC_C
Cuts at: 0 670 1473
Size: 670 803
BanII G_rGCy'C
Cuts at: 0 28 333 425 1345 1473
Size: 28 305 92 920 128
Fragments arranged by size:
920 305 128 92 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 740 868 883 1473
Size: 740 128 15 590
Fragments arranged by size:
740 590 128 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 200 234 912 946 1473
Size: 200 34 678 34 527
Fragments arranged by size:
678 527 200 34 34
BglII A'GATC_T
Cuts at: 0 575 1473
Size: 575 898
BlpI GC'TnA_GC
Cuts at: 0 1260 1428 1473
Size: 1260 168 45
Fragments arranged by size:
1260 168 45
Bme1580I G_kGCm'C
Cuts at: 0 1121 1473
Size: 1121 352
BmrI ACTGGGnnnn_n'
Cuts at: 0 22 1473
Size: 22 1451
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 264 564 1473
Size: 264 300 909
Fragments arranged by size:
909 300 264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 301 1061 1313 1473
Size: 286 15 760 252 160
Fragments arranged by size:
760 286 252 160 15
Bpu10I CC'TnA_GC
Cuts at: 0 22 1473
Size: 22 1451
BsaI GGTCTCn'nnnn_
Cuts at: 0 478 1473
Size: 478 995
BsaBI GATnn'nnATC
Cuts at: 0 1456 1473
Size: 1456 17
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 694 724 756 786 1473
Size: 694 30 32 30 687
Fragments arranged by size:
694 687 32 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 187 1335 1473
Size: 85 102 1148 138
Fragments arranged by size:
1148 138 102 85
BseYI C'CCAG_C
Cuts at: 0 935 1103 1473
Size: 935 168 370
Fragments arranged by size:
935 370 168
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 1416 1473
Size: 125 1291 57
Fragments arranged by size:
1291 125 57
BsiHKAI G_wGCw'C
Cuts at: 0 925 1121 1345 1361 1473
Size: 925 196 224 16 112
Fragments arranged by size:
925 224 196 112 16
Bsp1286I G_dGCh'C
Cuts at: 0 28 333 425 925 1121 1345 1361 1473
Size: 28 305 92 500 196 224 16 112
Fragments arranged by size:
500 305 224 196 112 92 28 16
BsrBI CCG'CTC
Cuts at: 0 347 1131 1473
Size: 347 784 342
Fragments arranged by size:
784 347 342
BsrDI GCAATG_nn'
Cuts at: 0 122 1473
Size: 122 1351
BssSI C'ACGA_G
Cuts at: 0 190 1473
Size: 190 1283
BstXI CCAn_nnnn'nTGG
Cuts at: 0 35 1473
Size: 35 1438
BstYI r'GATC_y
Cuts at: 0 311 375 408 575 602 1367 1451 1473
Size: 311 64 33 167 27 765 84 22
Fragments arranged by size:
765 311 167 84 64 33 27 22
Bsu36I CC'TnA_GG
Cuts at: 0 539 1473
Size: 539 934
BtgI C'CryG_G
Cuts at: 0 34 1473
Size: 34 1439
EaeI y'GGCC_r
Cuts at: 0 2 657 697 1239 1473
Size: 2 655 40 542 234
Fragments arranged by size:
655 542 234 40 2
EarI CTCTTCn'nnn_
Cuts at: 0 35 71 787 1473
Size: 35 36 716 686
Fragments arranged by size:
716 686 36 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 116 1473
Size: 116 1357
EcoICRI GAG'CTC
Cuts at: 0 1343 1473
Size: 1343 130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 301 605 713 897 981 1061 1065
Size: 286 15 304 108 184 84 80 4
Cuts at: 1065 1149 1233 1313 1401 1473
Size: 84 84 80 88 72
Fragments arranged by size:
304 286 184 108 88 84 84 84
80 80 72 15 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 94 1473
Size: 94 1379
EcoO109I rG'GnC_Cy
Cuts at: 0 195 625 797 1473
Size: 195 430 172 676
Fragments arranged by size:
676 430 195 172
HaeII r_GCGC'y
Cuts at: 0 847 1473
Size: 847 626
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 163 195 756 788 855 887 1260 1292
Size: 163 32 561 32 67 32 373 32
Cuts at: 1292 1473
Size: 181
Fragments arranged by size:
561 373 181 163 67 32 32 32
32
MluI A'CGCG_T
Cuts at: 0 1148 1232 1473
Size: 1148 84 241
Fragments arranged by size:
1148 241 84
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1296 1473
Size: 1296 177
MscI TGG'CCA
Cuts at: 0 4 699 1473
Size: 4 695 774
Fragments arranged by size:
774 695 4
MslI CAynn'nnrTG
Cuts at: 0 1308 1462 1473
Size: 1308 154 11
Fragments arranged by size:
1308 154 11
MspA1I CmG'CkG
Cuts at: 0 361 1113 1473
Size: 361 752 360
Fragments arranged by size:
752 361 360
NdeI CA'TA_TG
Cuts at: 0 885 1473
Size: 885 588
NruI TCG'CGA
Cuts at: 0 812 1473
Size: 812 661
NspI r_CATG'y
Cuts at: 0 533 1473
Size: 533 940
PasI CC'CwG_GG
Cuts at: 0 96 1473
Size: 96 1377
PfoI T'CCnGG_A
Cuts at: 0 411 1473
Size: 411 1062
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 669 701 1473
Size: 669 32 772
Fragments arranged by size:
772 669 32
PpuMI rG'GwC_Cy
Cuts at: 0 797 1473
Size: 797 676
PsiI TTA'TAA
Cuts at: 0 1391 1473
Size: 1391 82
PspXI vC'TCGA_Gb
Cuts at: 0 675 847 1473
Size: 675 172 626
Fragments arranged by size:
675 626 172
PstI C_TGCA'G
Cuts at: 0 236 895 1473
Size: 236 659 578
Fragments arranged by size:
659 578 236
SacI G_AGCT'C
Cuts at: 0 1345 1473
Size: 1345 128
SexAI A'CCwGG_T
Cuts at: 0 941 1473
Size: 941 532
SfcI C'TryA_G
Cuts at: 0 232 891 1473
Size: 232 659 582
Fragments arranged by size:
659 582 232
SmlI C'TyrA_G
Cuts at: 0 675 847 1473
Size: 675 172 626
Fragments arranged by size:
675 626 172
StuI AGG'CCT
Cuts at: 0 994 1330 1414 1473
Size: 994 336 84 59
Fragments arranged by size:
994 336 84 59
StyI C'CwwG_G
Cuts at: 0 251 507 1473
Size: 251 256 966
Fragments arranged by size:
966 256 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 283 312 610 1024 1117 1473
Size: 283 29 298 414 93 356
Fragments arranged by size:
414 356 298 283 93 29
Tth111I GACn'n_nGTC
Cuts at: 0 585 1473
Size: 585 888
XhoI C'TCGA_G
Cuts at: 0 675 847 1473
Size: 675 172 626
Fragments arranged by size:
675 626 172
XmnI GAAnn'nnTTC
Cuts at: 0 826 910 994 1162 1414 1473
Size: 826 84 84 168 252 59
Fragments arranged by size:
826 252 168 84 84 59
Enzymes that do cut:
AcuI AflIII AlwNI ApaLI ApoI AvaI BamHI BanI
BanII BbsI BcgI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BsaI BsaBI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BsrBI BsrDI BssSI BstXI BstYI Bsu36I
BtgI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I MluI MmeI MscI MslI MspA1I NdeI
NruI NspI PasI PfoI PpiI PpuMI PsiI PspXI
PstI SacI SexAI SfcI SmlI StuI StyI TaqII
Tth111I XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BciVI BclI BfrBI BglI
BmgBI BmtI BpuEI BsaAI BsaHI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
NaeI NarI NcoI NgoMIV NheI NotI NsiI PacI
PciI PflMI PmeI PmlI PshAI PspOMI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TatI TstI XbaI XcmI XmaI
ZraI