(Linear) (Six-base) MAPSORT of: KIBB8341.seq  Check: 8928  from: 1  to: 1827



KIBB8341.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     521    1827 
   Size:        521    1306
 
AflIII A'CryG_T

Cuts at:      0     320    1759    1827 
   Size:        320    1439      68

  Fragments arranged by size: 

                1439     320      68
 
AleI CACnn'nnGTG

Cuts at:      0     204     569    1574    1827 
   Size:        204     365    1005     253

  Fragments arranged by size: 

                1005     365     253     204
 
ApoI r'AATT_y

Cuts at:      0     560     644     812     896    1064    1232    1316    1400 
   Size:        560      84     168      84     168     168      84      84

Cuts at:   1400    1481    1565    1827 
   Size:         81      84     262

  Fragments arranged by size: 

                 560     262     168     168     168      84      84      84
                  84      84      81
 
BamHI G'GATC_C

Cuts at:      0    1733    1827 
   Size:       1733      94
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1114    1447    1827 
   Size:       1114     333     380

  Fragments arranged by size: 

                1114     380     333
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     155     187    1679    1827 
   Size:        155      32    1492     148

  Fragments arranged by size: 

                1492     155     148      32
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1158    1827 
   Size:       1158     669
 
BsaAI yAC'GTr

Cuts at:      0    1453    1827 
   Size:       1453     374
 
BsaWI w'CCGG_w

Cuts at:      0    1240    1827 
   Size:       1240     587
 
BsmI GAATG_Cn'

Cuts at:      0      67     433    1105    1827 
   Size:         67     366     672     722

  Fragments arranged by size: 

                 722     672     366      67
 
BsrGI T'GTAC_A

Cuts at:      0      89     413    1827 
   Size:         89     324    1414

  Fragments arranged by size: 

                1414     324      89
 
BstYI r'GATC_y

Cuts at:      0    1733    1827 
   Size:       1733      94
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1167    1202    1827 
   Size:       1167      35     625

  Fragments arranged by size: 

                1167     625      35
 
EaeI y'GGCC_r

Cuts at:      0      32    1827 
   Size:         32    1795
 
EarI CTCTTCn'nnn_

Cuts at:      0     378     806    1827 
   Size:        378     428    1021

  Fragments arranged by size: 

                1021     428     378
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     155     187     521    1679    1827 
   Size:        155      32     334    1158     148

  Fragments arranged by size: 

                1158     334     155     148      32
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1694    1827 
   Size:       1694     133
 
EcoRI G'AATT_C

Cuts at:      0     560     812     896    1064    1232    1316    1481    1565 
   Size:        560     252      84     168     168      84     165      84

Cuts at:   1565    1827 
   Size:        262

  Fragments arranged by size: 

                 560     262     252     168     168     165      84      84
                  84
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     127     159    1827 
   Size:        127      32    1668

  Fragments arranged by size: 

                1668     127      32
 
HindIII A'AGCT_T

Cuts at:      0     689    1827 
   Size:        689    1138
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      86    1827 
   Size:         86    1741
 
MscI TGG'CCA

Cuts at:      0      34    1827 
   Size:         34    1793
 
MslI CAynn'nnrTG

Cuts at:      0     204     569     653     753     821     905     989    1157 
   Size:        204     365      84     100      68      84      84     168

Cuts at:   1157    1325    1490    1574    1742    1827 
   Size:        168     165      84     168      85

  Fragments arranged by size: 

                 365     204     168     168     168     165     100      85
                  84      84      84      84      68
 
NdeI CA'TA_TG

Cuts at:      0     750    1006    1827 
   Size:        750     256     821

  Fragments arranged by size: 

                 821     750     256
 
NspI r_CATG'y

Cuts at:      0     324     683    1763    1827 
   Size:        324     359    1080      64

  Fragments arranged by size: 

                1080     359     324      64
 
PciI A'CATG_T

Cuts at:      0     320    1759    1827 
   Size:        320    1439      68

  Fragments arranged by size: 

                1439     320      68
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      12    1827 
   Size:         12    1815
 
PsiI TTA'TAA

Cuts at:      0    1172    1827 
   Size:       1172     655
 
SfcI C'TryA_G

Cuts at:      0     114    1827 
   Size:        114    1713
 
StuI AGG'CCT

Cuts at:      0     607     859     943    1279    1363    1528    1827 
   Size:        607     252      84     336      84     165     299

  Fragments arranged by size: 

                 607     336     299     252     165      84      84
 
StyI C'CwwG_G

Cuts at:      0     401    1827 
   Size:        401    1426
 
TatI w'GTAC_w

Cuts at:      0      89     413    1827 
   Size:         89     324    1414

  Fragments arranged by size: 

                1414     324      89
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     ApoI    BamHI     BbsI     BpmI     BsaI
    BsaAI    BsaWI     BsmI    BsrGI    BstYI    CspCI     EaeI     EarI
  Eco57MI EcoO109I    EcoRI    Hin4I  HindIII     MmeI     MscI     MslI
     NdeI     NspI     PciI    PflMI     PsiI     SfcI     StuI     StyI
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaBI    BsaHI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MfeI     MluI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI