Restriction map for
pF1KB8345
(Linear) (Six-base) MAPSORT of: KIBB8345.seq Check: 1040 from: 1 to: 1884
KIBB8345.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AclI AA'CG_TT
Cuts at: 0 808 1884
Size: 808 1076
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 945 1365 1445 1808 1884
Size: 945 420 80 363 76
Fragments arranged by size:
945 420 363 80 76
AflII C'TTAA_G
Cuts at: 0 1734 1884
Size: 1734 150
AflIII A'CryG_T
Cuts at: 0 437 1407 1575 1743 1884
Size: 437 970 168 168 141
Fragments arranged by size:
970 437 168 168 141
AlwNI CAG_nnn'CTG
Cuts at: 0 117 1884
Size: 117 1767
ApaLI G'TGCA_C
Cuts at: 0 1249 1884
Size: 1249 635
ApoI r'AATT_y
Cuts at: 0 257 404 1807 1884
Size: 257 147 1403 77
Fragments arranged by size:
1403 257 147 77
BbsI GAAGACnn'nnnn_
Cuts at: 0 444 822 906 990 1074 1129 1158 1433
Size: 444 378 84 84 84 55 29 275
Cuts at: 1433 1623 1884
Size: 190 261
Fragments arranged by size:
444 378 275 261 190 84 84 84
55 29
BbvCI CC'TCA_GC
Cuts at: 0 370 1884
Size: 370 1514
BclI T'GATC_A
Cuts at: 0 351 1853 1884
Size: 351 1502 31
Fragments arranged by size:
1502 351 31
BfrBI ATG'CAT
Cuts at: 0 459 567 1884
Size: 459 108 1317
Fragments arranged by size:
1317 459 108
BglII A'GATC_T
Cuts at: 0 1848 1884
Size: 1848 36
Bme1580I G_kGCm'C
Cuts at: 0 1253 1884
Size: 1253 631
BmrI ACTGGGnnnn_n'
Cuts at: 0 1134 1884
Size: 1134 750
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 385 1884
Size: 385 1499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 121 434 941 1025 1039 1109 1277
Size: 64 57 313 507 84 14 70 168
Cuts at: 1277 1697 1884
Size: 420 187
Fragments arranged by size:
507 420 313 187 168 84 70 64
57 14
Bpu10I CC'TnA_GC
Cuts at: 0 370 1884
Size: 370 1514
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 705 1884
Size: 460 245 1179
Fragments arranged by size:
1179 460 245
BsaI GGTCTCn'nnnn_
Cuts at: 0 97 585 1884
Size: 97 488 1299
Fragments arranged by size:
1299 488 97
BsaAI yAC'GTr
Cuts at: 0 440 1884
Size: 440 1444
BsaWI w'CCGG_w
Cuts at: 0 751 1884
Size: 751 1133
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1210 1240 1884
Size: 1210 30 644
Fragments arranged by size:
1210 644 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 1215 1884
Size: 61 1154 669
Fragments arranged by size:
1154 669 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1654 1836 1884
Size: 1654 182 48
Fragments arranged by size:
1654 182 48
BsiHKAI G_wGCw'C
Cuts at: 0 1253 1884
Size: 1253 631
BsiWI C'GTAC_G
Cuts at: 0 440 1884
Size: 440 1444
BsmI GAATG_Cn'
Cuts at: 0 569 1027 1111 1195 1210 1421 1447 1714
Size: 569 458 84 84 15 211 26 267
Cuts at: 1714 1884
Size: 170
Fragments arranged by size:
569 458 267 211 170 84 84 26
15
Bsp1286I G_dGCh'C
Cuts at: 0 1253 1884
Size: 1253 631
BsrDI GCAATG_nn'
Cuts at: 0 700 1288 1884
Size: 700 588 596
Fragments arranged by size:
700 596 588
BsrGI T'GTAC_A
Cuts at: 0 395 1884
Size: 395 1489
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1199 1884
Size: 1199 685
BstBI TT'CG_AA
Cuts at: 0 813 897 981 1065 1149 1884
Size: 813 84 84 84 84 735
Fragments arranged by size:
813 735 84 84 84 84
BstXI CCAn_nnnn'nTGG
Cuts at: 0 529 1884
Size: 529 1355
BstYI r'GATC_y
Cuts at: 0 1848 1884
Size: 1848 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 445 1884
Size: 445 1439
BtsI GCAGTG_nn'
Cuts at: 0 665 1372 1540 1824 1884
Size: 665 707 168 284 60
Fragments arranged by size:
707 665 284 168 60
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1014 1049 1098 1133 1266 1301 1434 1469
Size: 1014 35 49 35 133 35 133 35
Cuts at: 1469 1518 1553 1884
Size: 49 35 331
Fragments arranged by size:
1014 331 133 133 49 49 35 35
35 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 10 1884
Size: 10 1874
EaeI y'GGCC_r
Cuts at: 0 1639 1884
Size: 1639 245
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 121 434 941 945 1025 1039 1109
Size: 64 57 313 507 4 80 14 70
Cuts at: 1109 1277 1365 1445 1697 1808 1884
Size: 168 88 80 252 111 76
Fragments arranged by size:
507 313 252 168 111 88 80 80
76 70 64 57 14 4
EcoO109I rG'GnC_Cy
Cuts at: 0 78 1884
Size: 78 1806
EcoRI G'AATT_C
Cuts at: 0 1807 1884
Size: 1807 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 369 401 1838 1870 1884
Size: 369 32 1437 32 14
Fragments arranged by size:
1437 369 32 32 14
HindIII A'AGCT_T
Cuts at: 0 788 1460 1544 1884
Size: 788 672 84 340
Fragments arranged by size:
788 672 340 84
MfeI C'AATT_G
Cuts at: 0 1299 1719 1884
Size: 1299 420 165
Fragments arranged by size:
1299 420 165
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 1884
Size: 308 1576
MscI TGG'CCA
Cuts at: 0 1641 1884
Size: 1641 243
MslI CAynn'nnrTG
Cuts at: 0 516 768 852 936 1246 1884
Size: 516 252 84 84 310 638
Fragments arranged by size:
638 516 310 252 84 84
NdeI CA'TA_TG
Cuts at: 0 905 1325 1493 1661 1884
Size: 905 420 168 168 223
Fragments arranged by size:
905 420 223 168 168
NsiI A_TGCA'T
Cuts at: 0 461 569 1884
Size: 461 108 1315
Fragments arranged by size:
1315 461 108
NspI r_CATG'y
Cuts at: 0 571 655 1411 1579 1747 1884
Size: 571 84 756 168 168 137
Fragments arranged by size:
756 571 168 168 137 84
PciI A'CATG_T
Cuts at: 0 1407 1575 1743 1884
Size: 1407 168 168 141
Fragments arranged by size:
1407 168 168 141
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 1884
Size: 106 32 1746
Fragments arranged by size:
1746 106 32
PpuMI rG'GwC_Cy
Cuts at: 0 78 1884
Size: 78 1806
SfcI C'TryA_G
Cuts at: 0 84 540 1592 1760 1884
Size: 84 456 1052 168 124
Fragments arranged by size:
1052 456 168 124 84
SmlI C'TyrA_G
Cuts at: 0 475 720 1734 1884
Size: 475 245 1014 150
Fragments arranged by size:
1014 475 245 150
SpeI A'CTAG_T
Cuts at: 0 730 1884
Size: 730 1154
StuI AGG'CCT
Cuts at: 0 538 958 1884
Size: 538 420 926
Fragments arranged by size:
926 538 420
StyI C'CwwG_G
Cuts at: 0 197 1884
Size: 197 1687
TatI w'GTAC_w
Cuts at: 0 395 1884
Size: 395 1489
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1155 1187 1210 1242 1884
Size: 1155 32 23 32 642
Fragments arranged by size:
1155 642 32 32 23
XmnI GAAnn'nnTTC
Cuts at: 0 231 790 1042 1079 1126 1210 1294 1378
Size: 231 559 252 37 47 84 84 84
Cuts at: 1378 1462 1546 1630 1714 1884
Size: 84 84 84 84 170
Fragments arranged by size:
559 252 231 170 84 84 84 84
84 84 84 47 37
Enzymes that do cut:
AclI AcuI AflII AflIII AlwNI ApaLI ApoI BbsI
BbvCI BclI BfrBI BglII Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BsgI
BsiHKAI BsiWI BsmI Bsp1286I BsrDI BsrGI BstAPI BstBI
BstXI BstYI BtgZI BtsI CspCI DrdI EaeI Eco57MI
EcoO109I EcoRI Hin4I HindIII MfeI MmeI MscI MslI
NdeI NsiI NspI PciI PpiI PpuMI SfcI SmlI
SpeI StuI StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AgeI AhdI AleI
AlfI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BcgI BciVI BglI
BlpI BmgBI BmtI BsaBI BsaHI BseYI BsiEI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstEII
BstZ17I Bsu36I BtgI ClaI DraI DraIII EagI EarI
EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MluI MspA1I NaeI
NarI NcoI NgoMIV NheI NotI NruI PacI PasI
PflMI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI SspI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI ZraI