(Linear) (Six-base) MAPSORT of: KIBB8346.seq  Check: 8359  from: 1  to: 381



KIBB8346.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AflIII A'CryG_T

Cuts at:      0     101     381 
   Size:        101     280
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     164     196     381 
   Size:        164      32     185

  Fragments arranged by size: 

                 185     164      32
 
ApoI r'AATT_y

Cuts at:      0     124     163     381 
   Size:        124      39     218

  Fragments arranged by size: 

                 218     124      39
 
AvaI C'yCGr_G

Cuts at:      0     230     381 
   Size:        230     151
 
BglII A'GATC_T

Cuts at:      0     234     309     381 
   Size:        234      75      72

  Fragments arranged by size: 

                 234      75      72
 
BsaBI GATnn'nnATC

Cuts at:      0      83     381 
   Size:         83     298
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     160     381 
   Size:        160     221
 
BspHI T'CATG_A

Cuts at:      0      72     381 
   Size:         72     309
 
BstYI r'GATC_y

Cuts at:      0       6     234     309     381 
   Size:          6     228      75      72

  Fragments arranged by size: 

                 228      75      72       6
 
EarI CTCTTCn'nnn_

Cuts at:      0     137     191     200     303     381 
   Size:        137      54       9     103      78

  Fragments arranged by size: 

                 137     103      78      54       9
 
EcoRI G'AATT_C

Cuts at:      0     124     381 
   Size:        124     257
 
MslI CAynn'nnrTG

Cuts at:      0      71      77     106     381 
   Size:         71       6      29     275

  Fragments arranged by size: 

                 275      71      29       6
 
NspI r_CATG'y

Cuts at:      0     105     381 
   Size:        105     276
 
PciI A'CATG_T

Cuts at:      0     101     381 
   Size:        101     280
 
SapI GCTCTTCn'nnn_

Cuts at:      0     200     303     381 
   Size:        200     103      78

  Fragments arranged by size: 

                 200     103      78
 
ScaI AGT'ACT

Cuts at:      0      24     381 
   Size:         24     357
 
SmlI C'TyrA_G

Cuts at:      0     230     381 
   Size:        230     151
 
SspI AAT'ATT

Cuts at:      0     378     381 
   Size:        378       3
 
TatI w'GTAC_w

Cuts at:      0      22      98     381 
   Size:         22      76     283

  Fragments arranged by size: 

                 283      76      22
 
XhoI C'TCGA_G

Cuts at:      0     230     381 
   Size:        230     151
 

 Enzymes that do cut:

   AflIII     AloI     ApoI     AvaI    BglII    BsaBI    BseRI    BspHI
    BstYI     EarI    EcoRI     MslI     NspI     PciI     SapI     ScaI
     SmlI     SspI     TatI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI