Restriction map for
pF1KB8354
(Linear) (Six-base) MAPSORT of: KIBB8354.seq Check: 6890 from: 1 to: 1371
KIBB8354.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AccI GT'mk_AC
Cuts at: 0 830 1371
Size: 830 541
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1003 1283 1371
Size: 1003 280 88
Fragments arranged by size:
1003 280 88
AflII C'TTAA_G
Cuts at: 0 739 1371
Size: 739 632
AflIII A'CryG_T
Cuts at: 0 1232 1371
Size: 1232 139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 868 1371
Size: 868 503
AlwNI CAG_nnn'CTG
Cuts at: 0 49 1371
Size: 49 1322
ApoI r'AATT_y
Cuts at: 0 362 694 1371
Size: 362 332 677
Fragments arranged by size:
677 362 332
BanI G'GyrC_C
Cuts at: 0 16 1371
Size: 16 1355
BanII G_rGCy'C
Cuts at: 0 401 1371
Size: 401 970
BbsI GAAGACnn'nnnn_
Cuts at: 0 587 1300 1371
Size: 587 713 71
Fragments arranged by size:
713 587 71
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 323 357 1371
Size: 323 34 1014
Fragments arranged by size:
1014 323 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 771 1371
Size: 771 600
BfrBI ATG'CAT
Cuts at: 0 334 1371
Size: 334 1037
BglII A'GATC_T
Cuts at: 0 1169 1371
Size: 1169 202
BlpI GC'TnA_GC
Cuts at: 0 56 716 1371
Size: 56 660 655
Fragments arranged by size:
660 655 56
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 1371
Size: 637 734
BsaAI yAC'GTr
Cuts at: 0 1368 1371
Size: 1368 3
BsaWI w'CCGG_w
Cuts at: 0 446 1371
Size: 446 925
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 140 170 485 515 1371
Size: 140 30 315 30 856
Fragments arranged by size:
856 315 140 30 30
BseYI C'CCAG_C
Cuts at: 0 1079 1371
Size: 1079 292
BsiHKAI G_wGCw'C
Cuts at: 0 401 1202 1371
Size: 401 801 169
Fragments arranged by size:
801 401 169
BsmI GAATG_Cn'
Cuts at: 0 378 559 1018 1371
Size: 378 181 459 353
Fragments arranged by size:
459 378 353 181
Bsp1286I G_dGCh'C
Cuts at: 0 401 1202 1371
Size: 401 801 169
Fragments arranged by size:
801 401 169
BspEI T'CCGG_A
Cuts at: 0 446 1371
Size: 446 925
BspHI T'CATG_A
Cuts at: 0 210 1088 1371
Size: 210 878 283
Fragments arranged by size:
878 283 210
BsrBI CCG'CTC
Cuts at: 0 1209 1371
Size: 1209 162
BsrDI GCAATG_nn'
Cuts at: 0 1289 1346 1371
Size: 1289 57 25
Fragments arranged by size:
1289 57 25
BstBI TT'CG_AA
Cuts at: 0 749 1371
Size: 749 622
BstYI r'GATC_y
Cuts at: 0 1169 1371
Size: 1169 202
BstZ17I GTA'TAC
Cuts at: 0 831 1371
Size: 831 540
Bsu36I CC'TnA_GG
Cuts at: 0 1358 1371
Size: 1358 13
EaeI y'GGCC_r
Cuts at: 0 968 1371
Size: 968 403
EarI CTCTTCn'nnn_
Cuts at: 0 1262 1343 1371
Size: 1262 81 28
Fragments arranged by size:
1262 81 28
EcoICRI GAG'CTC
Cuts at: 0 399 1371
Size: 399 972
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 1003 1283 1371
Size: 637 366 280 88
Fragments arranged by size:
637 366 280 88
EcoO109I rG'GnC_Cy
Cuts at: 0 1026 1371
Size: 1026 345
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 754 1134 1371
Size: 754 380 237
Fragments arranged by size:
754 380 237
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 61 93 428 460 1087 1119 1154 1186
Size: 61 32 335 32 627 32 35 32
Cuts at: 1186 1371
Size: 185
Fragments arranged by size:
627 335 185 61 35 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 929 985 1371
Size: 929 56 386
Fragments arranged by size:
929 386 56
MfeI C'AATT_G
Cuts at: 0 596 1371
Size: 596 775
MscI TGG'CCA
Cuts at: 0 970 1371
Size: 970 401
MslI CAynn'nnrTG
Cuts at: 0 277 1371
Size: 277 1094
MspA1I CmG'CkG
Cuts at: 0 524 630 1083 1371
Size: 524 106 453 288
Fragments arranged by size:
524 453 288 106
NdeI CA'TA_TG
Cuts at: 0 336 1371
Size: 336 1035
NsiI A_TGCA'T
Cuts at: 0 336 1371
Size: 336 1035
NspI r_CATG'y
Cuts at: 0 855 1236 1371
Size: 855 381 135
Fragments arranged by size:
855 381 135
PciI A'CATG_T
Cuts at: 0 1232 1371
Size: 1232 139
PflMI CCAn_nnn'nTGG
Cuts at: 0 667 1371
Size: 667 704
PstI C_TGCA'G
Cuts at: 0 550 1169 1371
Size: 550 619 202
Fragments arranged by size:
619 550 202
PvuII CAG'CTG
Cuts at: 0 1083 1371
Size: 1083 288
SacI G_AGCT'C
Cuts at: 0 401 1371
Size: 401 970
SfcI C'TryA_G
Cuts at: 0 546 1165 1371
Size: 546 619 206
Fragments arranged by size:
619 546 206
SmlI C'TyrA_G
Cuts at: 0 739 1371
Size: 739 632
StyI C'CwwG_G
Cuts at: 0 1029 1109 1193 1262 1371
Size: 1029 80 84 69 109
Fragments arranged by size:
1029 109 84 80 69
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 789 1281 1371
Size: 789 492 90
Fragments arranged by size:
789 492 90
XmnI GAAnn'nnTTC
Cuts at: 0 941 1371
Size: 941 430
Enzymes that do cut:
AccI AcuI AflII AflIII AlfI AlwNI ApoI BanI
BanII BbsI BcgI BciVI BfrBI BglII BlpI BpmI
BsaAI BsaWI BsaXI BseYI BsiHKAI BsmI Bsp1286I BspEI
BspHI BsrBI BsrDI BstBI BstYI BstZ17I Bsu36I EaeI
EarI EcoICRI Eco57MI EcoO109I FalI Hin4I HindIII MfeI
MscI MslI MspA1I NdeI NsiI NspI PciI PflMI
PstI PvuII SacI SfcI SmlI StyI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BclI BglI Bme1580I BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaHI
BseRI BsgI BsiEI BsiWI BsmBI BspMI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstXI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MluI MmeI NaeI NarI NcoI NgoMIV
NheI NotI NruI PacI PasI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI ZraI