(Linear) (Six-base) MAPSORT of: KIBB8361.seq  Check: 2875  from: 1  to: 432



KIBB8361.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

ApoI r'AATT_y

Cuts at:      0      60      69     244     432 
   Size:         60       9     175     188

  Fragments arranged by size: 

                 188     175      60       9
 
AvaI C'yCGr_G

Cuts at:      0     270     432 
   Size:        270     162
 
BamHI G'GATC_C

Cuts at:      0     160     432 
   Size:        160     272
 
BanI G'GyrC_C

Cuts at:      0     238     432 
   Size:        238     194
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      10     432 
   Size:         10     422
 
BclI T'GATC_A

Cuts at:      0      26      83     432 
   Size:         26      57     349

  Fragments arranged by size: 

                 349      57      26
 
BglI GCCn_nnn'nGGC

Cuts at:      0     143     432 
   Size:        143     289
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     432 
   Size:        241     191
 
BsaBI GATnn'nnATC

Cuts at:      0      31     432 
   Size:         31     401
 
BsiHKAI G_wGCw'C

Cuts at:      0     421     432 
   Size:        421      11
 
Bsp1286I G_dGCh'C

Cuts at:      0     421     432 
   Size:        421      11
 
BspHI T'CATG_A

Cuts at:      0      29     198     432 
   Size:         29     169     234

  Fragments arranged by size: 

                 234     169      29
 
BstYI r'GATC_y

Cuts at:      0     160     432 
   Size:        160     272
 
BtgI C'CryG_G

Cuts at:      0      54     432 
   Size:         54     378
 
BtsI GCAGTG_nn'

Cuts at:      0      29     405     432 
   Size:         29     376      27

  Fragments arranged by size: 

                 376      29      27
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     333     368     432 
   Size:        333      35      64

  Fragments arranged by size: 

                 333      64      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     432 
   Size:        241     191
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      46     432 
   Size:         46     386
 
HincII GTy'rAC

Cuts at:      0      92     316     432 
   Size:         92     224     116

  Fragments arranged by size: 

                 224     116      92
 
NcoI C'CATG_G

Cuts at:      0      54     432 
   Size:         54     378
 
PstI C_TGCA'G

Cuts at:      0      50     310     412     432 
   Size:         50     260     102      20

  Fragments arranged by size: 

                 260     102      50      20
 
ScaI AGT'ACT

Cuts at:      0     391     432 
   Size:        391      41
 
SfcI C'TryA_G

Cuts at:      0      46     306     408     432 
   Size:         46     260     102      24

  Fragments arranged by size: 

                 260     102      46      24
 
StyI C'CwwG_G

Cuts at:      0      54     167     432 
   Size:         54     113     265

  Fragments arranged by size: 

                 265     113      54
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     139     432 
   Size:        139     293
 
TatI w'GTAC_w

Cuts at:      0     389     432 
   Size:        389      43
 

 Enzymes that do cut:

     ApoI     AvaI    BamHI     BanI     BbsI     BclI     BglI     BpmI
    BsaBI  BsiHKAI Bsp1286I    BspHI    BstYI     BtgI     BtsI    CspCI
  Eco57MI    EcoNI   HincII     NcoI     PstI     ScaI     SfcI     StyI
    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BanII     BbeI
    BbvCI     BcgI    BciVI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     ClaI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI