Restriction map for
pF1KB8372
(Linear) (Six-base) MAPSORT of: KIBB8372.seq Check: 5905 from: 1 to: 495
KIBB8372.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AccI GT'mk_AC
Cuts at: 0 146 495
Size: 146 349
AflII C'TTAA_G
Cuts at: 0 417 495
Size: 417 78
ApoI r'AATT_y
Cuts at: 0 13 495
Size: 13 482
AseI AT'TA_AT
Cuts at: 0 479 495
Size: 479 16
AvaI C'yCGr_G
Cuts at: 0 282 495
Size: 282 213
BamHI G'GATC_C
Cuts at: 0 408 495
Size: 408 87
BanI G'GyrC_C
Cuts at: 0 343 495
Size: 343 152
BanII G_rGCy'C
Cuts at: 0 302 495
Size: 302 193
BbeI G_GCGC'C
Cuts at: 0 347 495
Size: 347 148
BbsI GAAGACnn'nnnn_
Cuts at: 0 40 495
Size: 40 455
BlpI GC'TnA_GC
Cuts at: 0 79 495
Size: 79 416
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 33 495
Size: 33 462
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 88 495
Size: 44 44 407
Fragments arranged by size:
407 44 44
BsaI GGTCTCn'nnnn_
Cuts at: 0 307 495
Size: 307 188
BsaHI Gr'CG_yC
Cuts at: 0 344 495
Size: 344 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 495
Size: 349 146
BsiHKAI G_wGCw'C
Cuts at: 0 80 302 495
Size: 80 222 193
Fragments arranged by size:
222 193 80
Bsp1286I G_dGCh'C
Cuts at: 0 80 302 495
Size: 80 222 193
Fragments arranged by size:
222 193 80
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 371 495
Size: 371 124
BsrFI r'CCGG_y
Cuts at: 0 287 495
Size: 287 208
BstAPI GCAn_nnn'nTGC
Cuts at: 0 372 495
Size: 372 123
BstYI r'GATC_y
Cuts at: 0 215 408 495
Size: 215 193 87
Fragments arranged by size:
215 193 87
BtgI C'CryG_G
Cuts at: 0 329 495
Size: 329 166
BtsI GCAGTG_nn'
Cuts at: 0 366 495
Size: 366 129
DraIII CAC_nnn'GTG
Cuts at: 0 372 495
Size: 372 123
EaeI y'GGCC_r
Cuts at: 0 128 495
Size: 128 367
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 196 310 495
Size: 196 114 185
Fragments arranged by size:
196 185 114
EcoICRI GAG'CTC
Cuts at: 0 300 495
Size: 300 195
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 88 495
Size: 44 44 407
Fragments arranged by size:
407 44 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 232 495
Size: 232 263
EcoRI G'AATT_C
Cuts at: 0 13 495
Size: 13 482
HaeII r_GCGC'y
Cuts at: 0 347 495
Size: 347 148
HincII GTy'rAC
Cuts at: 0 147 424 495
Size: 147 277 71
Fragments arranged by size:
277 147 71
KasI G'GCGC_C
Cuts at: 0 343 495
Size: 343 152
MslI CAynn'nnrTG
Cuts at: 0 399 495
Size: 399 96
MspA1I CmG'CkG
Cuts at: 0 331 495
Size: 331 164
NaeI GCC'GGC
Cuts at: 0 289 495
Size: 289 206
NarI GG'CG_CC
Cuts at: 0 344 495
Size: 344 151
NgoMIV G'CCGG_C
Cuts at: 0 287 495
Size: 287 208
PstI C_TGCA'G
Cuts at: 0 215 495
Size: 215 280
SacI G_AGCT'C
Cuts at: 0 302 495
Size: 302 193
SacII CC_GC'GG
Cuts at: 0 332 495
Size: 332 163
SalI G'TCGA_C
Cuts at: 0 145 495
Size: 145 350
SfcI C'TryA_G
Cuts at: 0 211 495
Size: 211 284
SfoI GGC'GCC
Cuts at: 0 345 495
Size: 345 150
SmaI CCC'GGG
Cuts at: 0 284 495
Size: 284 211
SmlI C'TyrA_G
Cuts at: 0 417 495
Size: 417 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 52 331 495
Size: 52 279 164
Fragments arranged by size:
279 164 52
XmaI C'CCGG_G
Cuts at: 0 282 495
Size: 282 213
Enzymes that do cut:
AccI AflII ApoI AseI AvaI BamHI BanI BanII
BbeI BbsI BlpI BplI BpmI BsaI BsaHI BsgI
BsiHKAI Bsp1286I BspMI BsrFI BstAPI BstYI BtgI BtsI
DraIII EaeI EciI EcoICRI Eco57MI EcoNI EcoRI HaeII
HincII KasI MslI MspA1I NaeI NarI NgoMIV PstI
SacI SacII SalI SfcI SfoI SmaI SmlI TaqII
XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII Bme1580I BmgBI BmrI BmtI Bpu10I BpuEI
BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EagI EarI EcoO109I EcoRV FalI
FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI
MluI MmeI MscI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmnI ZraI