Restriction map for
pF1KB8376
(Linear) (Six-base) MAPSORT of: KIBB8376.seq Check: 8308 from: 1 to: 1878
KIBB8376.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 582 1878
Size: 582 1296
AatII G_ACGT'C
Cuts at: 0 707 1878
Size: 707 1171
AleI CACnn'nnGTG
Cuts at: 0 65 460 586 1878
Size: 65 395 126 1292
Fragments arranged by size:
1292 395 126 65
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 322 604 1878
Size: 322 282 1274
Fragments arranged by size:
1274 322 282
AlwNI CAG_nnn'CTG
Cuts at: 0 1095 1290 1878
Size: 1095 195 588
Fragments arranged by size:
1095 588 195
AvaI C'yCGr_G
Cuts at: 0 50 325 1150 1271 1878
Size: 50 275 825 121 607
Fragments arranged by size:
825 607 275 121 50
BanI G'GyrC_C
Cuts at: 0 67 432 480 580 1483 1552 1698 1724
Size: 67 365 48 100 903 69 146 26
Cuts at: 1724 1878
Size: 154
Fragments arranged by size:
903 365 154 146 100 69 67 48
26
BanII G_rGCy'C
Cuts at: 0 548 763 959 1518 1636 1769 1878
Size: 548 215 196 559 118 133 109
Fragments arranged by size:
559 548 215 196 133 118 109
BbeI G_GCGC'C
Cuts at: 0 1487 1556 1728 1878
Size: 1487 69 172 150
Fragments arranged by size:
1487 172 150 69
BbsI GAAGACnn'nnnn_
Cuts at: 0 210 902 1878
Size: 210 692 976
Fragments arranged by size:
976 692 210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1383 1417 1878
Size: 1383 34 461
Fragments arranged by size:
1383 461 34
BglI GCCn_nnn'nGGC
Cuts at: 0 916 1878
Size: 916 962
BlpI GC'TnA_GC
Cuts at: 0 310 514 1878
Size: 310 204 1364
Fragments arranged by size:
1364 310 204
Bme1580I G_kGCm'C
Cuts at: 0 437 583 617 1701 1878
Size: 437 146 34 1084 177
Fragments arranged by size:
1084 437 177 146 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 72 1878
Size: 72 1806
BmtI G_CTAG'C
Cuts at: 0 1852 1878
Size: 1852 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 753 1044 1808 1878
Size: 753 291 764 70
Fragments arranged by size:
764 753 291 70
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 895 1150 1270 1516 1878
Size: 889 6 255 120 246 362
Fragments arranged by size:
889 362 255 246 120 6
Bpu10I CC'TnA_GC
Cuts at: 0 509 1783 1878
Size: 509 1274 95
Fragments arranged by size:
1274 509 95
BsaI GGTCTCn'nnnn_
Cuts at: 0 1032 1878
Size: 1032 846
BsaAI yAC'GTr
Cuts at: 0 107 768 1878
Size: 107 661 1110
Fragments arranged by size:
1110 661 107
BsaBI GATnn'nnATC
Cuts at: 0 1582 1878
Size: 1582 296
BsaHI Gr'CG_yC
Cuts at: 0 704 902 1484 1553 1725 1878
Size: 704 198 582 69 172 153
Fragments arranged by size:
704 582 198 172 153 69
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1366 1396 1878
Size: 1366 30 482
Fragments arranged by size:
1366 482 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 729 892 1234 1755 1858 1878
Size: 729 163 342 521 103 20
Fragments arranged by size:
729 521 342 163 103 20
BseYI C'CCAG_C
Cuts at: 0 330 522 537 555 642 1636 1749 1878
Size: 330 192 15 18 87 994 113 129
Fragments arranged by size:
994 330 192 129 113 87 18 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 589 610 1303 1446 1878
Size: 282 307 21 693 143 432
Fragments arranged by size:
693 432 307 282 143 21
BsiEI CG_ry'CG
Cuts at: 0 1142 1878
Size: 1142 736
BsiHKAI G_wGCw'C
Cuts at: 0 395 1042 1244 1409 1878
Size: 395 647 202 165 469
Fragments arranged by size:
647 469 395 202 165
BsmI GAATG_Cn'
Cuts at: 0 930 1878
Size: 930 948
BsmBI CGTCTCn'nnnn_
Cuts at: 0 117 1015 1878
Size: 117 898 863
Fragments arranged by size:
898 863 117
Bsp1286I G_dGCh'C
Cuts at: 0 395 437 548 583 617 763 959 1042
Size: 395 42 111 35 34 146 196 83
Cuts at: 1042 1244 1409 1518 1636 1701 1769 1878
Size: 202 165 109 118 65 68 109
Fragments arranged by size:
395 202 196 165 146 118 111 109
109 83 68 65 42 35 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 582 1878
Size: 582 1296
BsrBI CCG'CTC
Cuts at: 0 1371 1878
Size: 1371 507
BsrDI GCAATG_nn'
Cuts at: 0 1353 1878
Size: 1353 525
BsrFI r'CCGG_y
Cuts at: 0 560 1137 1878
Size: 560 577 741
Fragments arranged by size:
741 577 560
BssSI C'ACGA_G
Cuts at: 0 244 529 618 1402 1878
Size: 244 285 89 784 476
Fragments arranged by size:
784 476 285 244 89
BstAPI GCAn_nnn'nTGC
Cuts at: 0 612 1878
Size: 612 1266
BstBI TT'CG_AA
Cuts at: 0 98 1878
Size: 98 1780
BstEII G'GTnAC_C
Cuts at: 0 882 1878
Size: 882 996
BstXI CCAn_nnnn'nTGG
Cuts at: 0 429 1494 1878
Size: 429 1065 384
Fragments arranged by size:
1065 429 384
BstYI r'GATC_y
Cuts at: 0 1091 1732 1878
Size: 1091 641 146
Fragments arranged by size:
1091 641 146
BtgI C'CryG_G
Cuts at: 0 351 950 1100 1322 1556 1878
Size: 351 599 150 222 234 322
Fragments arranged by size:
599 351 322 234 222 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1325 1878
Size: 1325 553
BtsI GCAGTG_nn'
Cuts at: 0 16 598 1257 1291 1878
Size: 16 582 659 34 587
Fragments arranged by size:
659 587 582 34 16
DraIII CAC_nnn'GTG
Cuts at: 0 612 1878
Size: 612 1266
DrdI GACnn_nn'nnGTC
Cuts at: 0 832 1878
Size: 832 1046
EaeI y'GGCC_r
Cuts at: 0 633 851 917 933 1007 1139 1194 1878
Size: 633 218 66 16 74 132 55 684
Fragments arranged by size:
684 633 218 132 74 66 55 16
EagI C'GGCC_G
Cuts at: 0 1139 1878
Size: 1139 739
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 895 1150 1270 1516 1878
Size: 889 6 255 120 246 362
Fragments arranged by size:
889 362 255 246 120 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 841 1878
Size: 841 1037
EcoO109I rG'GnC_Cy
Cuts at: 0 279 357 1443 1878
Size: 279 78 1086 435
Fragments arranged by size:
1086 435 279 78
EcoRV GAT'ATC
Cuts at: 0 408 1878
Size: 408 1470
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 363 1878
Size: 363 1515
HaeII r_GCGC'y
Cuts at: 0 1487 1556 1728 1878
Size: 1487 69 172 150
Fragments arranged by size:
1487 172 150 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1324 1356 1878
Size: 1324 32 522
Fragments arranged by size:
1324 522 32
HincII GTy'rAC
Cuts at: 0 414 1878
Size: 414 1464
KasI G'GCGC_C
Cuts at: 0 1483 1552 1724 1878
Size: 1483 69 172 154
Fragments arranged by size:
1483 172 154 69
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1331 1878
Size: 1331 547
MscI TGG'CCA
Cuts at: 0 935 1196 1878
Size: 935 261 682
Fragments arranged by size:
935 682 261
MslI CAynn'nnrTG
Cuts at: 0 65 430 460 586 1878
Size: 65 365 30 126 1292
Fragments arranged by size:
1292 365 126 65 30
MspA1I CmG'CkG
Cuts at: 0 157 473 550 807 1128 1397 1878
Size: 157 316 77 257 321 269 481
Fragments arranged by size:
481 321 316 269 257 157 77
NaeI GCC'GGC
Cuts at: 0 562 1139 1878
Size: 562 577 739
Fragments arranged by size:
739 577 562
NarI GG'CG_CC
Cuts at: 0 1484 1553 1725 1878
Size: 1484 69 172 153
Fragments arranged by size:
1484 172 153 69
NgoMIV G'CCGG_C
Cuts at: 0 560 1137 1878
Size: 560 577 741
Fragments arranged by size:
741 577 560
NheI G'CTAG_C
Cuts at: 0 1848 1878
Size: 1848 30
PflMI CCAn_nnn'nTGG
Cuts at: 0 642 1878
Size: 642 1236
PfoI T'CCnGG_A
Cuts at: 0 137 401 839 866 1247 1878
Size: 137 264 438 27 381 631
Fragments arranged by size:
631 438 381 264 137 27
PmlI CAC'GTG
Cuts at: 0 107 768 1878
Size: 107 661 1110
Fragments arranged by size:
1110 661 107
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1360 1392 1878
Size: 1360 32 486
Fragments arranged by size:
1360 486 32
PpuMI rG'GwC_Cy
Cuts at: 0 279 357 1878
Size: 279 78 1521
Fragments arranged by size:
1521 279 78
PstI C_TGCA'G
Cuts at: 0 680 1127 1878
Size: 680 447 751
Fragments arranged by size:
751 680 447
PvuII CAG'CTG
Cuts at: 0 473 1128 1878
Size: 473 655 750
Fragments arranged by size:
750 655 473
ScaI AGT'ACT
Cuts at: 0 343 1168 1878
Size: 343 825 710
Fragments arranged by size:
825 710 343
SfcI C'TryA_G
Cuts at: 0 676 1123 1878
Size: 676 447 755
Fragments arranged by size:
755 676 447
SfoI GGC'GCC
Cuts at: 0 1485 1554 1726 1878
Size: 1485 69 172 152
Fragments arranged by size:
1485 172 152 69
StuI AGG'CCT
Cuts at: 0 655 1878
Size: 655 1223
StyI C'CwwG_G
Cuts at: 0 1820 1878
Size: 1820 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 247 576 1878
Size: 247 329 1302
Fragments arranged by size:
1302 329 247
TatI w'GTAC_w
Cuts at: 0 341 377 1166 1382 1878
Size: 341 36 789 216 496
Fragments arranged by size:
789 496 341 216 36
Tth111I GACn'n_nGTC
Cuts at: 0 901 1878
Size: 901 977
ZraI GAC'GTC
Cuts at: 0 705 1878
Size: 705 1173
Enzymes that do cut:
AarI AatII AleI AlfI AlwNI AvaI BanI BanII
BbeI BbsI BcgI BglI BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI
EagI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NheI PflMI PfoI PmlI PpiI PpuMI PstI
PvuII ScaI SfcI SfoI StuI StyI TaqII TatI
Tth111I ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglII
BmgBI BpuEI BsaWI BsiWI BspEI BspHI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI DraI EarI EciI EcoICRI
EcoRI FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI NcoI NdeI NotI NruI NsiI NspI PacI
PasI PciI PmeI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TstI XbaI XcmI XhoI
XmaI XmnI