Restriction map for
pF1KB8379
(Linear) (Six-base) MAPSORT of: KIBB8379.seq Check: 8301 from: 1 to: 1842
KIBB8379.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 737 1056 1842
Size: 737 319 786
Fragments arranged by size:
786 737 319
AccI GT'mk_AC
Cuts at: 0 272 1367 1842
Size: 272 1095 475
Fragments arranged by size:
1095 475 272
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 1113 1842
Size: 178 935 729
Fragments arranged by size:
935 729 178
AflIII A'CryG_T
Cuts at: 0 392 485 1842
Size: 392 93 1357
Fragments arranged by size:
1357 392 93
AhdI GACnn_n'nnGTC
Cuts at: 0 1143 1842
Size: 1143 699
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 745 797 1842
Size: 745 52 1045
Fragments arranged by size:
1045 745 52
AlwNI CAG_nnn'CTG
Cuts at: 0 18 624 933 1071 1518 1842
Size: 18 606 309 138 447 324
Fragments arranged by size:
606 447 324 309 138 18
ApaI G_GGCC'C
Cuts at: 0 175 1122 1842
Size: 175 947 720
Fragments arranged by size:
947 720 175
ApaLI G'TGCA_C
Cuts at: 0 1786 1842
Size: 1786 56
AvaI C'yCGr_G
Cuts at: 0 166 594 1255 1361 1568 1842
Size: 166 428 661 106 207 274
Fragments arranged by size:
661 428 274 207 166 106
BamHI G'GATC_C
Cuts at: 0 1460 1554 1842
Size: 1460 94 288
Fragments arranged by size:
1460 288 94
BanI G'GyrC_C
Cuts at: 0 337 781 1357 1425 1842
Size: 337 444 576 68 417
Fragments arranged by size:
576 444 417 337 68
BanII G_rGCy'C
Cuts at: 0 175 248 1099 1122 1568 1614 1777 1842
Size: 175 73 851 23 446 46 163 65
Fragments arranged by size:
851 446 175 163 73 65 46 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 166 966 1842
Size: 166 800 876
Fragments arranged by size:
876 800 166
BbvCI CC'TCA_GC
Cuts at: 0 92 1842
Size: 92 1750
BglI GCCn_nnn'nGGC
Cuts at: 0 1333 1842
Size: 1333 509
BlpI GC'TnA_GC
Cuts at: 0 367 1613 1711 1842
Size: 367 1246 98 131
Fragments arranged by size:
1246 367 131 98
Bme1580I G_kGCm'C
Cuts at: 0 175 340 786 1122 1321 1362 1430 1790
Size: 175 165 446 336 199 41 68 360
Cuts at: 1790 1842
Size: 52
Fragments arranged by size:
446 360 336 199 175 165 68 52
41
BmgBI CAC'GTC
Cuts at: 0 486 1842
Size: 486 1356
BmrI ACTGGGnnnn_n'
Cuts at: 0 1539 1842
Size: 1539 303
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1030 1842
Size: 1030 812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 851 1666 1791 1842
Size: 851 815 125 51
Fragments arranged by size:
851 815 125 51
Bpu10I CC'TnA_GC
Cuts at: 0 92 1285 1842
Size: 92 1193 557
Fragments arranged by size:
1193 557 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 947 1842
Size: 947 895
BsaI GGTCTCn'nnnn_
Cuts at: 0 613 1565 1842
Size: 613 952 277
Fragments arranged by size:
952 613 277
BsaAI yAC'GTr
Cuts at: 0 954 1842
Size: 954 888
BsaWI w'CCGG_w
Cuts at: 0 240 977 1842
Size: 240 737 865
Fragments arranged by size:
865 737 240
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 38 68 1732 1762 1842
Size: 38 30 1664 30 80
Fragments arranged by size:
1664 80 38 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 49 52 95 465 1510 1842
Size: 46 3 3 43 370 1045 332
Fragments arranged by size:
1045 370 332 46 43 3 3
BseYI C'CCAG_C
Cuts at: 0 742 1328 1336 1511 1842
Size: 742 586 8 175 331
Fragments arranged by size:
742 586 331 175 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 661 1842
Size: 7 654 1181
Fragments arranged by size:
1181 654 7
BsiEI CG_ry'CG
Cuts at: 0 1642 1842
Size: 1642 200
BsiHKAI G_wGCw'C
Cuts at: 0 1777 1790 1842
Size: 1777 13 52
Fragments arranged by size:
1777 52 13
BsmI GAATG_Cn'
Cuts at: 0 149 1842
Size: 149 1693
Bsp1286I G_dGCh'C
Cuts at: 0 175 248 340 786 1099 1122 1321 1362
Size: 175 73 92 446 313 23 199 41
Cuts at: 1362 1430 1568 1614 1777 1790 1842
Size: 68 138 46 163 13 52
Fragments arranged by size:
446 313 199 175 163 138 92 73
68 52 46 41 23 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 737 918 1056 1643 1842
Size: 737 181 138 587 199
Fragments arranged by size:
737 587 199 181 138
BsrBI CCG'CTC
Cuts at: 0 779 1842
Size: 779 1063
BsrDI GCAATG_nn'
Cuts at: 0 606 1842
Size: 606 1236
BsrFI r'CCGG_y
Cuts at: 0 137 1842
Size: 137 1705
BssHII G'CGCG_C
Cuts at: 0 1665 1842
Size: 1665 177
BstAPI GCAn_nnn'nTGC
Cuts at: 0 18 639 957 1842
Size: 18 621 318 885
Fragments arranged by size:
885 621 318 18
BstEII G'GTnAC_C
Cuts at: 0 667 1842
Size: 667 1175
BstXI CCAn_nnnn'nTGG
Cuts at: 0 843 1336 1842
Size: 843 493 506
Fragments arranged by size:
843 506 493
BstYI r'GATC_y
Cuts at: 0 443 1460 1554 1842
Size: 443 1017 94 288
Fragments arranged by size:
1017 443 288 94
Bsu36I CC'TnA_GG
Cuts at: 0 1273 1842
Size: 1273 569
BtgI C'CryG_G
Cuts at: 0 215 803 1056 1322 1431 1842
Size: 215 588 253 266 109 411
Fragments arranged by size:
588 411 266 253 215 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1304 1842
Size: 1304 538
BtsI GCAGTG_nn'
Cuts at: 0 19 640 1842
Size: 19 621 1202
Fragments arranged by size:
1202 621 19
DraIII CAC_nnn'GTG
Cuts at: 0 855 1842
Size: 855 987
DrdI GACnn_nn'nnGTC
Cuts at: 0 1309 1842
Size: 1309 533
EaeI y'GGCC_r
Cuts at: 0 495 1172 1639 1842
Size: 495 677 467 203
Fragments arranged by size:
677 495 467 203
EagI C'GGCC_G
Cuts at: 0 1639 1842
Size: 1639 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1753 1842
Size: 1753 89
EcoICRI GAG'CTC
Cuts at: 0 1775 1842
Size: 1775 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 851 1113 1666 1791 1842
Size: 178 673 262 553 125 51
Fragments arranged by size:
673 553 262 178 125 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 260 1276 1842
Size: 260 1016 566
Fragments arranged by size:
1016 566 260
EcoO109I rG'GnC_Cy
Cuts at: 0 5 171 172 220 470 591 750 930
Size: 5 166 1 48 250 121 159 180
Cuts at: 930 1032 1118 1119 1260 1281 1581 1842
Size: 102 86 1 141 21 300 261
Fragments arranged by size:
300 261 250 180 166 159 141 121
102 86 48 21 5 1 1
HaeII r_GCGC'y
Cuts at: 0 1718 1746 1842
Size: 1718 28 96
Fragments arranged by size:
1718 96 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 661 693 1081 1113 1129 1161 1816 1842
Size: 661 32 388 32 16 32 655 26
Fragments arranged by size:
661 655 388 32 32 32 26 16
MslI CAynn'nnrTG
Cuts at: 0 343 1842
Size: 343 1499
MspA1I CmG'CkG
Cuts at: 0 843 1332 1644 1769 1842
Size: 843 489 312 125 73
Fragments arranged by size:
843 489 312 125 73
NaeI GCC'GGC
Cuts at: 0 139 1842
Size: 139 1703
NcoI C'CATG_G
Cuts at: 0 215 1842
Size: 215 1627
NgoMIV G'CCGG_C
Cuts at: 0 137 1842
Size: 137 1705
NotI GC'GGCC_GC
Cuts at: 0 1639 1842
Size: 1639 203
NspI r_CATG'y
Cuts at: 0 726 1842
Size: 726 1116
PasI CC'CwG_GG
Cuts at: 0 797 1353 1842
Size: 797 556 489
Fragments arranged by size:
797 556 489
PflMI CCAn_nnn'nTGG
Cuts at: 0 309 855 1842
Size: 309 546 987
Fragments arranged by size:
987 546 309
PfoI T'CCnGG_A
Cuts at: 0 611 1842
Size: 611 1231
PmlI CAC'GTG
Cuts at: 0 954 1842
Size: 954 888
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1432 1464 1842
Size: 1432 32 378
Fragments arranged by size:
1432 378 32
PpuMI rG'GwC_Cy
Cuts at: 0 220 930 1260 1842
Size: 220 710 330 582
Fragments arranged by size:
710 582 330 220
PspOMI G'GGCC_C
Cuts at: 0 171 1118 1842
Size: 171 947 724
Fragments arranged by size:
947 724 171
PstI C_TGCA'G
Cuts at: 0 635 776 1067 1842
Size: 635 141 291 775
Fragments arranged by size:
775 635 291 141
PvuII CAG'CTG
Cuts at: 0 843 1332 1842
Size: 843 489 510
Fragments arranged by size:
843 510 489
SacI G_AGCT'C
Cuts at: 0 1777 1842
Size: 1777 65
SanDI GG'GwC_CC
Cuts at: 0 220 1842
Size: 220 1622
SbfI CC_TGCA'GG
Cuts at: 0 1067 1842
Size: 1067 775
SfcI C'TryA_G
Cuts at: 0 462 631 772 1063 1812 1842
Size: 462 169 141 291 749 30
Fragments arranged by size:
749 462 291 169 141 30
SmaI CCC'GGG
Cuts at: 0 168 596 1363 1842
Size: 168 428 767 479
Fragments arranged by size:
767 479 428 168
SmlI C'TyrA_G
Cuts at: 0 962 1842
Size: 962 880
SphI G_CATG'C
Cuts at: 0 726 1842
Size: 726 1116
StuI AGG'CCT
Cuts at: 0 1675 1842
Size: 1675 167
StyI C'CwwG_G
Cuts at: 0 215 256 479 1577 1842
Size: 215 41 223 1098 265
Fragments arranged by size:
1098 265 223 215 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 64 228 627 1066 1842
Size: 64 164 399 439 776
Fragments arranged by size:
776 439 399 164 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 16 38 48 70 718 750 1378 1410
Size: 16 22 10 22 648 32 628 32
Cuts at: 1410 1539 1571 1732 1764 1842
Size: 129 32 161 32 78
Fragments arranged by size:
648 628 161 129 78 32 32 32
32 22 22 16 10
Tth111I GACn'n_nGTC
Cuts at: 0 224 1842
Size: 224 1618
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 810 1842
Size: 810 1032
XmaI C'CCGG_G
Cuts at: 0 166 594 1361 1842
Size: 166 428 767 481
Fragments arranged by size:
767 481 428 166
XmnI GAAnn'nnTTC
Cuts at: 0 1504 1693 1801 1842
Size: 1504 189 108 41
Fragments arranged by size:
1504 189 108 41
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlfI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbsI BbvCI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I MslI MspA1I NaeI NcoI NgoMIV
NotI NspI PasI PflMI PfoI PmlI PpiI PpuMI
PspOMI PstI PvuII SacI SanDI SbfI SfcI SmaI
SmlI SphI StuI StyI TaqII TstI Tth111I XcmI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AloI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BcgI
BciVI BclI BfrBI BglII BmtI BsaBI BsaHI BsiWI
BsmBI BspEI BspHI BsrGI BssSI BstBI BstZ17I ClaI
CspCI DraI EarI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NarI NdeI NheI NruI NsiI PacI
PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI TatI XbaI
XhoI ZraI