Restriction map for
pF1KB8381
(Linear) (Six-base) MAPSORT of: KIBB8381.seq Check: 7092 from: 1 to: 1506
KIBB8381.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 370 1506
Size: 34 336 1136
Fragments arranged by size:
1136 336 34
AlwNI CAG_nnn'CTG
Cuts at: 0 96 1506
Size: 96 1410
ApaLI G'TGCA_C
Cuts at: 0 565 1506
Size: 565 941
ApoI r'AATT_y
Cuts at: 0 287 443 1394 1506
Size: 287 156 951 112
Fragments arranged by size:
951 287 156 112
AseI AT'TA_AT
Cuts at: 0 332 1506
Size: 332 1174
AvaI C'yCGr_G
Cuts at: 0 841 1506
Size: 841 665
AvrII C'CTAG_G
Cuts at: 0 487 1506
Size: 487 1019
BanI G'GyrC_C
Cuts at: 0 106 618 1506
Size: 106 512 888
Fragments arranged by size:
888 512 106
BanII G_rGCy'C
Cuts at: 0 12 1506
Size: 12 1494
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1000 1034 1506
Size: 1000 34 472
Fragments arranged by size:
1000 472 34
BfrBI ATG'CAT
Cuts at: 0 1138 1506
Size: 1138 368
BlpI GC'TnA_GC
Cuts at: 0 998 1334 1506
Size: 998 336 172
Fragments arranged by size:
998 336 172
Bme1580I G_kGCm'C
Cuts at: 0 569 1506
Size: 569 937
BmrI ACTGGGnnnn_n'
Cuts at: 0 106 1506
Size: 106 1400
BmtI G_CTAG'C
Cuts at: 0 74 1506
Size: 74 1432
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 185 1506
Size: 185 1321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 438 455 938 1506
Size: 212 226 17 483 568
Fragments arranged by size:
568 483 226 212 17
BsaAI yAC'GTr
Cuts at: 0 851 1506
Size: 851 655
BsaBI GATnn'nnATC
Cuts at: 0 1099 1487 1506
Size: 1099 388 19
Fragments arranged by size:
1099 388 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 511 541 1506
Size: 511 30 965
Fragments arranged by size:
965 511 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 746 1506
Size: 76 670 760
Fragments arranged by size:
760 670 76
BsiHKAI G_wGCw'C
Cuts at: 0 569 680 1406 1506
Size: 569 111 726 100
Fragments arranged by size:
726 569 111 100
BsmI GAATG_Cn'
Cuts at: 0 299 1506
Size: 299 1207
Bsp1286I G_dGCh'C
Cuts at: 0 12 569 680 1406 1506
Size: 12 557 111 726 100
Fragments arranged by size:
726 557 111 100 12
BspHI T'CATG_A
Cuts at: 0 777 1506
Size: 777 729
BsrDI GCAATG_nn'
Cuts at: 0 133 1506
Size: 133 1373
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1008 1506
Size: 1008 498
BstBI TT'CG_AA
Cuts at: 0 291 1506
Size: 291 1215
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1105 1506
Size: 1105 401
Bsu36I CC'TnA_GG
Cuts at: 0 989 1506
Size: 989 517
BtgI C'CryG_G
Cuts at: 0 835 1506
Size: 835 671
BtsI GCAGTG_nn'
Cuts at: 0 239 1009 1192 1506
Size: 239 770 183 314
Fragments arranged by size:
770 314 239 183
ClaI AT'CG_AT
Cuts at: 0 482 1506
Size: 482 1024
DraIII CAC_nnn'GTG
Cuts at: 0 827 1506
Size: 827 679
EaeI y'GGCC_r
Cuts at: 0 26 87 689 1506
Size: 26 61 602 817
Fragments arranged by size:
817 602 61 26
EarI CTCTTCn'nnn_
Cuts at: 0 149 343 1506
Size: 149 194 1163
Fragments arranged by size:
1163 194 149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 212 370 438 455 938 1506
Size: 34 178 158 68 17 483 568
Fragments arranged by size:
568 483 178 158 68 34 17
EcoO109I rG'GnC_Cy
Cuts at: 0 531 1506
Size: 531 975
EcoRI G'AATT_C
Cuts at: 0 443 1394 1506
Size: 443 951 112
Fragments arranged by size:
951 443 112
EcoRV GAT'ATC
Cuts at: 0 459 1506
Size: 459 1047
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1143 1175 1240 1272 1506
Size: 1143 32 65 32 234
Fragments arranged by size:
1143 234 65 32 32
HincII GTy'rAC
Cuts at: 0 1169 1506
Size: 1169 337
HpaI GTT'AAC
Cuts at: 0 1169 1506
Size: 1169 337
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 1506
Size: 523 983
MslI CAynn'nnrTG
Cuts at: 0 1247 1506
Size: 1247 259
MspA1I CmG'CkG
Cuts at: 0 695 1309 1506
Size: 695 614 197
Fragments arranged by size:
695 614 197
NcoI C'CATG_G
Cuts at: 0 835 1506
Size: 835 671
NheI G'CTAG_C
Cuts at: 0 70 1506
Size: 70 1436
NsiI A_TGCA'T
Cuts at: 0 1140 1506
Size: 1140 366
NspI r_CATG'y
Cuts at: 0 1448 1506
Size: 1448 58
PflMI CCAn_nnn'nTGG
Cuts at: 0 96 874 1506
Size: 96 778 632
Fragments arranged by size:
778 632 96
PfoI T'CCnGG_A
Cuts at: 0 79 1506
Size: 79 1427
PpuMI rG'GwC_Cy
Cuts at: 0 531 1506
Size: 531 975
PstI C_TGCA'G
Cuts at: 0 1116 1199 1506
Size: 1116 83 307
Fragments arranged by size:
1116 307 83
PvuII CAG'CTG
Cuts at: 0 695 1309 1506
Size: 695 614 197
Fragments arranged by size:
695 614 197
SanDI GG'GwC_CC
Cuts at: 0 531 1506
Size: 531 975
SfcI C'TryA_G
Cuts at: 0 111 1112 1195 1506
Size: 111 1001 83 311
Fragments arranged by size:
1001 311 111 83
StyI C'CwwG_G
Cuts at: 0 487 835 1162 1506
Size: 487 348 327 344
Fragments arranged by size:
487 348 344 327
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 88 1506
Size: 88 1418
TatI w'GTAC_w
Cuts at: 0 389 630 1506
Size: 389 241 876
Fragments arranged by size:
876 389 241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 511 543 588 620 1385 1417 1506
Size: 511 32 45 32 765 32 89
Fragments arranged by size:
765 511 89 45 32 32 32
XbaI T'CTAG_A
Cuts at: 0 1293 1506
Size: 1293 213
XmnI GAAnn'nnTTC
Cuts at: 0 921 1506
Size: 921 585
Enzymes that do cut:
AcuI AlwNI ApaLI ApoI AseI AvaI AvrII BanI
BanII BcgI BfrBI BlpI Bme1580I BmrI BmtI BplI
BpmI BsaAI BsaBI BsaXI BsgI BsiHKAI BsmI Bsp1286I
BspHI BsrDI BstAPI BstBI BstXI Bsu36I BtgI BtsI
ClaI DraIII EaeI EarI Eco57MI EcoO109I EcoRI EcoRV
Hin4I HincII HpaI MmeI MslI MspA1I NcoI NheI
NsiI NspI PflMI PfoI PpuMI PstI PvuII SanDI
SfcI StyI TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI AscI AsiSI
BaeI BamHI BbeI BbsI BbvCI BciVI BclI BglI
BglII BmgBI Bpu10I BpuEI BsaI BsaHI BsaWI BseRI
BseYI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstEII BstYI BstZ17I BtgZI CspCI
DraI DrdI EagI EciI EcoICRI EcoNI FalI FseI
FspI FspAI HaeII HindIII KasI KpnI MfeI MluI
MscI NaeI NarI NdeI NgoMIV NotI NruI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XcmI XhoI XmaI ZraI