Restriction map for
pF1KB8384
(Linear) (Six-base) MAPSORT of: KIBB8384.seq Check: 7212 from: 1 to: 1848
KIBB8384.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AccI GT'mk_AC
Cuts at: 0 1754 1848
Size: 1754 94
Acc65I G'GTAC_C
Cuts at: 0 106 1848
Size: 106 1742
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 181 642 776 1052 1322 1848
Size: 76 105 461 134 276 270 526
Fragments arranged by size:
526 461 276 270 134 105 76
AflII C'TTAA_G
Cuts at: 0 736 1848
Size: 736 1112
AflIII A'CryG_T
Cuts at: 0 266 1848
Size: 266 1582
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 983 1848
Size: 983 865
AlwNI CAG_nnn'CTG
Cuts at: 0 1129 1462 1848
Size: 1129 333 386
Fragments arranged by size:
1129 386 333
ApaI G_GGCC'C
Cuts at: 0 51 568 1008 1636 1848
Size: 51 517 440 628 212
Fragments arranged by size:
628 517 440 212 51
ApoI r'AATT_y
Cuts at: 0 163 1437 1670 1848
Size: 163 1274 233 178
Fragments arranged by size:
1274 233 178 163
AvaI C'yCGr_G
Cuts at: 0 587 776 1001 1226 1848
Size: 587 189 225 225 622
Fragments arranged by size:
622 587 225 225 189
BamHI G'GATC_C
Cuts at: 0 143 284 641 1848
Size: 143 141 357 1207
Fragments arranged by size:
1207 357 143 141
BanI G'GyrC_C
Cuts at: 0 106 1072 1116 1400 1848
Size: 106 966 44 284 448
Fragments arranged by size:
966 448 284 106 44
BanII G_rGCy'C
Cuts at: 0 31 51 568 831 937 1008 1203 1513
Size: 31 20 517 263 106 71 195 310
Cuts at: 1513 1636 1848
Size: 123 212
Fragments arranged by size:
517 310 263 212 195 123 106 71
31 20
BbeI G_GCGC'C
Cuts at: 0 1404 1848
Size: 1404 444
BbsI GAAGACnn'nnnn_
Cuts at: 0 505 1848
Size: 505 1343
BbvCI CC'TCA_GC
Cuts at: 0 65 1848
Size: 65 1783
BciVI GTATCCnnnnn_n'
Cuts at: 0 1362 1848
Size: 1362 486
BglI GCCn_nnn'nGGC
Cuts at: 0 976 1848
Size: 976 872
BglII A'GATC_T
Cuts at: 0 422 1848
Size: 422 1426
Bme1580I G_kGCm'C
Cuts at: 0 51 568 618 1008 1077 1119 1136 1234
Size: 51 517 50 390 69 42 17 98
Cuts at: 1234 1636 1848
Size: 402 212
Fragments arranged by size:
517 402 390 212 98 69 51 50
42 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 47 639 893 1027 1473 1848
Size: 47 592 254 134 446 375
Fragments arranged by size:
592 446 375 254 134 47
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 1556 1848
Size: 242 1314 292
Fragments arranged by size:
1314 292 242
Bpu10I CC'TnA_GC
Cuts at: 0 65 1512 1848
Size: 65 1447 336
Fragments arranged by size:
1447 336 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1435 1470 1848
Size: 1435 35 378
Fragments arranged by size:
1435 378 35
BsaAI yAC'GTr
Cuts at: 0 904 1848
Size: 904 944
BsaBI GATnn'nnATC
Cuts at: 0 347 1848
Size: 347 1501
BsaHI Gr'CG_yC
Cuts at: 0 1401 1848
Size: 1401 447
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 253 283 595 625 1848
Size: 253 30 312 30 1223
Fragments arranged by size:
1223 312 253 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 274 1848
Size: 274 1574
BseYI C'CCAG_C
Cuts at: 0 70 579 854 1122 1643 1848
Size: 70 509 275 268 521 205
Fragments arranged by size:
521 509 275 268 205 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 704 1745 1848
Size: 145 559 1041 103
Fragments arranged by size:
1041 559 145 103
BsiHKAI G_wGCw'C
Cuts at: 0 308 937 1203 1848
Size: 308 629 266 645
Fragments arranged by size:
645 629 308 266
Bsp1286I G_dGCh'C
Cuts at: 0 31 51 308 568 618 831 937 1008
Size: 31 20 257 260 50 213 106 71
Cuts at: 1008 1077 1119 1136 1203 1234 1513 1636 1848
Size: 69 42 17 67 31 279 123 212
Fragments arranged by size:
279 260 257 213 212 123 106 71
69 67 50 42 31 31 20 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 951 965 1848
Size: 951 14 883
Fragments arranged by size:
951 883 14
BsrBI CCG'CTC
Cuts at: 0 639 912 1848
Size: 639 273 936
Fragments arranged by size:
936 639 273
BsrDI GCAATG_nn'
Cuts at: 0 261 1848
Size: 261 1587
BsrFI r'CCGG_y
Cuts at: 0 889 1848
Size: 889 959
BsrGI T'GTAC_A
Cuts at: 0 156 1848
Size: 156 1692
BstAPI GCAn_nnn'nTGC
Cuts at: 0 613 1381 1588 1848
Size: 613 768 207 260
Fragments arranged by size:
768 613 260 207
BstEII G'GTnAC_C
Cuts at: 0 1261 1490 1848
Size: 1261 229 358
Fragments arranged by size:
1261 358 229
BstYI r'GATC_y
Cuts at: 0 143 284 422 641 867 1848
Size: 143 141 138 219 226 981
Fragments arranged by size:
981 226 219 143 141 138
Bsu36I CC'TnA_GG
Cuts at: 0 207 863 1848
Size: 207 656 985
Fragments arranged by size:
985 656 207
BtgI C'CryG_G
Cuts at: 0 743 1466 1495 1848
Size: 743 723 29 353
Fragments arranged by size:
743 723 353 29
BtsI GCAGTG_nn'
Cuts at: 0 1090 1757 1848
Size: 1090 667 91
Fragments arranged by size:
1090 667 91
DrdI GACnn_nn'nnGTC
Cuts at: 0 507 1285 1848
Size: 507 778 563
Fragments arranged by size:
778 563 507
EaeI y'GGCC_r
Cuts at: 0 456 1848
Size: 456 1392
EarI CTCTTCn'nnn_
Cuts at: 0 296 752 913 1848
Size: 296 456 161 935
Fragments arranged by size:
935 456 296 161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1013 1848
Size: 1013 835
EcoICRI GAG'CTC
Cuts at: 0 935 1201 1848
Size: 935 266 647
Fragments arranged by size:
935 647 266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 181 242 642 776 1052 1322 1556
Size: 76 105 61 400 134 276 270 234
Cuts at: 1556 1848
Size: 292
Fragments arranged by size:
400 292 276 270 234 134 105 76
61
EcoO109I rG'GnC_Cy
Cuts at: 0 47 48 211 1005 1632 1633 1848
Size: 47 1 163 794 627 1 215
Fragments arranged by size:
794 627 215 163 47 1 1
EcoRI G'AATT_C
Cuts at: 0 1437 1848
Size: 1437 411
HaeII r_GCGC'y
Cuts at: 0 1404 1848
Size: 1404 444
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 217 249 1848
Size: 217 32 1599
Fragments arranged by size:
1599 217 32
KasI G'GCGC_C
Cuts at: 0 1400 1848
Size: 1400 448
KpnI G_GTAC'C
Cuts at: 0 110 1848
Size: 110 1738
MslI CAynn'nnrTG
Cuts at: 0 728 1144 1848
Size: 728 416 704
Fragments arranged by size:
728 704 416
MspA1I CmG'CkG
Cuts at: 0 1129 1300 1462 1534 1848
Size: 1129 171 162 72 314
Fragments arranged by size:
1129 314 171 162 72
NarI GG'CG_CC
Cuts at: 0 1401 1848
Size: 1401 447
NcoI C'CATG_G
Cuts at: 0 743 1466 1495 1848
Size: 743 723 29 353
Fragments arranged by size:
743 723 353 29
NspI r_CATG'y
Cuts at: 0 270 727 772 1795 1848
Size: 270 457 45 1023 53
Fragments arranged by size:
1023 457 270 53 45
PasI CC'CwG_GG
Cuts at: 0 1593 1848
Size: 1593 255
PciI A'CATG_T
Cuts at: 0 266 1848
Size: 266 1582
PflMI CCAn_nnn'nTGG
Cuts at: 0 802 823 1848
Size: 802 21 1025
Fragments arranged by size:
1025 802 21
PmlI CAC'GTG
Cuts at: 0 904 1848
Size: 904 944
PpuMI rG'GwC_Cy
Cuts at: 0 211 1848
Size: 211 1637
PshAI GACnn'nnGTC
Cuts at: 0 317 1848
Size: 317 1531
PspOMI G'GGCC_C
Cuts at: 0 47 564 1004 1632 1848
Size: 47 517 440 628 216
Fragments arranged by size:
628 517 440 216 47
PstI C_TGCA'G
Cuts at: 0 962 1848
Size: 962 886
PvuII CAG'CTG
Cuts at: 0 1129 1300 1462 1534 1848
Size: 1129 171 162 72 314
Fragments arranged by size:
1129 314 171 162 72
SacI G_AGCT'C
Cuts at: 0 937 1203 1848
Size: 937 266 645
Fragments arranged by size:
937 645 266
SapI GCTCTTCn'nnn_
Cuts at: 0 296 1848
Size: 296 1552
SbfI CC_TGCA'GG
Cuts at: 0 962 1848
Size: 962 886
ScaI AGT'ACT
Cuts at: 0 358 1586 1848
Size: 358 1228 262
Fragments arranged by size:
1228 358 262
SfcI C'TryA_G
Cuts at: 0 276 480 691 958 1848
Size: 276 204 211 267 890
Fragments arranged by size:
890 276 267 211 204
SfoI GGC'GCC
Cuts at: 0 1402 1848
Size: 1402 446
SmaI CCC'GGG
Cuts at: 0 1003 1228 1848
Size: 1003 225 620
Fragments arranged by size:
1003 620 225
SmlI C'TyrA_G
Cuts at: 0 587 736 1450 1485 1848
Size: 587 149 714 35 363
Fragments arranged by size:
714 587 363 149 35
SspI AAT'ATT
Cuts at: 0 475 1848
Size: 475 1373
StyI C'CwwG_G
Cuts at: 0 116 503 743 1266 1466 1495 1676 1848
Size: 116 387 240 523 200 29 181 172
Fragments arranged by size:
523 387 240 200 181 172 116 29
TatI w'GTAC_w
Cuts at: 0 156 244 263 356 1584 1848
Size: 156 88 19 93 1228 264
Fragments arranged by size:
1228 264 156 93 88 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 345 377 595 627 906 938 1848
Size: 345 32 218 32 279 32 910
Fragments arranged by size:
910 345 279 218 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1502 1848
Size: 1502 346
XhoI C'TCGA_G
Cuts at: 0 587 1848
Size: 587 1261
XmaI C'CCGG_G
Cuts at: 0 1001 1226 1848
Size: 1001 225 622
Fragments arranged by size:
1001 622 225
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AlfI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII
BstYI Bsu36I BtgI BtsI DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I KasI KpnI
MslI MspA1I NarI NcoI NspI PasI PciI PflMI
PmlI PpuMI PshAI PspOMI PstI PvuII SacI SapI
SbfI ScaI SfcI SfoI SmaI SmlI SspI StyI
TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AhdI AleI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BcgI BclI
BfrBI BlpI BmgBI BmtI BplI BsaI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BssHII BssSI BstBI
BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII EagI
EcoNI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI MfeI MluI MmeI MscI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PfoI PmeI PpiI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
StuI SwaI TaqII Tth111I XbaI XmnI ZraI