(Linear) (Six-base) MAPSORT of: KIBB8385.seq Check: 1587 from: 1 to: 1269 KIBB8385.orf With 182 enzymes: SgfI * March 3, 2008 11:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 313 903 1269 Size: 313 590 366 Fragments arranged by size: 590 366 313 AccI GT'mk_AC Cuts at: 0 941 1269 Size: 941 328 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 666 1269 Size: 666 603 AflIII A'CryG_T Cuts at: 0 936 1250 1269 Size: 936 314 19 Fragments arranged by size: 936 314 19 AhdI GACnn_n'nnGTC Cuts at: 0 744 1269 Size: 744 525 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1126 1269 Size: 1126 143 AlwNI CAG_nnn'CTG Cuts at: 0 380 1269 Size: 380 889 ApaI G_GGCC'C Cuts at: 0 220 702 1269 Size: 220 482 567 Fragments arranged by size: 567 482 220 AscI GG'CGCG_CC Cuts at: 0 140 1269 Size: 140 1129 AvaI C'yCGr_G Cuts at: 0 135 162 1269 Size: 135 27 1107 Fragments arranged by size: 1107 135 27 BanI G'GyrC_C Cuts at: 0 106 819 1269 Size: 106 713 450 Fragments arranged by size: 713 450 106 BanII G_rGCy'C Cuts at: 0 136 220 590 702 1269 Size: 136 84 370 112 567 Fragments arranged by size: 567 370 136 112 84 BbeI G_GCGC'C Cuts at: 0 110 823 1269 Size: 110 713 446 Fragments arranged by size: 713 446 110 BglI GCCn_nnn'nGGC Cuts at: 0 1100 1269 Size: 1100 169 Bme1580I G_kGCm'C Cuts at: 0 220 702 1269 Size: 220 482 567 Fragments arranged by size: 567 482 220 BmgBI CAC'GTC Cuts at: 0 42 599 939 986 1269 Size: 42 557 340 47 283 Fragments arranged by size: 557 340 283 47 42 BmrI ACTGGGnnnn_n' Cuts at: 0 789 1269 Size: 789 480 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 786 1075 1269 Size: 786 289 194 Fragments arranged by size: 786 289 194 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 412 910 1269 Size: 412 498 359 Fragments arranged by size: 498 412 359 BsaAI yAC'GTr Cuts at: 0 816 1065 1269 Size: 816 249 204 Fragments arranged by size: 816 249 204 BsaHI Gr'CG_yC Cuts at: 0 107 820 992 1269 Size: 107 713 172 277 Fragments arranged by size: 713 277 172 107 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 348 1072 1083 1134 1269 Size: 348 724 11 51 135 Fragments arranged by size: 724 348 135 51 11 BseYI C'CCAG_C Cuts at: 0 491 1090 1269 Size: 491 599 179 Fragments arranged by size: 599 491 179 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 796 1269 Size: 796 473 BsiEI CG_ry'CG Cuts at: 0 870 1269 Size: 870 399 BsiHKAI G_wGCw'C Cuts at: 0 785 802 1267 1269 Size: 785 17 465 2 Fragments arranged by size: 785 465 17 2 BsmI GAATG_Cn' Cuts at: 0 485 1269 Size: 485 784 Bsp1286I G_dGCh'C Cuts at: 0 136 220 590 702 785 802 1267 1269 Size: 136 84 370 112 83 17 465 2 Fragments arranged by size: 465 370 136 112 84 83 17 2 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 313 903 1269 Size: 313 590 366 Fragments arranged by size: 590 366 313 BsrBI CCG'CTC Cuts at: 0 281 1269 Size: 281 988 BsrDI GCAATG_nn' Cuts at: 0 11 1269 Size: 11 1258 BsrFI r'CCGG_y Cuts at: 0 109 838 1269 Size: 109 729 431 Fragments arranged by size: 729 431 109 BssHII G'CGCG_C Cuts at: 0 140 579 670 1269 Size: 140 439 91 599 Fragments arranged by size: 599 439 140 91 BstAPI GCAn_nnn'nTGC Cuts at: 0 410 617 1269 Size: 410 207 652 Fragments arranged by size: 652 410 207 BstYI r'GATC_y Cuts at: 0 27 723 1269 Size: 27 696 546 Fragments arranged by size: 696 546 27 BtgI C'CryG_G Cuts at: 0 90 1269 Size: 90 1179 BtsI GCAGTG_nn' Cuts at: 0 543 1051 1269 Size: 543 508 218 Fragments arranged by size: 543 508 218 EaeI y'GGCC_r Cuts at: 0 44 294 540 665 830 1269 Size: 44 250 246 125 165 439 Fragments arranged by size: 439 250 246 165 125 44 EarI CTCTTCn'nnn_ Cuts at: 0 685 1168 1269 Size: 685 483 101 Fragments arranged by size: 685 483 101 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 666 786 1075 1269 Size: 666 120 289 194 Fragments arranged by size: 666 289 194 120 EcoO109I rG'GnC_Cy Cuts at: 0 216 528 1269 Size: 216 312 741 Fragments arranged by size: 741 312 216 FspI TGC'GCA Cuts at: 0 1050 1132 1269 Size: 1050 82 137 Fragments arranged by size: 1050 137 82 FspAI rTGC'GCAy Cuts at: 0 1050 1269 Size: 1050 219 HaeII r_GCGC'y Cuts at: 0 110 181 689 823 934 1269 Size: 110 71 508 134 111 335 Fragments arranged by size: 508 335 134 111 110 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 730 762 1269 Size: 730 32 507 Fragments arranged by size: 730 507 32 HincII GTy'rAC Cuts at: 0 942 1269 Size: 942 327 KasI G'GCGC_C Cuts at: 0 106 819 1269 Size: 106 713 450 Fragments arranged by size: 713 450 106 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 511 986 1269 Size: 511 475 283 Fragments arranged by size: 511 475 283 MspA1I CmG'CkG Cuts at: 0 20 92 810 959 1269 Size: 20 72 718 149 310 Fragments arranged by size: 718 310 149 72 20 NaeI GCC'GGC Cuts at: 0 111 840 1269 Size: 111 729 429 Fragments arranged by size: 729 429 111 NarI GG'CG_CC Cuts at: 0 107 820 1269 Size: 107 713 449 Fragments arranged by size: 713 449 107 NgoMIV G'CCGG_C Cuts at: 0 109 838 1269 Size: 109 729 431 Fragments arranged by size: 729 431 109 NspI r_CATG'y Cuts at: 0 365 624 850 1254 1269 Size: 365 259 226 404 15 Fragments arranged by size: 404 365 259 226 15 PciI A'CATG_T Cuts at: 0 1250 1269 Size: 1250 19 PfoI T'CCnGG_A Cuts at: 0 719 905 1269 Size: 719 186 364 Fragments arranged by size: 719 364 186 PmlI CAC'GTG Cuts at: 0 816 1269 Size: 816 453 PpuMI rG'GwC_Cy Cuts at: 0 528 1269 Size: 528 741 PspOMI G'GGCC_C Cuts at: 0 216 698 1269 Size: 216 482 571 Fragments arranged by size: 571 482 216 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 340 372 1269 Size: 340 32 897 Fragments arranged by size: 897 340 32 PstI C_TGCA'G Cuts at: 0 731 1034 1269 Size: 731 303 235 Fragments arranged by size: 731 303 235 PvuII CAG'CTG Cuts at: 0 20 959 1269 Size: 20 939 310 Fragments arranged by size: 939 310 20 SacII CC_GC'GG Cuts at: 0 93 1269 Size: 93 1176 SalI G'TCGA_C Cuts at: 0 940 1269 Size: 940 329 SanDI GG'GwC_CC Cuts at: 0 528 1269 Size: 528 741 SapI GCTCTTCn'nnn_ Cuts at: 0 685 1168 1269 Size: 685 483 101 Fragments arranged by size: 685 483 101 SbfI CC_TGCA'GG Cuts at: 0 1034 1269 Size: 1034 235 SexAI A'CCwGG_T Cuts at: 0 740 1269 Size: 740 529 SfcI C'TryA_G Cuts at: 0 727 1030 1269 Size: 727 303 239 Fragments arranged by size: 727 303 239 SfoI GGC'GCC Cuts at: 0 108 821 1269 Size: 108 713 448 Fragments arranged by size: 713 448 108 SgrAI Cr'CCGG_yG Cuts at: 0 109 1269 Size: 109 1160 SmaI CCC'GGG Cuts at: 0 164 1269 Size: 164 1105 SmlI C'TyrA_G Cuts at: 0 427 925 1269 Size: 427 498 344 Fragments arranged by size: 498 427 344 SphI G_CATG'C Cuts at: 0 624 850 1269 Size: 624 226 419 Fragments arranged by size: 624 419 226 TatI w'GTAC_w Cuts at: 0 953 1269 Size: 953 316 Tth111I GACn'n_nGTC Cuts at: 0 871 1269 Size: 871 398 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 636 1269 Size: 636 633 XmaI C'CCGG_G Cuts at: 0 162 1269 Size: 162 1107 XmnI GAAnn'nnTTC Cuts at: 0 464 1269 Size: 464 805 Enzymes that do cut: AarI AccI AcuI AflIII AhdI AlfI AlwNI ApaI AscI AvaI BanI BanII BbeI BglI Bme1580I BmgBI BmrI BpmI BpuEI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstYI BtgI BtsI EaeI EarI Eco57MI EcoO109I FspI FspAI HaeII Hin4I HincII KasI MmeI MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmlI PpuMI PspOMI PsrI PstI PvuII SacII SalI SanDI SapI SbfI SexAI SfcI SfoI SgrAI SmaI SmlI SphI TatI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AleI AloI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmtI BplI Bpu10I BsaI BsaBI BsaWI BsaXI BsiWI BsmBI BspEI BspHI BsrGI BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI HindIII HpaI KpnI MfeI MluI MscI MslI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmeI PpiI PshAI PsiI PspXI PvuI RsrII SacI ScaI SfiI SgfI SnaBI SpeI SrfI SspI StuI StyI SwaI TaqII TstI XbaI XhoI ZraI