Restriction map for pF1KB8385
 (Linear) (Six-base) MAPSORT of: KIBB8385.seq  Check: 1587  from: 1  to: 1269



KIBB8385.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     313     903    1269 
   Size:        313     590     366

  Fragments arranged by size: 

                 590     366     313
 
AccI GT'mk_AC

Cuts at:      0     941    1269 
   Size:        941     328
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     666    1269 
   Size:        666     603
 
AflIII A'CryG_T

Cuts at:      0     936    1250    1269 
   Size:        936     314      19

  Fragments arranged by size: 

                 936     314      19
 
AhdI GACnn_n'nnGTC

Cuts at:      0     744    1269 
   Size:        744     525
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1126    1269 
   Size:       1126     143
 
AlwNI CAG_nnn'CTG

Cuts at:      0     380    1269 
   Size:        380     889
 
ApaI G_GGCC'C

Cuts at:      0     220     702    1269 
   Size:        220     482     567

  Fragments arranged by size: 

                 567     482     220
 
AscI GG'CGCG_CC

Cuts at:      0     140    1269 
   Size:        140    1129
 
AvaI C'yCGr_G

Cuts at:      0     135     162    1269 
   Size:        135      27    1107

  Fragments arranged by size: 

                1107     135      27
 
BanI G'GyrC_C

Cuts at:      0     106     819    1269 
   Size:        106     713     450

  Fragments arranged by size: 

                 713     450     106
 
BanII G_rGCy'C

Cuts at:      0     136     220     590     702    1269 
   Size:        136      84     370     112     567

  Fragments arranged by size: 

                 567     370     136     112      84
 
BbeI G_GCGC'C

Cuts at:      0     110     823    1269 
   Size:        110     713     446

  Fragments arranged by size: 

                 713     446     110
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1100    1269 
   Size:       1100     169
 
Bme1580I G_kGCm'C

Cuts at:      0     220     702    1269 
   Size:        220     482     567

  Fragments arranged by size: 

                 567     482     220
 
BmgBI CAC'GTC

Cuts at:      0      42     599     939     986    1269 
   Size:         42     557     340      47     283

  Fragments arranged by size: 

                 557     340     283      47      42
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     789    1269 
   Size:        789     480
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     786    1075    1269 
   Size:        786     289     194

  Fragments arranged by size: 

                 786     289     194
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412     910    1269 
   Size:        412     498     359

  Fragments arranged by size: 

                 498     412     359
 
BsaAI yAC'GTr

Cuts at:      0     816    1065    1269 
   Size:        816     249     204

  Fragments arranged by size: 

                 816     249     204
 
BsaHI Gr'CG_yC

Cuts at:      0     107     820     992    1269 
   Size:        107     713     172     277

  Fragments arranged by size: 

                 713     277     172     107
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     348    1072    1083    1134    1269 
   Size:        348     724      11      51     135

  Fragments arranged by size: 

                 724     348     135      51      11
 
BseYI C'CCAG_C

Cuts at:      0     491    1090    1269 
   Size:        491     599     179

  Fragments arranged by size: 

                 599     491     179
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     796    1269 
   Size:        796     473
 
BsiEI CG_ry'CG

Cuts at:      0     870    1269 
   Size:        870     399
 
BsiHKAI G_wGCw'C

Cuts at:      0     785     802    1267    1269 
   Size:        785      17     465       2

  Fragments arranged by size: 

                 785     465      17       2
 
BsmI GAATG_Cn'

Cuts at:      0     485    1269 
   Size:        485     784
 
Bsp1286I G_dGCh'C

Cuts at:      0     136     220     590     702     785     802    1267    1269 
   Size:        136      84     370     112      83      17     465       2

  Fragments arranged by size: 

                 465     370     136     112      84      83      17       2
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     313     903    1269 
   Size:        313     590     366

  Fragments arranged by size: 

                 590     366     313
 
BsrBI CCG'CTC

Cuts at:      0     281    1269 
   Size:        281     988
 
BsrDI GCAATG_nn'

Cuts at:      0      11    1269 
   Size:         11    1258
 
BsrFI r'CCGG_y

Cuts at:      0     109     838    1269 
   Size:        109     729     431

  Fragments arranged by size: 

                 729     431     109
 
BssHII G'CGCG_C

Cuts at:      0     140     579     670    1269 
   Size:        140     439      91     599

  Fragments arranged by size: 

                 599     439     140      91
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     410     617    1269 
   Size:        410     207     652

  Fragments arranged by size: 

                 652     410     207
 
BstYI r'GATC_y

Cuts at:      0      27     723    1269 
   Size:         27     696     546

  Fragments arranged by size: 

                 696     546      27
 
BtgI C'CryG_G

Cuts at:      0      90    1269 
   Size:         90    1179
 
BtsI GCAGTG_nn'

Cuts at:      0     543    1051    1269 
   Size:        543     508     218

  Fragments arranged by size: 

                 543     508     218
 
EaeI y'GGCC_r

Cuts at:      0      44     294     540     665     830    1269 
   Size:         44     250     246     125     165     439

  Fragments arranged by size: 

                 439     250     246     165     125      44
 
EarI CTCTTCn'nnn_

Cuts at:      0     685    1168    1269 
   Size:        685     483     101

  Fragments arranged by size: 

                 685     483     101
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     666     786    1075    1269 
   Size:        666     120     289     194

  Fragments arranged by size: 

                 666     289     194     120
 
EcoO109I rG'GnC_Cy

Cuts at:      0     216     528    1269 
   Size:        216     312     741

  Fragments arranged by size: 

                 741     312     216
 
FspI TGC'GCA

Cuts at:      0    1050    1132    1269 
   Size:       1050      82     137

  Fragments arranged by size: 

                1050     137      82
 
FspAI rTGC'GCAy

Cuts at:      0    1050    1269 
   Size:       1050     219
 
HaeII r_GCGC'y

Cuts at:      0     110     181     689     823     934    1269 
   Size:        110      71     508     134     111     335

  Fragments arranged by size: 

                 508     335     134     111     110      71
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     730     762    1269 
   Size:        730      32     507

  Fragments arranged by size: 

                 730     507      32
 
HincII GTy'rAC

Cuts at:      0     942    1269 
   Size:        942     327
 
KasI G'GCGC_C

Cuts at:      0     106     819    1269 
   Size:        106     713     450

  Fragments arranged by size: 

                 713     450     106
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     511     986    1269 
   Size:        511     475     283

  Fragments arranged by size: 

                 511     475     283
 
MspA1I CmG'CkG

Cuts at:      0      20      92     810     959    1269 
   Size:         20      72     718     149     310

  Fragments arranged by size: 

                 718     310     149      72      20
 
NaeI GCC'GGC

Cuts at:      0     111     840    1269 
   Size:        111     729     429

  Fragments arranged by size: 

                 729     429     111
 
NarI GG'CG_CC

Cuts at:      0     107     820    1269 
   Size:        107     713     449

  Fragments arranged by size: 

                 713     449     107
 
NgoMIV G'CCGG_C

Cuts at:      0     109     838    1269 
   Size:        109     729     431

  Fragments arranged by size: 

                 729     431     109
 
NspI r_CATG'y

Cuts at:      0     365     624     850    1254    1269 
   Size:        365     259     226     404      15

  Fragments arranged by size: 

                 404     365     259     226      15
 
PciI A'CATG_T

Cuts at:      0    1250    1269 
   Size:       1250      19
 
PfoI T'CCnGG_A

Cuts at:      0     719     905    1269 
   Size:        719     186     364

  Fragments arranged by size: 

                 719     364     186
 
PmlI CAC'GTG

Cuts at:      0     816    1269 
   Size:        816     453
 
PpuMI rG'GwC_Cy

Cuts at:      0     528    1269 
   Size:        528     741
 
PspOMI G'GGCC_C

Cuts at:      0     216     698    1269 
   Size:        216     482     571

  Fragments arranged by size: 

                 571     482     216
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     340     372    1269 
   Size:        340      32     897

  Fragments arranged by size: 

                 897     340      32
 
PstI C_TGCA'G

Cuts at:      0     731    1034    1269 
   Size:        731     303     235

  Fragments arranged by size: 

                 731     303     235
 
PvuII CAG'CTG

Cuts at:      0      20     959    1269 
   Size:         20     939     310

  Fragments arranged by size: 

                 939     310      20
 
SacII CC_GC'GG

Cuts at:      0      93    1269 
   Size:         93    1176
 
SalI G'TCGA_C

Cuts at:      0     940    1269 
   Size:        940     329
 
SanDI GG'GwC_CC

Cuts at:      0     528    1269 
   Size:        528     741
 
SapI GCTCTTCn'nnn_

Cuts at:      0     685    1168    1269 
   Size:        685     483     101

  Fragments arranged by size: 

                 685     483     101
 
SbfI CC_TGCA'GG

Cuts at:      0    1034    1269 
   Size:       1034     235
 
SexAI A'CCwGG_T

Cuts at:      0     740    1269 
   Size:        740     529
 
SfcI C'TryA_G

Cuts at:      0     727    1030    1269 
   Size:        727     303     239

  Fragments arranged by size: 

                 727     303     239
 
SfoI GGC'GCC

Cuts at:      0     108     821    1269 
   Size:        108     713     448

  Fragments arranged by size: 

                 713     448     108
 
SgrAI Cr'CCGG_yG

Cuts at:      0     109    1269 
   Size:        109    1160
 
SmaI CCC'GGG

Cuts at:      0     164    1269 
   Size:        164    1105
 
SmlI C'TyrA_G

Cuts at:      0     427     925    1269 
   Size:        427     498     344

  Fragments arranged by size: 

                 498     427     344
 
SphI G_CATG'C

Cuts at:      0     624     850    1269 
   Size:        624     226     419

  Fragments arranged by size: 

                 624     419     226
 
TatI w'GTAC_w

Cuts at:      0     953    1269 
   Size:        953     316
 
Tth111I GACn'n_nGTC

Cuts at:      0     871    1269 
   Size:        871     398
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     636    1269 
   Size:        636     633
 
XmaI C'CCGG_G

Cuts at:      0     162    1269 
   Size:        162    1107
 
XmnI GAAnn'nnTTC

Cuts at:      0     464    1269 
   Size:        464     805
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AhdI     AlfI    AlwNI     ApaI
     AscI     AvaI     BanI    BanII     BbeI     BglI Bme1580I    BmgBI
     BmrI     BpmI    BpuEI    BsaAI    BsaHI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
   BssHII   BstAPI    BstYI     BtgI     BtsI     EaeI     EarI  Eco57MI
 EcoO109I     FspI    FspAI    HaeII    Hin4I   HincII     KasI     MmeI
   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI     PfoI     PmlI
    PpuMI   PspOMI     PsrI     PstI    PvuII    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfcI     SfoI    SgrAI     SmaI     SmlI
     SphI     TatI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AleI     AloI
    ApaLI     ApoI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI     BmtI
     BplI   Bpu10I     BsaI    BsaBI    BsaWI    BsaXI    BsiWI    BsmBI
    BspEI    BspHI    BsrGI    BssSI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI  HindIII
     HpaI     KpnI     MfeI     MluI     MscI     MslI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PmeI
     PpiI    PshAI     PsiI    PspXI     PvuI    RsrII     SacI     ScaI
     SfiI     SgfI    SnaBI     SpeI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TstI     XbaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com