Restriction map for
pF1KB8385
(Linear) (Six-base) MAPSORT of: KIBB8385.seq Check: 1587 from: 1 to: 1269
KIBB8385.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 313 903 1269
Size: 313 590 366
Fragments arranged by size:
590 366 313
AccI GT'mk_AC
Cuts at: 0 941 1269
Size: 941 328
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 666 1269
Size: 666 603
AflIII A'CryG_T
Cuts at: 0 936 1250 1269
Size: 936 314 19
Fragments arranged by size:
936 314 19
AhdI GACnn_n'nnGTC
Cuts at: 0 744 1269
Size: 744 525
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1126 1269
Size: 1126 143
AlwNI CAG_nnn'CTG
Cuts at: 0 380 1269
Size: 380 889
ApaI G_GGCC'C
Cuts at: 0 220 702 1269
Size: 220 482 567
Fragments arranged by size:
567 482 220
AscI GG'CGCG_CC
Cuts at: 0 140 1269
Size: 140 1129
AvaI C'yCGr_G
Cuts at: 0 135 162 1269
Size: 135 27 1107
Fragments arranged by size:
1107 135 27
BanI G'GyrC_C
Cuts at: 0 106 819 1269
Size: 106 713 450
Fragments arranged by size:
713 450 106
BanII G_rGCy'C
Cuts at: 0 136 220 590 702 1269
Size: 136 84 370 112 567
Fragments arranged by size:
567 370 136 112 84
BbeI G_GCGC'C
Cuts at: 0 110 823 1269
Size: 110 713 446
Fragments arranged by size:
713 446 110
BglI GCCn_nnn'nGGC
Cuts at: 0 1100 1269
Size: 1100 169
Bme1580I G_kGCm'C
Cuts at: 0 220 702 1269
Size: 220 482 567
Fragments arranged by size:
567 482 220
BmgBI CAC'GTC
Cuts at: 0 42 599 939 986 1269
Size: 42 557 340 47 283
Fragments arranged by size:
557 340 283 47 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 789 1269
Size: 789 480
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 786 1075 1269
Size: 786 289 194
Fragments arranged by size:
786 289 194
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 910 1269
Size: 412 498 359
Fragments arranged by size:
498 412 359
BsaAI yAC'GTr
Cuts at: 0 816 1065 1269
Size: 816 249 204
Fragments arranged by size:
816 249 204
BsaHI Gr'CG_yC
Cuts at: 0 107 820 992 1269
Size: 107 713 172 277
Fragments arranged by size:
713 277 172 107
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 348 1072 1083 1134 1269
Size: 348 724 11 51 135
Fragments arranged by size:
724 348 135 51 11
BseYI C'CCAG_C
Cuts at: 0 491 1090 1269
Size: 491 599 179
Fragments arranged by size:
599 491 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 1269
Size: 796 473
BsiEI CG_ry'CG
Cuts at: 0 870 1269
Size: 870 399
BsiHKAI G_wGCw'C
Cuts at: 0 785 802 1267 1269
Size: 785 17 465 2
Fragments arranged by size:
785 465 17 2
BsmI GAATG_Cn'
Cuts at: 0 485 1269
Size: 485 784
Bsp1286I G_dGCh'C
Cuts at: 0 136 220 590 702 785 802 1267 1269
Size: 136 84 370 112 83 17 465 2
Fragments arranged by size:
465 370 136 112 84 83 17 2
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 313 903 1269
Size: 313 590 366
Fragments arranged by size:
590 366 313
BsrBI CCG'CTC
Cuts at: 0 281 1269
Size: 281 988
BsrDI GCAATG_nn'
Cuts at: 0 11 1269
Size: 11 1258
BsrFI r'CCGG_y
Cuts at: 0 109 838 1269
Size: 109 729 431
Fragments arranged by size:
729 431 109
BssHII G'CGCG_C
Cuts at: 0 140 579 670 1269
Size: 140 439 91 599
Fragments arranged by size:
599 439 140 91
BstAPI GCAn_nnn'nTGC
Cuts at: 0 410 617 1269
Size: 410 207 652
Fragments arranged by size:
652 410 207
BstYI r'GATC_y
Cuts at: 0 27 723 1269
Size: 27 696 546
Fragments arranged by size:
696 546 27
BtgI C'CryG_G
Cuts at: 0 90 1269
Size: 90 1179
BtsI GCAGTG_nn'
Cuts at: 0 543 1051 1269
Size: 543 508 218
Fragments arranged by size:
543 508 218
EaeI y'GGCC_r
Cuts at: 0 44 294 540 665 830 1269
Size: 44 250 246 125 165 439
Fragments arranged by size:
439 250 246 165 125 44
EarI CTCTTCn'nnn_
Cuts at: 0 685 1168 1269
Size: 685 483 101
Fragments arranged by size:
685 483 101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 666 786 1075 1269
Size: 666 120 289 194
Fragments arranged by size:
666 289 194 120
EcoO109I rG'GnC_Cy
Cuts at: 0 216 528 1269
Size: 216 312 741
Fragments arranged by size:
741 312 216
FspI TGC'GCA
Cuts at: 0 1050 1132 1269
Size: 1050 82 137
Fragments arranged by size:
1050 137 82
FspAI rTGC'GCAy
Cuts at: 0 1050 1269
Size: 1050 219
HaeII r_GCGC'y
Cuts at: 0 110 181 689 823 934 1269
Size: 110 71 508 134 111 335
Fragments arranged by size:
508 335 134 111 110 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 730 762 1269
Size: 730 32 507
Fragments arranged by size:
730 507 32
HincII GTy'rAC
Cuts at: 0 942 1269
Size: 942 327
KasI G'GCGC_C
Cuts at: 0 106 819 1269
Size: 106 713 450
Fragments arranged by size:
713 450 106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 986 1269
Size: 511 475 283
Fragments arranged by size:
511 475 283
MspA1I CmG'CkG
Cuts at: 0 20 92 810 959 1269
Size: 20 72 718 149 310
Fragments arranged by size:
718 310 149 72 20
NaeI GCC'GGC
Cuts at: 0 111 840 1269
Size: 111 729 429
Fragments arranged by size:
729 429 111
NarI GG'CG_CC
Cuts at: 0 107 820 1269
Size: 107 713 449
Fragments arranged by size:
713 449 107
NgoMIV G'CCGG_C
Cuts at: 0 109 838 1269
Size: 109 729 431
Fragments arranged by size:
729 431 109
NspI r_CATG'y
Cuts at: 0 365 624 850 1254 1269
Size: 365 259 226 404 15
Fragments arranged by size:
404 365 259 226 15
PciI A'CATG_T
Cuts at: 0 1250 1269
Size: 1250 19
PfoI T'CCnGG_A
Cuts at: 0 719 905 1269
Size: 719 186 364
Fragments arranged by size:
719 364 186
PmlI CAC'GTG
Cuts at: 0 816 1269
Size: 816 453
PpuMI rG'GwC_Cy
Cuts at: 0 528 1269
Size: 528 741
PspOMI G'GGCC_C
Cuts at: 0 216 698 1269
Size: 216 482 571
Fragments arranged by size:
571 482 216
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 340 372 1269
Size: 340 32 897
Fragments arranged by size:
897 340 32
PstI C_TGCA'G
Cuts at: 0 731 1034 1269
Size: 731 303 235
Fragments arranged by size:
731 303 235
PvuII CAG'CTG
Cuts at: 0 20 959 1269
Size: 20 939 310
Fragments arranged by size:
939 310 20
SacII CC_GC'GG
Cuts at: 0 93 1269
Size: 93 1176
SalI G'TCGA_C
Cuts at: 0 940 1269
Size: 940 329
SanDI GG'GwC_CC
Cuts at: 0 528 1269
Size: 528 741
SapI GCTCTTCn'nnn_
Cuts at: 0 685 1168 1269
Size: 685 483 101
Fragments arranged by size:
685 483 101
SbfI CC_TGCA'GG
Cuts at: 0 1034 1269
Size: 1034 235
SexAI A'CCwGG_T
Cuts at: 0 740 1269
Size: 740 529
SfcI C'TryA_G
Cuts at: 0 727 1030 1269
Size: 727 303 239
Fragments arranged by size:
727 303 239
SfoI GGC'GCC
Cuts at: 0 108 821 1269
Size: 108 713 448
Fragments arranged by size:
713 448 108
SgrAI Cr'CCGG_yG
Cuts at: 0 109 1269
Size: 109 1160
SmaI CCC'GGG
Cuts at: 0 164 1269
Size: 164 1105
SmlI C'TyrA_G
Cuts at: 0 427 925 1269
Size: 427 498 344
Fragments arranged by size:
498 427 344
SphI G_CATG'C
Cuts at: 0 624 850 1269
Size: 624 226 419
Fragments arranged by size:
624 419 226
TatI w'GTAC_w
Cuts at: 0 953 1269
Size: 953 316
Tth111I GACn'n_nGTC
Cuts at: 0 871 1269
Size: 871 398
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 636 1269
Size: 636 633
XmaI C'CCGG_G
Cuts at: 0 162 1269
Size: 162 1107
XmnI GAAnn'nnTTC
Cuts at: 0 464 1269
Size: 464 805
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlfI AlwNI ApaI
AscI AvaI BanI BanII BbeI BglI Bme1580I BmgBI
BmrI BpmI BpuEI BsaAI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssHII BstAPI BstYI BtgI BtsI EaeI EarI Eco57MI
EcoO109I FspI FspAI HaeII Hin4I HincII KasI MmeI
MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmlI
PpuMI PspOMI PsrI PstI PvuII SacII SalI SanDI
SapI SbfI SexAI SfcI SfoI SgrAI SmaI SmlI
SphI TatI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AloI
ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmtI
BplI Bpu10I BsaI BsaBI BsaWI BsaXI BsiWI BsmBI
BspEI BspHI BsrGI BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI HindIII
HpaI KpnI MfeI MluI MscI MslI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PpiI PshAI PsiI PspXI PvuI RsrII SacI ScaI
SfiI SgfI SnaBI SpeI SrfI SspI StuI StyI
SwaI TaqII TstI XbaI XhoI ZraI