Restriction map for
pF1KB8387
(Linear) (Six-base) MAPSORT of: KIBB8387.seq Check: 5882 from: 1 to: 1704
KIBB8387.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AccI GT'mk_AC
Cuts at: 0 500 1704
Size: 500 1204
Acc65I G'GTAC_C
Cuts at: 0 175 977 1704
Size: 175 802 727
Fragments arranged by size:
802 727 175
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 1085 1592 1652 1694 1704
Size: 572 513 507 60 42 10
Fragments arranged by size:
572 513 507 60 42 10
AflII C'TTAA_G
Cuts at: 0 307 346 1704
Size: 307 39 1358
Fragments arranged by size:
1358 307 39
AflIII A'CryG_T
Cuts at: 0 1683 1704
Size: 1683 21
AgeI A'CCGG_T
Cuts at: 0 781 1704
Size: 781 923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1242 1704
Size: 1242 462
ApoI r'AATT_y
Cuts at: 0 117 760 1550 1704
Size: 117 643 790 154
Fragments arranged by size:
790 643 154 117
AvaI C'yCGr_G
Cuts at: 0 1112 1704
Size: 1112 592
AvrII C'CTAG_G
Cuts at: 0 1272 1704
Size: 1272 432
BanI G'GyrC_C
Cuts at: 0 175 977 1291 1704
Size: 175 802 314 413
Fragments arranged by size:
802 413 314 175
BciVI GTATCCnnnnn_n'
Cuts at: 0 1182 1704
Size: 1182 522
BglI GCCn_nnn'nGGC
Cuts at: 0 25 1704
Size: 25 1679
BglII A'GATC_T
Cuts at: 0 1443 1704
Size: 1443 261
Bme1580I G_kGCm'C
Cuts at: 0 1294 1704
Size: 1294 410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 660 1417 1704
Size: 416 244 757 287
Fragments arranged by size:
757 416 287 244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 525 1666 1704
Size: 525 1141 38
Fragments arranged by size:
1141 525 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 1314 1576 1704
Size: 1314 262 128
Fragments arranged by size:
1314 262 128
BsaWI w'CCGG_w
Cuts at: 0 781 1704
Size: 781 923
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1619 1649 1704
Size: 1619 30 55
Fragments arranged by size:
1619 55 30
BseYI C'CCAG_C
Cuts at: 0 94 1704
Size: 94 1610
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 983 1704
Size: 983 721
BsiEI CG_ry'CG
Cuts at: 0 102 1704
Size: 102 1602
BsiWI C'GTAC_G
Cuts at: 0 896 1704
Size: 896 808
BsmI GAATG_Cn'
Cuts at: 0 1113 1315 1704
Size: 1113 202 389
Fragments arranged by size:
1113 389 202
Bsp1286I G_dGCh'C
Cuts at: 0 1294 1704
Size: 1294 410
BspHI T'CATG_A
Cuts at: 0 953 1425 1704
Size: 953 472 279
Fragments arranged by size:
953 472 279
BsrFI r'CCGG_y
Cuts at: 0 56 781 1704
Size: 56 725 923
Fragments arranged by size:
923 725 56
BstBI TT'CG_AA
Cuts at: 0 450 1704
Size: 450 1254
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1230 1446 1704
Size: 1230 216 258
Fragments arranged by size:
1230 258 216
BstYI r'GATC_y
Cuts at: 0 882 1443 1704
Size: 882 561 261
Fragments arranged by size:
882 561 261
BstZ17I GTA'TAC
Cuts at: 0 501 1704
Size: 501 1203
BtgI C'CryG_G
Cuts at: 0 23 981 1704
Size: 23 958 723
Fragments arranged by size:
958 723 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 84 1704
Size: 84 1620
DraI TTT'AAA
Cuts at: 0 759 1704
Size: 759 945
DrdI GACnn_nn'nnGTC
Cuts at: 0 467 1704
Size: 467 1237
EaeI y'GGCC_r
Cuts at: 0 14 1704
Size: 14 1690
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 572 660 1085 1417 1592 1652 1694
Size: 416 156 88 425 332 175 60 42
Cuts at: 1694 1704
Size: 10
Fragments arranged by size:
425 416 332 175 156 88 60 42
10
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 322 361 1704
Size: 322 39 1343
Fragments arranged by size:
1343 322 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 349 381 472 504 1704
Size: 349 32 91 32 1200
Fragments arranged by size:
1200 349 91 32 32
HindIII A'AGCT_T
Cuts at: 0 135 1704
Size: 135 1569
KpnI G_GTAC'C
Cuts at: 0 179 981 1704
Size: 179 802 723
Fragments arranged by size:
802 723 179
MslI CAynn'nnrTG
Cuts at: 0 1228 1704
Size: 1228 476
MspA1I CmG'CkG
Cuts at: 0 25 1704
Size: 25 1679
NaeI GCC'GGC
Cuts at: 0 58 1704
Size: 58 1646
NcoI C'CATG_G
Cuts at: 0 981 1704
Size: 981 723
NgoMIV G'CCGG_C
Cuts at: 0 56 1704
Size: 56 1648
NspI r_CATG'y
Cuts at: 0 1687 1704
Size: 1687 17
PciI A'CATG_T
Cuts at: 0 1683 1704
Size: 1683 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 172 1704
Size: 172 1532
PfoI T'CCnGG_A
Cuts at: 0 1394 1704
Size: 1394 310
PsiI TTA'TAA
Cuts at: 0 1213 1704
Size: 1213 491
PstI C_TGCA'G
Cuts at: 0 1200 1704
Size: 1200 504
SacII CC_GC'GG
Cuts at: 0 26 1704
Size: 26 1678
SfcI C'TryA_G
Cuts at: 0 156 1196 1704
Size: 156 1040 508
Fragments arranged by size:
1040 508 156
SmlI C'TyrA_G
Cuts at: 0 307 346 504 1645 1704
Size: 307 39 158 1141 59
Fragments arranged by size:
1141 307 158 59 39
SspI AAT'ATT
Cuts at: 0 632 1611 1704
Size: 632 979 93
Fragments arranged by size:
979 632 93
StyI C'CwwG_G
Cuts at: 0 981 1272 1704
Size: 981 291 432
Fragments arranged by size:
981 432 291
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 949 1273 1704
Size: 949 324 431
Fragments arranged by size:
949 431 324
TatI w'GTAC_w
Cuts at: 0 197 371 1165 1374 1704
Size: 197 174 794 209 330
Fragments arranged by size:
794 330 209 197 174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 701 733 1704
Size: 701 32 971
Fragments arranged by size:
971 701 32
Tth111I GACn'n_nGTC
Cuts at: 0 467 1704
Size: 467 1237
XbaI T'CTAG_A
Cuts at: 0 438 1704
Size: 438 1266
XmnI GAAnn'nnTTC
Cuts at: 0 138 385 446 1022 1704
Size: 138 247 61 576 682
Fragments arranged by size:
682 576 247 138 61
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AgeI AlfI ApoI
AvaI AvrII BanI BciVI BglI BglII Bme1580I BpmI
BpuEI BsaI BsaWI BsaXI BseYI BsgI BsiEI BsiWI
BsmI Bsp1286I BspHI BsrFI BstBI BstXI BstYI BstZ17I
BtgI BtgZI DraI DrdI EaeI Eco57MI FalI Hin4I
HindIII KpnI MslI MspA1I NaeI NcoI NgoMIV NspI
PciI PflMI PfoI PsiI PstI SacII SfcI SmlI
SspI StyI TaqII TatI TstI Tth111I XbaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AhdI AleI AloI AlwNI
ApaI ApaLI AscI AseI AsiSI BaeI BamHI BanII
BbeI BbsI BbvCI BcgI BclI BfrBI BlpI BmgBI
BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaHI BseRI
BsiHKAI BsmBI BspEI BspMI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstEII Bsu36I BtsI ClaI CspCI DraIII
EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FseI FspI FspAI HaeII HincII HpaI KasI MfeI
MluI MmeI MscI NarI NdeI NheI NotI NruI
NsiI PacI PasI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
XcmI XhoI XmaI ZraI