(Linear) (Six-base) MAPSORT of: KIBB8397.seq  Check: 5784  from: 1  to: 1386



KIBB8397.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AccI GT'mk_AC

Cuts at:      0     980    1386 
   Size:        980     406
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     556    1094    1386 
   Size:        556     538     292

  Fragments arranged by size: 

                 556     538     292
 
AflIII A'CryG_T

Cuts at:      0     742    1386 
   Size:        742     644
 
ApoI r'AATT_y

Cuts at:      0    1090    1312    1386 
   Size:       1090     222      74

  Fragments arranged by size: 

                1090     222      74
 
AseI AT'TA_AT

Cuts at:      0    1069    1075    1386 
   Size:       1069       6     311

  Fragments arranged by size: 

                1069     311       6
 
AvrII C'CTAG_G

Cuts at:      0     993    1386 
   Size:        993     393
 
BamHI G'GATC_C

Cuts at:      0    1149    1386 
   Size:       1149     237
 
BanI G'GyrC_C

Cuts at:      0     939    1386 
   Size:        939     447
 
BanII G_rGCy'C

Cuts at:      0    1139    1386 
   Size:       1139     247
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      94     916    1088    1386 
   Size:         94     822     172     298

  Fragments arranged by size: 

                 822     298     172      94
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      84     214     257     911    1238    1386 
   Size:         84     130      43     654     327     148

  Fragments arranged by size: 

                 654     327     148     130      84      43
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     799    1256    1386 
   Size:        110     689     457     130

  Fragments arranged by size: 

                 689     457     130     110
 
BsaWI w'CCGG_w

Cuts at:      0     790    1386 
   Size:        790     596
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     355    1386 
   Size:        355    1031
 
BseYI C'CCAG_C

Cuts at:      0    1377    1386 
   Size:       1377       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     776    1386 
   Size:        776     610
 
Bsp1286I G_dGCh'C

Cuts at:      0    1139    1386 
   Size:       1139     247
 
BspEI T'CCGG_A

Cuts at:      0     790    1386 
   Size:        790     596
 
BsrBI CCG'CTC

Cuts at:      0     720    1386 
   Size:        720     666
 
BsrDI GCAATG_nn'

Cuts at:      0    1123    1386 
   Size:       1123     263
 
BstEII G'GTnAC_C

Cuts at:      0    1028    1386 
   Size:       1028     358
 
BstYI r'GATC_y

Cuts at:      0    1149    1386 
   Size:       1149     237
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      20    1386 
   Size:         20    1366
 
DraI TTT'AAA

Cuts at:      0    1266    1386 
   Size:       1266     120
 
EaeI y'GGCC_r

Cuts at:      0     846     969    1386 
   Size:        846     123     417

  Fragments arranged by size: 

                 846     417     123
 
EarI CTCTTCn'nnn_

Cuts at:      0      34     332     386    1113    1386 
   Size:         34     298      54     727     273

  Fragments arranged by size: 

                 727     298     273      54      34
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      84     214     257     556     911    1094    1238    1386 
   Size:         84     130      43     299     355     183     144     148

  Fragments arranged by size: 

                 355     299     183     148     144     130      84      43
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     990    1386 
   Size:        990     396
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1105    1134    1137    1166    1386 
   Size:       1105      29       3      29     220

  Fragments arranged by size: 

                1105     220      29      29       3
 
HindIII A'AGCT_T

Cuts at:      0    1249    1386 
   Size:       1249     137
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     385     800    1386 
   Size:        385     415     586

  Fragments arranged by size: 

                 586     415     385
 
MscI TGG'CCA

Cuts at:      0     848    1386 
   Size:        848     538
 
NspI r_CATG'y

Cuts at:      0    1377    1386 
   Size:       1377       9
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     709     741    1386 
   Size:        709      32     645

  Fragments arranged by size: 

                 709     645      32
 
PsiI TTA'TAA

Cuts at:      0     217    1386 
   Size:        217    1169
 
PstI C_TGCA'G

Cuts at:      0     329    1386 
   Size:        329    1057
 
SfcI C'TryA_G

Cuts at:      0     311     325    1386 
   Size:        311      14    1061

  Fragments arranged by size: 

                1061     311      14
 
SmlI C'TyrA_G

Cuts at:      0     125     814    1271    1386 
   Size:        125     689     457     115

  Fragments arranged by size: 

                 689     457     125     115
 
SphI G_CATG'C

Cuts at:      0    1377    1386 
   Size:       1377       9
 
SspI AAT'ATT

Cuts at:      0     637    1073    1386 
   Size:        637     436     313

  Fragments arranged by size: 

                 637     436     313
 
StyI C'CwwG_G

Cuts at:      0     773     993    1164    1386 
   Size:        773     220     171     222

  Fragments arranged by size: 

                 773     222     220     171
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     597     629     785     817    1386 
   Size:        597      32     156      32     569

  Fragments arranged by size: 

                 597     569     156      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     913    1386 
   Size:        913     473
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     ApoI     AseI    AvrII    BamHI     BanI
    BanII     BbsI     BpmI    BpuEI    BsaWI    BseRI    BseYI     BsgI
 Bsp1286I    BspEI    BsrBI    BsrDI   BstEII    BstYI    BtgZI     DraI
     EaeI     EarI  Eco57MI EcoO109I    Hin4I  HindIII     MmeI     MscI
     NspI     PpiI     PsiI     PstI     SfcI     SmlI     SphI     SspI
     StyI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI     BaeI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     BtgI     BtsI
     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     StuI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI