Restriction map for
pF1KB8398
(Linear) (Six-base) MAPSORT of: KIBB8398.seq Check: 7321 from: 1 to: 1884
KIBB8398.orf
With 182 enzymes: SgfI *
February 20, 2009 16:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1457 1884
Size: 1457 427
AflIII A'CryG_T
Cuts at: 0 1427 1884
Size: 1427 457
AhdI GACnn_n'nnGTC
Cuts at: 0 699 1884
Size: 699 1185
AleI CACnn'nnGTG
Cuts at: 0 928 1884
Size: 928 956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 631 1884
Size: 631 1253
AlwNI CAG_nnn'CTG
Cuts at: 0 899 1252 1624 1860 1884
Size: 899 353 372 236 24
Fragments arranged by size:
899 372 353 236 24
ApaI G_GGCC'C
Cuts at: 0 1762 1884
Size: 1762 122
ApaLI G'TGCA_C
Cuts at: 0 487 1884
Size: 487 1397
AvaI C'yCGr_G
Cuts at: 0 219 926 1387 1601 1884
Size: 219 707 461 214 283
Fragments arranged by size:
707 461 283 219 214
BamHI G'GATC_C
Cuts at: 0 1443 1791 1884
Size: 1443 348 93
Fragments arranged by size:
1443 348 93
BanI G'GyrC_C
Cuts at: 0 125 149 184 224 736 962 1884
Size: 125 24 35 40 512 226 922
Fragments arranged by size:
922 512 226 125 40 35 24
BanII G_rGCy'C
Cuts at: 0 220 637 1286 1306 1394 1412 1544 1697
Size: 220 417 649 20 88 18 132 153
Cuts at: 1697 1748 1762 1884
Size: 51 14 122
Fragments arranged by size:
649 417 220 153 132 122 88 51
20 18 14
BbeI G_GCGC'C
Cuts at: 0 228 1884
Size: 228 1656
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 354 388 1884
Size: 354 34 1496
Fragments arranged by size:
1496 354 34
BclI T'GATC_A
Cuts at: 0 80 1884
Size: 80 1804
BglI GCCn_nnn'nGGC
Cuts at: 0 994 1683 1884
Size: 994 689 201
Fragments arranged by size:
994 689 201
BglII A'GATC_T
Cuts at: 0 276 522 1884
Size: 276 246 1362
Fragments arranged by size:
1362 276 246
BlpI GC'TnA_GC
Cuts at: 0 1285 1884
Size: 1285 599
Bme1580I G_kGCm'C
Cuts at: 0 491 965 1762 1884
Size: 491 474 797 122
Fragments arranged by size:
797 491 474 122
BmgBI CAC'GTC
Cuts at: 0 1428 1884
Size: 1428 456
BmrI ACTGGGnnnn_n'
Cuts at: 0 921 1146 1388 1884
Size: 921 225 242 496
Fragments arranged by size:
921 496 242 225
BmtI G_CTAG'C
Cuts at: 0 58 1884
Size: 58 1826
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1767 1884
Size: 1767 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 553 901 1051 1123 1884
Size: 187 366 348 150 72 761
Fragments arranged by size:
761 366 348 187 150 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 1105 1195 1216 1330 1759 1884
Size: 511 594 90 21 114 429 125
Fragments arranged by size:
594 511 429 125 114 90 21
BsaI GGTCTCn'nnnn_
Cuts at: 0 163 1884
Size: 163 1721
BsaBI GATnn'nnATC
Cuts at: 0 304 376 1884
Size: 304 72 1508
Fragments arranged by size:
1508 304 72
BsaHI Gr'CG_yC
Cuts at: 0 225 1884
Size: 225 1659
BsaWI w'CCGG_w
Cuts at: 0 248 839 1278 1884
Size: 248 591 439 606
Fragments arranged by size:
606 591 439 248
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 706 1371 1474 1756 1884
Size: 706 665 103 282 128
Fragments arranged by size:
706 665 282 128 103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1071 1884
Size: 1071 813
BsiEI CG_ry'CG
Cuts at: 0 47 64 1884
Size: 47 17 1820
Fragments arranged by size:
1820 47 17
BsiHKAI G_wGCw'C
Cuts at: 0 220 491 1286 1412 1544 1884
Size: 220 271 795 126 132 340
Fragments arranged by size:
795 340 271 220 132 126
BsmI GAATG_Cn'
Cuts at: 0 1113 1226 1340 1884
Size: 1113 113 114 544
Fragments arranged by size:
1113 544 114 113
BsmBI CGTCTCn'nnnn_
Cuts at: 0 483 1884
Size: 483 1401
Bsp1286I G_dGCh'C
Cuts at: 0 220 491 637 965 1286 1306 1394 1412
Size: 220 271 146 328 321 20 88 18
Cuts at: 1412 1544 1697 1748 1762 1884
Size: 132 153 51 14 122
Fragments arranged by size:
328 321 271 220 153 146 132 122
88 51 20 18 14
BspEI T'CCGG_A
Cuts at: 0 248 1278 1884
Size: 248 1030 606
Fragments arranged by size:
1030 606 248
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 152 1884
Size: 152 1732
BsrBI CCG'CTC
Cuts at: 0 429 1884
Size: 429 1455
BsrDI GCAATG_nn'
Cuts at: 0 335 1884
Size: 335 1549
BstAPI GCAn_nnn'nTGC
Cuts at: 0 144 1884
Size: 144 1740
BstXI CCAn_nnnn'nTGG
Cuts at: 0 930 1195 1884
Size: 930 265 689
Fragments arranged by size:
930 689 265
BstYI r'GATC_y
Cuts at: 0 276 522 767 1443 1791 1884
Size: 276 246 245 676 348 93
Fragments arranged by size:
676 348 276 246 245 93
Bsu36I CC'TnA_GG
Cuts at: 0 1617 1774 1884
Size: 1617 157 110
Fragments arranged by size:
1617 157 110
BtgI C'CryG_G
Cuts at: 0 992 1884
Size: 992 892
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1174 1474 1884
Size: 1174 300 410
Fragments arranged by size:
1174 410 300
BtsI GCAGTG_nn'
Cuts at: 0 978 1181 1699 1884
Size: 978 203 518 185
Fragments arranged by size:
978 518 203 185
DraIII CAC_nnn'GTG
Cuts at: 0 1078 1884
Size: 1078 806
EaeI y'GGCC_r
Cuts at: 0 44 61 233 890 1884
Size: 44 17 172 657 994
Fragments arranged by size:
994 657 172 44 17
EagI C'GGCC_G
Cuts at: 0 44 61 1884
Size: 44 17 1823
Fragments arranged by size:
1823 44 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 85 1884
Size: 85 1799
EcoICRI GAG'CTC
Cuts at: 0 218 1284 1410 1542 1884
Size: 218 1066 126 132 342
Fragments arranged by size:
1066 342 218 132 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 553 901 1051 1123 1457 1884
Size: 187 366 348 150 72 334 427
Fragments arranged by size:
427 366 348 334 187 150 72
EcoO109I rG'GnC_Cy
Cuts at: 0 794 1272 1758 1759 1884
Size: 794 478 486 1 125
Fragments arranged by size:
794 486 478 125 1
HaeII r_GCGC'y
Cuts at: 0 27 228 1110 1782 1884
Size: 27 201 882 672 102
Fragments arranged by size:
882 672 201 102 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 295 327 685 717 1428 1460 1884
Size: 295 32 358 32 711 32 424
Fragments arranged by size:
711 424 358 295 32 32 32
HincII GTy'rAC
Cuts at: 0 909 1884
Size: 909 975
HindIII A'AGCT_T
Cuts at: 0 1469 1884
Size: 1469 415
KasI G'GCGC_C
Cuts at: 0 224 1884
Size: 224 1660
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 614 1884
Size: 614 1270
MscI TGG'CCA
Cuts at: 0 235 892 1884
Size: 235 657 992
Fragments arranged by size:
992 657 235
MslI CAynn'nnrTG
Cuts at: 0 727 928 956 1884
Size: 727 201 28 928
Fragments arranged by size:
928 727 201 28
MspA1I CmG'CkG
Cuts at: 0 66 698 1323 1668 1808 1884
Size: 66 632 625 345 140 76
Fragments arranged by size:
632 625 345 140 76 66
NarI GG'CG_CC
Cuts at: 0 225 1884
Size: 225 1659
NcoI C'CATG_G
Cuts at: 0 992 1884
Size: 992 892
NheI G'CTAG_C
Cuts at: 0 54 1884
Size: 54 1830
NspI r_CATG'y
Cuts at: 0 1072 1884
Size: 1072 812
PasI CC'CwG_GG
Cuts at: 0 174 705 1306 1884
Size: 174 531 601 578
Fragments arranged by size:
601 578 531 174
PflMI CCAn_nnn'nTGG
Cuts at: 0 899 1884
Size: 899 985
PfoI T'CCnGG_A
Cuts at: 0 164 1028 1884
Size: 164 864 856
Fragments arranged by size:
864 856 164
PpuMI rG'GwC_Cy
Cuts at: 0 1272 1884
Size: 1272 612
PspOMI G'GGCC_C
Cuts at: 0 1758 1884
Size: 1758 126
PstI C_TGCA'G
Cuts at: 0 620 1257 1884
Size: 620 637 627
Fragments arranged by size:
637 627 620
PvuII CAG'CTG
Cuts at: 0 698 1668 1808 1884
Size: 698 970 140 76
Fragments arranged by size:
970 698 140 76
SacI G_AGCT'C
Cuts at: 0 220 1286 1412 1544 1884
Size: 220 1066 126 132 340
Fragments arranged by size:
1066 340 220 132 126
SexAI A'CCwGG_T
Cuts at: 0 1074 1884
Size: 1074 810
SfcI C'TryA_G
Cuts at: 0 616 1253 1884
Size: 616 637 631
Fragments arranged by size:
637 631 616
SfoI GGC'GCC
Cuts at: 0 226 1884
Size: 226 1658
SmaI CCC'GGG
Cuts at: 0 1603 1884
Size: 1603 281
SmlI C'TyrA_G
Cuts at: 0 526 1120 1210 1231 1345 1738 1884
Size: 526 594 90 21 114 393 146
Fragments arranged by size:
594 526 393 146 114 90 21
SphI G_CATG'C
Cuts at: 0 1072 1884
Size: 1072 812
SrfI GCCC'GGGC
Cuts at: 0 1603 1884
Size: 1603 281
StyI C'CwwG_G
Cuts at: 0 992 1043 1448 1535 1884
Size: 992 51 405 87 349
Fragments arranged by size:
992 405 349 87 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 697 1884
Size: 697 1187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1098 1884
Size: 1098 786
XmaI C'CCGG_G
Cuts at: 0 1601 1884
Size: 1601 283
Enzymes that do cut:
AcuI AflIII AhdI AleI AlfI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbeI BcgI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
BpuEI BsaI BsaBI BsaHI BsaWI BseRI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII
EaeI EagI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I
HincII HindIII KasI MmeI MscI MslI MspA1I NarI
NcoI NheI NspI PasI PflMI PfoI PpuMI PspOMI
PstI PvuII SacI SexAI SfcI SfoI SmaI SmlI
SphI SrfI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AloI ApoI AscI AseI AsiSI AvrII BaeI BbsI
BbvCI BciVI BfrBI Bpu10I BsaAI BsaXI BseYI BsiWI
BspHI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I
ClaI CspCI DraI DrdI EarI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HpaI KpnI MfeI MluI
NaeI NdeI NgoMIV NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SfiI
SgfI SgrAI SnaBI SpeI SspI StuI SwaI TatI
TstI Tth111I XbaI XhoI XmnI ZraI