Restriction map for
pF1KB8406
(Linear) (Six-base) MAPSORT of: KIBB8406.seq Check: 4664 from: 1 to: 783
KIBB8406.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 606 690 783
Size: 438 168 84 93
Fragments arranged by size:
438 168 93 84
AlwNI CAG_nnn'CTG
Cuts at: 0 473 783
Size: 473 310
ApoI r'AATT_y
Cuts at: 0 28 68 308 783
Size: 28 40 240 475
Fragments arranged by size:
475 240 40 28
BanII G_rGCy'C
Cuts at: 0 298 783
Size: 298 485
BfrBI ATG'CAT
Cuts at: 0 60 396 480 783
Size: 60 336 84 303
Fragments arranged by size:
336 303 84 60
BglII A'GATC_T
Cuts at: 0 48 783
Size: 48 735
Bme1580I G_kGCm'C
Cuts at: 0 16 783
Size: 16 767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 450 602 783
Size: 450 152 181
Fragments arranged by size:
450 181 152
BsaI GGTCTCn'nnnn_
Cuts at: 0 146 783
Size: 146 637
BsaWI w'CCGG_w
Cuts at: 0 412 783
Size: 412 371
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 155 185 783
Size: 155 30 598
Fragments arranged by size:
598 155 30
BsiHKAI G_wGCw'C
Cuts at: 0 298 783
Size: 298 485
Bsp1286I G_dGCh'C
Cuts at: 0 16 298 783
Size: 16 282 485
Fragments arranged by size:
485 282 16
BstYI r'GATC_y
Cuts at: 0 48 783
Size: 48 735
DraI TTT'AAA
Cuts at: 0 429 783
Size: 429 354
DraIII CAC_nnn'GTG
Cuts at: 0 246 783
Size: 246 537
EarI CTCTTCn'nnn_
Cuts at: 0 431 783
Size: 431 352
EcoICRI GAG'CTC
Cuts at: 0 296 783
Size: 296 487
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 450 602 606 690 783
Size: 438 12 152 4 84 93
Fragments arranged by size:
438 152 93 84 12 4
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 135 783
Size: 135 648
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 33 65 155 187 285 317 747 779
Size: 33 32 90 32 98 32 430 32
Cuts at: 779 783
Size: 4
Fragments arranged by size:
430 98 90 33 32 32 32 32
4
MfeI C'AATT_G
Cuts at: 0 288 783
Size: 288 495
MslI CAynn'nnrTG
Cuts at: 0 95 329 783
Size: 95 234 454
Fragments arranged by size:
454 234 95
MspA1I CmG'CkG
Cuts at: 0 79 783
Size: 79 704
NdeI CA'TA_TG
Cuts at: 0 314 783
Size: 314 469
NsiI A_TGCA'T
Cuts at: 0 62 398 482 783
Size: 62 336 84 301
Fragments arranged by size:
336 301 84 62
NspI r_CATG'y
Cuts at: 0 396 480 783
Size: 396 84 303
Fragments arranged by size:
396 303 84
PflMI CCAn_nnn'nTGG
Cuts at: 0 246 783
Size: 246 537
PvuII CAG'CTG
Cuts at: 0 79 783
Size: 79 704
SacI G_AGCT'C
Cuts at: 0 298 783
Size: 298 485
SfcI C'TryA_G
Cuts at: 0 348 594 783
Size: 348 246 189
Fragments arranged by size:
348 246 189
SphI G_CATG'C
Cuts at: 0 396 480 783
Size: 396 84 303
Fragments arranged by size:
396 303 84
StuI AGG'CCT
Cuts at: 0 451 619 703 783
Size: 451 168 84 80
Fragments arranged by size:
451 168 84 80
XmnI GAAnn'nnTTC
Cuts at: 0 451 535 619 676 783
Size: 451 84 84 57 107
Fragments arranged by size:
451 107 84 84 57
Enzymes that do cut:
AcuI AlwNI ApoI BanII BfrBI BglII Bme1580I BpmI
BsaI BsaWI BsaXI BsiHKAI Bsp1286I BstYI DraI DraIII
EarI EcoICRI Eco57MI FalI Hin4I MfeI MslI MspA1I
NdeI NsiI NspI PflMI PvuII SacI SfcI SphI
StuI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BclI BglI BlpI BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI
BseRI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DrdI EaeI EagI EciI EcoNI
EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MluI MmeI MscI NaeI
NarI NcoI NgoMIV NheI NotI NruI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI