Restriction map for
pF1KB8407
(Linear) (Six-base) MAPSORT of: KIBB8407.seq Check: 9208 from: 1 to: 882
KIBB8407.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 882
Size: 511 371
AflIII A'CryG_T
Cuts at: 0 727 882
Size: 727 155
AleI CACnn'nnGTG
Cuts at: 0 508 882
Size: 508 374
AlwNI CAG_nnn'CTG
Cuts at: 0 264 566 882
Size: 264 302 316
Fragments arranged by size:
316 302 264
AscI GG'CGCG_CC
Cuts at: 0 121 882
Size: 121 761
BanI G'GyrC_C
Cuts at: 0 148 882
Size: 148 734
BbsI GAAGACnn'nnnn_
Cuts at: 0 472 882
Size: 472 410
BciVI GTATCCnnnnn_n'
Cuts at: 0 17 882
Size: 17 865
Bme1580I G_kGCm'C
Cuts at: 0 153 882
Size: 153 729
BmrI ACTGGGnnnn_n'
Cuts at: 0 213 441 882
Size: 213 228 441
Fragments arranged by size:
441 228 213
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 882
Size: 97 785
BsaI GGTCTCn'nnnn_
Cuts at: 0 631 882
Size: 631 251
BsaWI w'CCGG_w
Cuts at: 0 145 882
Size: 145 737
BseYI C'CCAG_C
Cuts at: 0 152 882
Size: 152 730
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 882
Size: 46 836
BsiEI CG_ry'CG
Cuts at: 0 50 882
Size: 50 832
BsiHKAI G_wGCw'C
Cuts at: 0 182 882
Size: 182 700
Bsp1286I G_dGCh'C
Cuts at: 0 153 182 882
Size: 153 29 700
Fragments arranged by size:
700 153 29
BsrBI CCG'CTC
Cuts at: 0 46 882
Size: 46 836
BsrFI r'CCGG_y
Cuts at: 0 15 156 882
Size: 15 141 726
Fragments arranged by size:
726 141 15
BssHII G'CGCG_C
Cuts at: 0 121 882
Size: 121 761
BstAPI GCAn_nnn'nTGC
Cuts at: 0 566 882
Size: 566 316
BstXI CCAn_nnnn'nTGG
Cuts at: 0 510 882
Size: 510 372
BtgI C'CryG_G
Cuts at: 0 20 32 321 882
Size: 20 12 289 561
Fragments arranged by size:
561 289 20 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 791 826 882
Size: 791 35 56
Fragments arranged by size:
791 56 35
DraIII CAC_nnn'GTG
Cuts at: 0 221 882
Size: 221 661
DrdI GACnn_nn'nnGTC
Cuts at: 0 685 882
Size: 685 197
EaeI y'GGCC_r
Cuts at: 0 17 47 882
Size: 17 30 835
Fragments arranged by size:
835 30 17
EagI C'GGCC_G
Cuts at: 0 47 882
Size: 47 835
EarI CTCTTCn'nnn_
Cuts at: 0 211 298 367 882
Size: 211 87 69 515
Fragments arranged by size:
515 211 87 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 56 691 882
Size: 56 635 191
Fragments arranged by size:
635 191 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 511 882
Size: 97 414 371
Fragments arranged by size:
414 371 97
MslI CAynn'nnrTG
Cuts at: 0 508 882
Size: 508 374
MspA1I CmG'CkG
Cuts at: 0 34 703 776 882
Size: 34 669 73 106
Fragments arranged by size:
669 106 73 34
NaeI GCC'GGC
Cuts at: 0 17 882
Size: 17 865
NcoI C'CATG_G
Cuts at: 0 321 882
Size: 321 561
NgoMIV G'CCGG_C
Cuts at: 0 15 882
Size: 15 867
NspI r_CATG'y
Cuts at: 0 292 731 882
Size: 292 439 151
Fragments arranged by size:
439 292 151
PasI CC'CwG_GG
Cuts at: 0 822 882
Size: 822 60
PciI A'CATG_T
Cuts at: 0 727 882
Size: 727 155
PflMI CCAn_nnn'nTGG
Cuts at: 0 159 264 327 882
Size: 159 105 63 555
Fragments arranged by size:
555 159 105 63
PstI C_TGCA'G
Cuts at: 0 562 882
Size: 562 320
PvuII CAG'CTG
Cuts at: 0 703 882
Size: 703 179
SacII CC_GC'GG
Cuts at: 0 35 882
Size: 35 847
SapI GCTCTTCn'nnn_
Cuts at: 0 367 882
Size: 367 515
SfcI C'TryA_G
Cuts at: 0 558 882
Size: 558 324
StyI C'CwwG_G
Cuts at: 0 321 872 882
Size: 321 551 10
Fragments arranged by size:
551 321 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 232 882
Size: 232 650
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 681 713 882
Size: 681 32 169
Fragments arranged by size:
681 169 32
Tth111I GACn'n_nGTC
Cuts at: 0 589 882
Size: 589 293
Enzymes that do cut:
AcuI AflIII AleI AlwNI AscI BanI BbsI BciVI
Bme1580I BmrI BpmI BsaI BsaWI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BsrBI BsrFI BssHII BstAPI BstXI BtgI
CspCI DraIII DrdI EaeI EagI EarI EciI Eco57MI
MslI MspA1I NaeI NcoI NgoMIV NspI PasI PciI
PflMI PstI PvuII SacII SapI SfcI StyI TaqII
TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbvCI BcgI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssSI
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
DraI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI NarI NdeI NheI
NotI NruI NsiI PacI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI XbaI XcmI XhoI XmaI
XmnI ZraI