Restriction map for
pF1KB8410
(Linear) (Six-base) MAPSORT of: KIBB8410.seq Check: 5587 from: 1 to: 948
KIBB8410.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AflIII A'CryG_T
Cuts at: 0 396 948
Size: 396 552
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 308 948
Size: 308 640
AlwNI CAG_nnn'CTG
Cuts at: 0 187 948
Size: 187 761
ApoI r'AATT_y
Cuts at: 0 151 272 948
Size: 151 121 676
Fragments arranged by size:
676 151 121
AvaI C'yCGr_G
Cuts at: 0 40 948
Size: 40 908
BamHI G'GATC_C
Cuts at: 0 746 948
Size: 746 202
BanII G_rGCy'C
Cuts at: 0 580 948
Size: 580 368
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 564 594 892 948
Size: 263 301 30 298 56
Fragments arranged by size:
301 298 263 56 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 739 948
Size: 739 209
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 37 46 837 948
Size: 28 9 9 791 111
Fragments arranged by size:
791 111 28 9 9
BsiHKAI G_wGCw'C
Cuts at: 0 85 580 948
Size: 85 495 368
Fragments arranged by size:
495 368 85
Bsp1286I G_dGCh'C
Cuts at: 0 85 580 948
Size: 85 495 368
Fragments arranged by size:
495 368 85
BssSI C'ACGA_G
Cuts at: 0 563 948
Size: 563 385
BstAPI GCAn_nnn'nTGC
Cuts at: 0 15 24 948
Size: 15 9 924
Fragments arranged by size:
924 15 9
BstYI r'GATC_y
Cuts at: 0 710 746 782 948
Size: 710 36 36 166
Fragments arranged by size:
710 166 36 36
EaeI y'GGCC_r
Cuts at: 0 495 948
Size: 495 453
EarI CTCTTCn'nnn_
Cuts at: 0 608 911 948
Size: 608 303 37
Fragments arranged by size:
608 303 37
EcoICRI GAG'CTC
Cuts at: 0 578 948
Size: 578 370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 564 594 892 948
Size: 263 301 30 298 56
Fragments arranged by size:
301 298 263 56 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 718 948
Size: 718 230
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 700 948
Size: 700 248
FspI TGC'GCA
Cuts at: 0 178 948
Size: 178 770
FspAI rTGC'GCAy
Cuts at: 0 178 948
Size: 178 770
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 274 306 420 452 948
Size: 274 32 114 32 496
Fragments arranged by size:
496 274 114 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 839 948
Size: 839 109
MslI CAynn'nnrTG
Cuts at: 0 401 948
Size: 401 547
MspA1I CmG'CkG
Cuts at: 0 288 839 948
Size: 288 551 109
Fragments arranged by size:
551 288 109
NspI r_CATG'y
Cuts at: 0 400 948
Size: 400 548
PciI A'CATG_T
Cuts at: 0 396 948
Size: 396 552
PflMI CCAn_nnn'nTGG
Cuts at: 0 432 948
Size: 432 516
PstI C_TGCA'G
Cuts at: 0 297 948
Size: 297 651
PvuII CAG'CTG
Cuts at: 0 288 948
Size: 288 660
SacI G_AGCT'C
Cuts at: 0 580 948
Size: 580 368
SapI GCTCTTCn'nnn_
Cuts at: 0 911 948
Size: 911 37
SfcI C'TryA_G
Cuts at: 0 293 850 948
Size: 293 557 98
Fragments arranged by size:
557 293 98
SmlI C'TyrA_G
Cuts at: 0 40 948
Size: 40 908
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 278 948
Size: 278 670
XbaI T'CTAG_A
Cuts at: 0 942 948
Size: 942 6
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 348 569 948
Size: 348 221 379
Fragments arranged by size:
379 348 221
XhoI C'TCGA_G
Cuts at: 0 40 948
Size: 40 908
Enzymes that do cut:
AflIII AlfI AlwNI ApoI AvaI BamHI BanII BpmI
BseRI BsgI BsiHKAI Bsp1286I BssSI BstAPI BstYI EaeI
EarI EcoICRI Eco57MI EcoNI FalI FspI FspAI Hin4I
MmeI MslI MspA1I NspI PciI PflMI PstI PvuII
SacI SapI SfcI SmlI TaqII XbaI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoO109I
EcoRI EcoRV FseI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TatI TstI
Tth111I XmaI XmnI ZraI