Restriction map for
pF1KB8411
(Linear) (Six-base) MAPSORT of: KIBB8411.seq Check: 7270 from: 1 to: 2598
KIBB8411.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 2075 2231 2273 2598
Size: 617 1458 156 42 325
Fragments arranged by size:
1458 617 325 156 42
AfeI AGC'GCT
Cuts at: 0 1105 2598
Size: 1105 1493
AflIII A'CryG_T
Cuts at: 0 1693 2598
Size: 1693 905
AleI CACnn'nnGTG
Cuts at: 0 2030 2598
Size: 2030 568
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 842 2598
Size: 842 1756
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 412 444 2598
Size: 412 32 2154
Fragments arranged by size:
2154 412 32
AlwNI CAG_nnn'CTG
Cuts at: 0 540 1251 2373 2598
Size: 540 711 1122 225
Fragments arranged by size:
1122 711 540 225
ApaI G_GGCC'C
Cuts at: 0 92 644 1179 2598
Size: 92 552 535 1419
Fragments arranged by size:
1419 552 535 92
ApoI r'AATT_y
Cuts at: 0 226 259 631 1561 2598
Size: 226 33 372 930 1037
Fragments arranged by size:
1037 930 372 226 33
AseI AT'TA_AT
Cuts at: 0 2120 2598
Size: 2120 478
AvaI C'yCGr_G
Cuts at: 0 2352 2598
Size: 2352 246
BanI G'GyrC_C
Cuts at: 0 1811 2086 2598
Size: 1811 275 512
Fragments arranged by size:
1811 512 275
BanII G_rGCy'C
Cuts at: 0 92 644 797 848 1179 1190 1670 2166
Size: 92 552 153 51 331 11 480 496
Cuts at: 2166 2598
Size: 432
Fragments arranged by size:
552 496 480 432 331 153 92 51
11
BbsI GAAGACnn'nnnn_
Cuts at: 0 440 512 734 1001 1484 2598
Size: 440 72 222 267 483 1114
Fragments arranged by size:
1114 483 440 267 222 72
BbvCI CC'TCA_GC
Cuts at: 0 152 698 2598
Size: 152 546 1900
Fragments arranged by size:
1900 546 152
BclI T'GATC_A
Cuts at: 0 489 2598
Size: 489 2109
BglI GCCn_nnn'nGGC
Cuts at: 0 1639 2598
Size: 1639 959
BlpI GC'TnA_GC
Cuts at: 0 1669 2598
Size: 1669 929
Bme1580I G_kGCm'C
Cuts at: 0 92 644 1179 1816 2091 2495 2598
Size: 92 552 535 637 275 404 103
Fragments arranged by size:
637 552 535 404 275 103 92
BmgBI CAC'GTC
Cuts at: 0 1405 2598
Size: 1405 1193
BmrI ACTGGGnnnn_n'
Cuts at: 0 616 1010 2598
Size: 616 394 1588
Fragments arranged by size:
1588 616 394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1738 2332 2569 2598
Size: 1738 594 237 29
Fragments arranged by size:
1738 594 237 29
Bpu10I CC'TnA_GC
Cuts at: 0 152 698 2598
Size: 152 546 1900
Fragments arranged by size:
1900 546 152
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 773 2598
Size: 773 1825
BsaAI yAC'GTr
Cuts at: 0 1694 2490 2598
Size: 1694 796 108
Fragments arranged by size:
1694 796 108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 132 162 1394 1424 2598
Size: 132 30 1232 30 1174
Fragments arranged by size:
1232 1174 132 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 99 140 686 1120 1588 1786 1789 2598
Size: 99 41 546 434 468 198 3 809
Fragments arranged by size:
809 546 468 434 198 99 41 3
BseYI C'CCAG_C
Cuts at: 0 144 665 2478 2579 2598
Size: 144 521 1813 101 19
Fragments arranged by size:
1813 521 144 101 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1165 1674 2353 2598
Size: 1165 509 679 245
Fragments arranged by size:
1165 679 509 245
BsiHKAI G_wGCw'C
Cuts at: 0 848 1154 1190 1410 1670 1730 2555 2598
Size: 848 306 36 220 260 60 825 43
Fragments arranged by size:
848 825 306 260 220 60 43 36
BsiWI C'GTAC_G
Cuts at: 0 2486 2598
Size: 2486 112
Bsp1286I G_dGCh'C
Cuts at: 0 92 644 797 848 1154 1179 1190 1410
Size: 92 552 153 51 306 25 11 220
Cuts at: 1410 1670 1730 1816 2091 2166 2495 2555 2598
Size: 260 60 86 275 75 329 60 43
Fragments arranged by size:
552 329 306 275 260 220 153 92
86 75 60 60 51 43 25 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2381 2567 2598
Size: 2381 186 31
Fragments arranged by size:
2381 186 31
BsrBI CCG'CTC
Cuts at: 0 2153 2598
Size: 2153 445
BssSI C'ACGA_G
Cuts at: 0 1183 2598
Size: 1183 1415
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2075 2373 2559 2598
Size: 2075 298 186 39
Fragments arranged by size:
2075 298 186 39
BstEII G'GTnAC_C
Cuts at: 0 138 2598
Size: 138 2460
BstXI CCAn_nnnn'nTGG
Cuts at: 0 673 1716 2032 2598
Size: 673 1043 316 566
Fragments arranged by size:
1043 673 566 316
BstYI r'GATC_y
Cuts at: 0 234 1651 2598
Size: 234 1417 947
Fragments arranged by size:
1417 947 234
Bsu36I CC'TnA_GG
Cuts at: 0 581 2598
Size: 581 2017
BtgI C'CryG_G
Cuts at: 0 2584 2598
Size: 2584 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2575 2598
Size: 2575 23
BtsI GCAGTG_nn'
Cuts at: 0 48 1591 1878 2598
Size: 48 1543 287 720
Fragments arranged by size:
1543 720 287 48
DrdI GACnn_nn'nnGTC
Cuts at: 0 1804 2598
Size: 1804 794
EaeI y'GGCC_r
Cuts at: 0 276 1031 1136 1527 2598
Size: 276 755 105 391 1071
Fragments arranged by size:
1071 755 391 276 105
EarI CTCTTCn'nnn_
Cuts at: 0 17 161 696 749 1276 1849 1940 2249
Size: 17 144 535 53 527 573 91 309
Cuts at: 2249 2598
Size: 349
Fragments arranged by size:
573 535 527 349 309 144 91 53
17
EcoICRI GAG'CTC
Cuts at: 0 846 1188 1668 2598
Size: 846 342 480 930
Fragments arranged by size:
930 846 480 342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 1738 2075 2231 2273 2332 2569 2598
Size: 617 1121 337 156 42 59 237 29
Fragments arranged by size:
1121 617 337 237 156 59 42 29
EcoO109I rG'GnC_Cy
Cuts at: 0 171 640 685 1176 1391 2346 2470 2598
Size: 171 469 45 491 215 955 124 128
Fragments arranged by size:
955 491 469 215 171 128 124 45
EcoRI G'AATT_C
Cuts at: 0 259 631 2598
Size: 259 372 1967
Fragments arranged by size:
1967 372 259
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 400 422 2598
Size: 400 22 2176
Fragments arranged by size:
2176 400 22
FspI TGC'GCA
Cuts at: 0 1590 2598
Size: 1590 1008
HaeII r_GCGC'y
Cuts at: 0 1107 2598
Size: 1107 1491
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 439 471 1394 1426 1527 1559 2177 2209
Size: 439 32 923 32 101 32 618 32
Cuts at: 2209 2598
Size: 389
Fragments arranged by size:
923 618 439 389 101 32 32 32
32
HincII GTy'rAC
Cuts at: 0 339 2517 2598
Size: 339 2178 81
Fragments arranged by size:
2178 339 81
HindIII A'AGCT_T
Cuts at: 0 2224 2598
Size: 2224 374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1643 1820 2598
Size: 1643 177 778
Fragments arranged by size:
1643 778 177
MscI TGG'CCA
Cuts at: 0 278 1033 1529 2598
Size: 278 755 496 1069
Fragments arranged by size:
1069 755 496 278
MslI CAynn'nnrTG
Cuts at: 0 2030 2539 2598
Size: 2030 509 59
Fragments arranged by size:
2030 509 59
MspA1I CmG'CkG
Cuts at: 0 475 1420 1533 1673 1688 1865 2081 2310
Size: 475 945 113 140 15 177 216 229
Cuts at: 2310 2586 2598
Size: 276 12
Fragments arranged by size:
945 475 276 229 216 177 140 113
15 12
NspI r_CATG'y
Cuts at: 0 2073 2544 2598
Size: 2073 471 54
Fragments arranged by size:
2073 471 54
PasI CC'CwG_GG
Cuts at: 0 637 2598
Size: 637 1961
PfoI T'CCnGG_A
Cuts at: 0 246 2598
Size: 246 2352
PmlI CAC'GTG
Cuts at: 0 1694 2598
Size: 1694 904
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1423 1455 2598
Size: 1423 32 1143
Fragments arranged by size:
1423 1143 32
PpuMI rG'GwC_Cy
Cuts at: 0 171 1391 2598
Size: 171 1220 1207
Fragments arranged by size:
1220 1207 171
PshAI GACnn'nnGTC
Cuts at: 0 2528 2598
Size: 2528 70
PsiI TTA'TAA
Cuts at: 0 1009 2598
Size: 1009 1589
PspOMI G'GGCC_C
Cuts at: 0 88 640 1175 2598
Size: 88 552 535 1423
Fragments arranged by size:
1423 552 535 88
PstI C_TGCA'G
Cuts at: 0 831 2598
Size: 831 1767
PvuII CAG'CTG
Cuts at: 0 475 1420 1533 1673 1688 1865 2598
Size: 475 945 113 140 15 177 733
Fragments arranged by size:
945 733 475 177 140 113 15
SacI G_AGCT'C
Cuts at: 0 848 1190 1670 2598
Size: 848 342 480 928
Fragments arranged by size:
928 848 480 342
SacII CC_GC'GG
Cuts at: 0 2587 2598
Size: 2587 11
SapI GCTCTTCn'nnn_
Cuts at: 0 749 1940 2598
Size: 749 1191 658
Fragments arranged by size:
1191 749 658
ScaI AGT'ACT
Cuts at: 0 1753 2598
Size: 1753 845
SexAI A'CCwGG_T
Cuts at: 0 569 2598
Size: 569 2029
SfcI C'TryA_G
Cuts at: 0 827 1495 1821 1896 2598
Size: 827 668 326 75 702
Fragments arranged by size:
827 702 668 326 75
SmlI C'TyrA_G
Cuts at: 0 788 2598
Size: 788 1810
SpeI A'CTAG_T
Cuts at: 0 858 2598
Size: 858 1740
StuI AGG'CCT
Cuts at: 0 1219 2598
Size: 1219 1379
StyI C'CwwG_G
Cuts at: 0 652 2598
Size: 652 1946
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 321 1415 2215 2426 2598
Size: 321 1094 800 211 172
Fragments arranged by size:
1094 800 321 211 172
TatI w'GTAC_w
Cuts at: 0 948 1751 2598
Size: 948 803 847
Fragments arranged by size:
948 847 803
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 132 164 1156 1188 2598
Size: 132 32 992 32 1410
Fragments arranged by size:
1410 992 132 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 308 334 2598
Size: 308 26 2264
Fragments arranged by size:
2264 308 26
XmnI GAAnn'nnTTC
Cuts at: 0 125 917 1858 2287 2598
Size: 125 792 941 429 311
Fragments arranged by size:
941 792 429 311 125
Enzymes that do cut:
AcuI AfeI AflIII AleI AlfI AloI AlwNI ApaI
ApoI AseI AvaI BanI BanII BbsI BbvCI BclI
BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI Bsp1286I
BspMI BsrBI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DrdI EaeI EarI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
MmeI MscI MslI MspA1I NspI PasI PfoI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuII SacI
SacII SapI ScaI SexAI SfcI SmlI SpeI StuI
StyI TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BcgI
BciVI BfrBI BglII BmtI BplI BsaI BsaBI BsaHI
BsaWI BsiEI BsmI BsmBI BspEI BspHI BsrDI BsrFI
BsrGI BssHII BstBI BstZ17I ClaI CspCI DraI DraIII
EagI EciI EcoNI EcoRV FseI FspAI HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PspXI PsrI PvuI RsrII SalI SanDI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SspI
SwaI XbaI XcmI XhoI XmaI ZraI