Restriction map for
pF1KB8416
(Linear) (Six-base) MAPSORT of: KIBB8416.seq Check: 9722 from: 1 to: 3156
KIBB8416.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AccI GT'mk_AC
Cuts at: 0 135 1730 3156
Size: 135 1595 1426
Fragments arranged by size:
1595 1426 135
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 1820 2131 2257 2509 2837 3123 3156
Size: 377 1443 311 126 252 328 286 33
Fragments arranged by size:
1443 377 328 311 286 252 126 33
AflII C'TTAA_G
Cuts at: 0 195 1335 3156
Size: 195 1140 1821
Fragments arranged by size:
1821 1140 195
AlwNI CAG_nnn'CTG
Cuts at: 0 106 1371 1492 2704 3156
Size: 106 1265 121 1212 452
Fragments arranged by size:
1265 1212 452 121 106
ApoI r'AATT_y
Cuts at: 0 81 569 604 644 1225 1303 1377 1431
Size: 81 488 35 40 581 78 74 54
Cuts at: 1431 1596 1972 2236 2380 3156
Size: 165 376 264 144 776
Fragments arranged by size:
776 581 488 376 264 165 144 81
78 74 54 40 35
AseI AT'TA_AT
Cuts at: 0 749 3156
Size: 749 2407
BamHI G'GATC_C
Cuts at: 0 7 1845 3156
Size: 7 1838 1311
Fragments arranged by size:
1838 1311 7
BanI G'GyrC_C
Cuts at: 0 960 3156
Size: 960 2196
BbsI GAAGACnn'nnnn_
Cuts at: 0 2079 3156
Size: 2079 1077
BclI T'GATC_A
Cuts at: 0 2559 3156
Size: 2559 597
BfrBI ATG'CAT
Cuts at: 0 124 3027 3156
Size: 124 2903 129
Fragments arranged by size:
2903 129 124
BglII A'GATC_T
Cuts at: 0 438 3156
Size: 438 2718
BlpI GC'TnA_GC
Cuts at: 0 1743 3156
Size: 1743 1413
Bme1580I G_kGCm'C
Cuts at: 0 535 1786 3156
Size: 535 1251 1370
Fragments arranged by size:
1370 1251 535
BmrI ACTGGGnnnn_n'
Cuts at: 0 2085 3156
Size: 2085 1071
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 925 3071 3107 3156
Size: 736 189 2146 36 49
Fragments arranged by size:
2146 736 189 49 36
BsaI GGTCTCn'nnnn_
Cuts at: 0 424 1642 3156
Size: 424 1218 1514
Fragments arranged by size:
1514 1218 424
BsaAI yAC'GTr
Cuts at: 0 1728 3156
Size: 1728 1428
BsaBI GATnn'nnATC
Cuts at: 0 2558 3156
Size: 2558 598
BsaHI Gr'CG_yC
Cuts at: 0 43 3156
Size: 43 3113
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 898 928 3156
Size: 898 30 2228
Fragments arranged by size:
2228 898 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1331 3156
Size: 1331 1825
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 575 3156
Size: 575 2581
BsmI GAATG_Cn'
Cuts at: 0 2283 2472 3156
Size: 2283 189 684
Fragments arranged by size:
2283 684 189
Bsp1286I G_dGCh'C
Cuts at: 0 535 1786 3156
Size: 535 1251 1370
Fragments arranged by size:
1370 1251 535
BspHI T'CATG_A
Cuts at: 0 270 3144 3156
Size: 270 2874 12
Fragments arranged by size:
2874 270 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2682 3156
Size: 2682 474
BsrDI GCAATG_nn'
Cuts at: 0 170 493 3156
Size: 170 323 2663
Fragments arranged by size:
2663 323 170
BsrGI T'GTAC_A
Cuts at: 0 561 2615 3156
Size: 561 2054 541
Fragments arranged by size:
2054 561 541
BssSI C'ACGA_G
Cuts at: 0 1313 3156
Size: 1313 1843
BstXI CCAn_nnnn'nTGG
Cuts at: 0 834 3156
Size: 834 2322
BstYI r'GATC_y
Cuts at: 0 7 297 438 772 1488 1845 3066 3156
Size: 7 290 141 334 716 357 1221 90
Fragments arranged by size:
1221 716 357 334 290 141 90 7
Bsu36I CC'TnA_GG
Cuts at: 0 956 3156
Size: 956 2200
BtsI GCAGTG_nn'
Cuts at: 0 1568 2457 2540 2690 3156
Size: 1568 889 83 150 466
Fragments arranged by size:
1568 889 466 150 83
ClaI AT'CG_AT
Cuts at: 0 116 2868 3156
Size: 116 2752 288
Fragments arranged by size:
2752 288 116
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2936 2971 3156
Size: 2936 35 185
Fragments arranged by size:
2936 185 35
DraI TTT'AAA
Cuts at: 0 767 1382 2246 3156
Size: 767 615 864 910
Fragments arranged by size:
910 864 767 615
DraIII CAC_nnn'GTG
Cuts at: 0 1361 3156
Size: 1361 1795
EaeI y'GGCC_r
Cuts at: 0 20 176 3156
Size: 20 156 2980
Fragments arranged by size:
2980 156 20
EarI CTCTTCn'nnn_
Cuts at: 0 32 1091 1850 2150 2318 3156
Size: 32 1059 759 300 168 838
Fragments arranged by size:
1059 838 759 300 168 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 736 925 1820 2131 2257 2509 2837
Size: 377 359 189 895 311 126 252 328
Cuts at: 2837 3071 3107 3123 3156
Size: 234 36 16 33
Fragments arranged by size:
895 377 359 328 311 252 234 189
126 36 33 16
EcoO109I rG'GnC_Cy
Cuts at: 0 3118 3156
Size: 3118 38
EcoRI G'AATT_C
Cuts at: 0 2380 3156
Size: 2380 776
EcoRV GAT'ATC
Cuts at: 0 797 3156
Size: 797 2359
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 382 3156
Size: 382 2774
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 195 227 423 455 2317 2349 3156
Size: 195 32 196 32 1862 32 807
Fragments arranged by size:
1862 807 196 195 32 32 32
HincII GTy'rAC
Cuts at: 0 98 136 3020 3156
Size: 98 38 2884 136
Fragments arranged by size:
2884 136 98 38
HindIII A'AGCT_T
Cuts at: 0 2756 3156
Size: 2756 400
HpaI GTT'AAC
Cuts at: 0 3020 3156
Size: 3020 136
MfeI C'AATT_G
Cuts at: 0 2792 3156
Size: 2792 364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1014 1535 1663 2502 3156
Size: 1014 521 128 839 654
Fragments arranged by size:
1014 839 654 521 128
MscI TGG'CCA
Cuts at: 0 178 3156
Size: 178 2978
MslI CAynn'nnrTG
Cuts at: 0 163 982 3156
Size: 163 819 2174
Fragments arranged by size:
2174 819 163
MspA1I CmG'CkG
Cuts at: 0 1894 2590 2632 3156
Size: 1894 696 42 524
Fragments arranged by size:
1894 696 524 42
NsiI A_TGCA'T
Cuts at: 0 126 3029 3156
Size: 126 2903 127
Fragments arranged by size:
2903 127 126
PflMI CCAn_nnn'nTGG
Cuts at: 0 1033 1916 3156
Size: 1033 883 1240
Fragments arranged by size:
1240 1033 883
PpuMI rG'GwC_Cy
Cuts at: 0 3118 3156
Size: 3118 38
PsiI TTA'TAA
Cuts at: 0 2056 3156
Size: 2056 1100
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 830 862 1895 1927 3156
Size: 830 32 1033 32 1229
Fragments arranged by size:
1229 1033 830 32 32
PstI C_TGCA'G
Cuts at: 0 1176 1998 3156
Size: 1176 822 1158
Fragments arranged by size:
1176 1158 822
PvuII CAG'CTG
Cuts at: 0 1894 2590 2632 3156
Size: 1894 696 42 524
Fragments arranged by size:
1894 696 524 42
SalI G'TCGA_C
Cuts at: 0 134 3156
Size: 134 3022
SapI GCTCTTCn'nnn_
Cuts at: 0 2150 3156
Size: 2150 1006
SfcI C'TryA_G
Cuts at: 0 1172 1994 2297 2678 2696 3156
Size: 1172 822 303 381 18 460
Fragments arranged by size:
1172 822 460 381 303 18
SmlI C'TyrA_G
Cuts at: 0 195 1335 3156
Size: 195 1140 1821
Fragments arranged by size:
1821 1140 195
SnaBI TAC'GTA
Cuts at: 0 1728 3156
Size: 1728 1428
SspI AAT'ATT
Cuts at: 0 2128 2355 3156
Size: 2128 227 801
Fragments arranged by size:
2128 801 227
StuI AGG'CCT
Cuts at: 0 1758 3156
Size: 1758 1398
StyI C'CwwG_G
Cuts at: 0 992 1091 1149 2717 3156
Size: 992 99 58 1568 439
Fragments arranged by size:
1568 992 439 99 58
SwaI ATTT'AAAT
Cuts at: 0 2246 3156
Size: 2246 910
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 196 3156
Size: 196 2960
TatI w'GTAC_w
Cuts at: 0 363 561 1873 2615 3156
Size: 363 198 1312 742 541
Fragments arranged by size:
1312 742 541 363 198
Tth111I GACn'n_nGTC
Cuts at: 0 1824 3156
Size: 1824 1332
XmnI GAAnn'nnTTC
Cuts at: 0 1227 1304 3156
Size: 1227 77 1852
Fragments arranged by size:
1852 1227 77
Enzymes that do cut:
AccI AcuI AflII AlwNI ApoI AseI BamHI BanI
BbsI BclI BfrBI BglII BlpI Bme1580I BmrI BpmI
BsaI BsaAI BsaBI BsaHI BsaXI BseRI BsgI BsmI
Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstXI BstYI
Bsu36I BtsI ClaI CspCI DraI DraIII EaeI EarI
Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I HincII HindIII
HpaI MfeI MmeI MscI MslI MspA1I NsiI PflMI
PpuMI PsiI PsrI PstI PvuII SalI SapI SfcI
SmlI SnaBI SspI StuI StyI SwaI TaqII TatI
Tth111I XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BanII BbeI BbvCI BcgI BciVI BglI
BmgBI BmtI BplI Bpu10I BpuEI BsaWI BseYI BsiEI
BsiHKAI BsiWI BsmBI BspEI BsrBI BsrFI BssHII BstAPI
BstBI BstEII BstZ17I BtgI BtgZI DrdI EagI EciI
EcoICRI EcoNI FseI FspI FspAI HaeII KasI KpnI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PspOMI PspXI PvuI RsrII SacI SacII
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SpeI SphI SrfI TstI XbaI XcmI XhoI
XmaI ZraI