Restriction map for
pF1KB8418
(Linear) (Six-base) MAPSORT of: KIBB8418.seq Check: 755 from: 1 to: 2427
KIBB8418.orf
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2396 2427
Size: 2396 31
AflIII A'CryG_T
Cuts at: 0 691 1027 2427
Size: 691 336 1400
Fragments arranged by size:
1400 691 336
ApaLI G'TGCA_C
Cuts at: 0 376 2427
Size: 376 2051
ApoI r'AATT_y
Cuts at: 0 34 78 469 477 597 850 917 1001
Size: 34 44 391 8 120 253 67 84
Cuts at: 1001 1102 1169 1337 1421 1505 1757 1774 1841
Size: 101 67 168 84 84 252 17 67
Cuts at: 1841 2177 2261 2345 2427
Size: 336 84 84 82
Fragments arranged by size:
391 336 253 252 168 120 101 84
84 84 84 84 82 67 67 67
44 34 17 8
AseI AT'TA_AT
Cuts at: 0 668 2427
Size: 668 1759
BbsI GAAGACnn'nnnn_
Cuts at: 0 114 2427
Size: 114 2313
BclI T'GATC_A
Cuts at: 0 155 2427
Size: 155 2272
Bme1580I G_kGCm'C
Cuts at: 0 380 2427
Size: 380 2047
BmrI ACTGGGnnnn_n'
Cuts at: 0 220 2427
Size: 220 2207
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2380 2427
Size: 2380 47
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 187 695 863 947 1031 1283 1367
Size: 64 123 508 168 84 84 252 84
Cuts at: 1367 1619 1703 1787 1871 2039 2123 2207 2375
Size: 252 84 84 84 168 84 84 168
Cuts at: 2375 2427
Size: 52
Fragments arranged by size:
508 252 252 168 168 168 123 84
84 84 84 84 84 84 84 64
52
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 213 398 1951 2427
Size: 61 152 185 1553 476
Fragments arranged by size:
1553 476 185 152 61
BsiHKAI G_wGCw'C
Cuts at: 0 380 2427
Size: 380 2047
Bsp1286I G_dGCh'C
Cuts at: 0 380 2427
Size: 380 2047
BsrDI GCAATG_nn'
Cuts at: 0 61 2427
Size: 61 2366
BsrFI r'CCGG_y
Cuts at: 0 1139 1475 2427
Size: 1139 336 952
Fragments arranged by size:
1139 952 336
BsrGI T'GTAC_A
Cuts at: 0 333 1114 2427
Size: 333 781 1313
Fragments arranged by size:
1313 781 333
BstYI r'GATC_y
Cuts at: 0 279 2427
Size: 279 2148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 51 2427
Size: 51 2376
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 516 551 2427
Size: 516 35 1876
Fragments arranged by size:
1876 516 35
DraI TTT'AAA
Cuts at: 0 341 2061 2427
Size: 341 1720 366
Fragments arranged by size:
1720 366 341
EaeI y'GGCC_r
Cuts at: 0 26 259 2427
Size: 26 233 2168
Fragments arranged by size:
2168 233 26
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 187 695 863 947 1031 1283 1367
Size: 64 123 508 168 84 84 252 84
Cuts at: 1367 1619 1703 1787 1871 2039 2123 2207 2375
Size: 252 84 84 84 168 84 84 168
Cuts at: 2375 2396 2427
Size: 21 31
Fragments arranged by size:
508 252 252 168 168 168 123 84
84 84 84 84 84 84 84 64
31 21
EcoRI G'AATT_C
Cuts at: 0 34 1001 1337 1421 2261 2427
Size: 34 967 336 84 840 166
Fragments arranged by size:
967 840 336 166 84 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 147 179 752 784 2388 2420 2427
Size: 147 32 573 32 1604 32 7
Fragments arranged by size:
1604 573 147 32 32 32 7
HincII GTy'rAC
Cuts at: 0 1253 2427
Size: 1253 1174
HindIII A'AGCT_T
Cuts at: 0 626 794 878 1130 1214 1234 1466 1550
Size: 626 168 84 252 84 20 232 84
Cuts at: 1550 1570 1634 1718 1802 1886 2054 2138 2306
Size: 20 64 84 84 84 168 84 168
Cuts at: 2306 2427
Size: 121
Fragments arranged by size:
626 252 232 168 168 168 121 84
84 84 84 84 84 84 64 20
20
HpaI GTT'AAC
Cuts at: 0 1253 2427
Size: 1253 1174
MscI TGG'CCA
Cuts at: 0 28 261 2427
Size: 28 233 2166
Fragments arranged by size:
2166 233 28
MslI CAynn'nnrTG
Cuts at: 0 329 356 2427
Size: 329 27 2071
Fragments arranged by size:
2071 329 27
NspI r_CATG'y
Cuts at: 0 555 695 1031 2427
Size: 555 140 336 1396
Fragments arranged by size:
1396 555 336 140
PciI A'CATG_T
Cuts at: 0 691 1027 2427
Size: 691 336 1400
Fragments arranged by size:
1400 691 336
PfoI T'CCnGG_A
Cuts at: 0 924 2427
Size: 924 1503
PsiI TTA'TAA
Cuts at: 0 392 2427
Size: 392 2035
PstI C_TGCA'G
Cuts at: 0 1098 1434 2427
Size: 1098 336 993
Fragments arranged by size:
1098 993 336
SexAI A'CCwGG_T
Cuts at: 0 116 2427
Size: 116 2311
SfcI C'TryA_G
Cuts at: 0 108 1094 1430 2427
Size: 108 986 336 997
Fragments arranged by size:
997 986 336 108
SphI G_CATG'C
Cuts at: 0 555 2427
Size: 555 1872
SspI AAT'ATT
Cuts at: 0 434 2320 2427
Size: 434 1886 107
Fragments arranged by size:
1886 434 107
StyI C'CwwG_G
Cuts at: 0 184 2427
Size: 184 2243
TatI w'GTAC_w
Cuts at: 0 333 1114 2427
Size: 333 781 1313
Fragments arranged by size:
1313 781 333
Enzymes that do cut:
AcuI AflIII ApaLI ApoI AseI BbsI BclI Bme1580I
BmrI BplI BpmI BseRI BsiHKAI Bsp1286I BsrDI BsrFI
BsrGI BstYI BtgZI CspCI DraI EaeI Eco57MI EcoRI
Hin4I HincII HindIII HpaI MscI MslI NspI PciI
PfoI PsiI PstI SexAI SfcI SphI SspI StyI
TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI AscI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmtI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtsI ClaI DraIII DrdI EagI
EarI EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspI FspAI HaeII KasI KpnI MfeI MluI MmeI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI