Restriction map for
pF1KB8419
(Linear) (Six-base) MAPSORT of: KIBB8419.seq Check: 2055 from: 1 to: 1587
KIBB8419.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AccI GT'mk_AC
Cuts at: 0 383 1587
Size: 383 1204
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 405 573 741 1329 1413 1587
Size: 137 268 168 168 588 84 174
Fragments arranged by size:
588 268 174 168 168 137 84
AleI CACnn'nnGTG
Cuts at: 0 884 1279 1587
Size: 884 395 308
Fragments arranged by size:
884 395 308
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 215 1587
Size: 215 1372
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1518 1550 1587
Size: 1518 32 37
Fragments arranged by size:
1518 37 32
ApoI r'AATT_y
Cuts at: 0 288 369 1005 1463 1575 1587
Size: 288 81 636 458 112 12
Fragments arranged by size:
636 458 288 112 81 12
AvaI C'yCGr_G
Cuts at: 0 655 1587
Size: 655 932
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1392 1425 1587
Size: 1392 33 162
Fragments arranged by size:
1392 162 33
BanI G'GyrC_C
Cuts at: 0 1370 1587
Size: 1370 217
Bme1580I G_kGCm'C
Cuts at: 0 823 1587
Size: 823 764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 821 1587
Size: 821 766
Bpu10I CC'TnA_GC
Cuts at: 0 1183 1587
Size: 1183 404
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 1587
Size: 928 659
BsaI GGTCTCn'nnnn_
Cuts at: 0 30 1587
Size: 30 1557
BsaAI yAC'GTr
Cuts at: 0 1470 1587
Size: 1470 117
BsaWI w'CCGG_w
Cuts at: 0 1373 1587
Size: 1373 214
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 319 1587
Size: 319 1268
BsmI GAATG_Cn'
Cuts at: 0 545 1126 1587
Size: 545 581 461
Fragments arranged by size:
581 545 461
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1575 1587
Size: 1575 12
Bsp1286I G_dGCh'C
Cuts at: 0 823 1587
Size: 823 764
BspHI T'CATG_A
Cuts at: 0 246 1512 1587
Size: 246 1266 75
Fragments arranged by size:
1266 246 75
BsrDI GCAATG_nn'
Cuts at: 0 1113 1587
Size: 1113 474
BstXI CCAn_nnnn'nTGG
Cuts at: 0 221 1587
Size: 221 1366
EarI CTCTTCn'nnn_
Cuts at: 0 661 829 1165 1563 1587
Size: 661 168 336 398 24
Fragments arranged by size:
661 398 336 168 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 405 573 741 821 1329 1413 1587
Size: 137 268 168 168 80 508 84 174
Fragments arranged by size:
508 268 174 168 168 137 84 80
EcoNI CCTnn'n_nnAGG
Cuts at: 0 74 1587
Size: 74 1513
EcoO109I rG'GnC_Cy
Cuts at: 0 455 1379 1587
Size: 455 924 208
Fragments arranged by size:
924 455 208
EcoRI G'AATT_C
Cuts at: 0 369 1587
Size: 369 1218
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 228 256 260 288 516 548 828 860
Size: 228 28 4 28 228 32 280 32
Cuts at: 860 1587
Size: 727
Fragments arranged by size:
727 280 228 228 32 32 28 28
4
HindIII A'AGCT_T
Cuts at: 0 417 585 921 1341 1587
Size: 417 168 336 420 246
Fragments arranged by size:
420 417 336 246 168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 1587
Size: 391 1196
MslI CAynn'nnrTG
Cuts at: 0 197 219 350 716 732 767 884 1152
Size: 197 22 131 366 16 35 117 268
Cuts at: 1152 1279 1587
Size: 127 308
Fragments arranged by size:
366 308 268 197 131 127 117 35
22 16
MspA1I CmG'CkG
Cuts at: 0 873 1415 1587
Size: 873 542 172
Fragments arranged by size:
873 542 172
NdeI CA'TA_TG
Cuts at: 0 729 1587
Size: 729 858
PflMI CCAn_nnn'nTGG
Cuts at: 0 997 1587
Size: 997 590
PpuMI rG'GwC_Cy
Cuts at: 0 455 1379 1587
Size: 455 924 208
Fragments arranged by size:
924 455 208
PsiI TTA'TAA
Cuts at: 0 188 479 815 1319 1587
Size: 188 291 336 504 268
Fragments arranged by size:
504 336 291 268 188
PvuII CAG'CTG
Cuts at: 0 1415 1587
Size: 1415 172
SmlI C'TyrA_G
Cuts at: 0 907 1587
Size: 907 680
XmnI GAAnn'nnTTC
Cuts at: 0 107 418 586 670 1258 1426 1587
Size: 107 311 168 84 588 168 161
Fragments arranged by size:
588 311 168 168 161 107 84
Enzymes that do cut:
AccI AcuI AleI AlfI AloI ApoI AvaI BaeI
BanI Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI BsaWI
BseRI BsmI BsmBI Bsp1286I BspHI BsrDI BstXI EarI
Eco57MI EcoNI EcoO109I EcoRI Hin4I HindIII MmeI MslI
MspA1I NdeI PflMI PpuMI PsiI PvuII SmlI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BamHI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
BsaBI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI
BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI