Restriction map for
pF1KB8425
(Linear) (Six-base) MAPSORT of: KIBB8425.seq Check: 6892 from: 1 to: 2064
KIBB8425.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 533 717 1138 1685 2036 2064
Size: 95 438 184 421 547 351 28
Fragments arranged by size:
547 438 421 351 184 95 28
AfeI AGC'GCT
Cuts at: 0 704 2064
Size: 704 1360
AflII C'TTAA_G
Cuts at: 0 1777 2064
Size: 1777 287
AhdI GACnn_n'nnGTC
Cuts at: 0 179 2064
Size: 179 1885
AlwNI CAG_nnn'CTG
Cuts at: 0 261 913 2064
Size: 261 652 1151
Fragments arranged by size:
1151 652 261
ApaLI G'TGCA_C
Cuts at: 0 1515 2064
Size: 1515 549
ApoI r'AATT_y
Cuts at: 0 103 529 759 805 814 1336 1810 1981
Size: 103 426 230 46 9 522 474 171
Cuts at: 1981 2064
Size: 83
Fragments arranged by size:
522 474 426 230 171 103 83 46
9
AvaI C'yCGr_G
Cuts at: 0 563 1849 2064
Size: 563 1286 215
Fragments arranged by size:
1286 563 215
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1565 1598 2064
Size: 1565 33 466
Fragments arranged by size:
1565 466 33
BanI G'GyrC_C
Cuts at: 0 870 2064
Size: 870 1194
BanII G_rGCy'C
Cuts at: 0 1562 2064
Size: 1562 502
BbsI GAAGACnn'nnnn_
Cuts at: 0 146 178 278 2064
Size: 146 32 100 1786
Fragments arranged by size:
1786 146 100 32
BbvCI CC'TCA_GC
Cuts at: 0 1623 2064
Size: 1623 441
BglII A'GATC_T
Cuts at: 0 1256 2064
Size: 1256 808
Bme1580I G_kGCm'C
Cuts at: 0 1519 2064
Size: 1519 545
BmrI ACTGGGnnnn_n'
Cuts at: 0 1506 1954 2055 2064
Size: 1506 448 101 9
Fragments arranged by size:
1506 448 101 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 627 1012 1452 2064
Size: 509 118 385 440 612
Fragments arranged by size:
612 509 440 385 118
Bpu10I CC'TnA_GC
Cuts at: 0 1623 2064
Size: 1623 441
BsaI GGTCTCn'nnnn_
Cuts at: 0 485 1215 2064
Size: 485 730 849
Fragments arranged by size:
849 730 485
BsaAI yAC'GTr
Cuts at: 0 1092 2064
Size: 1092 972
BsaBI GATnn'nnATC
Cuts at: 0 204 2064
Size: 204 1860
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 486 808 1256 1432 1459 2064
Size: 486 322 448 176 27 605
Fragments arranged by size:
605 486 448 322 176 27
BseYI C'CCAG_C
Cuts at: 0 304 495 1146 1561 2021 2064
Size: 304 191 651 415 460 43
Fragments arranged by size:
651 460 415 304 191 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 693 2064
Size: 693 1371
BsiHKAI G_wGCw'C
Cuts at: 0 1100 1519 2064
Size: 1100 419 545
Fragments arranged by size:
1100 545 419
BsmI GAATG_Cn'
Cuts at: 0 230 2064
Size: 230 1834
Bsp1286I G_dGCh'C
Cuts at: 0 1100 1519 1562 2064
Size: 1100 419 43 502
Fragments arranged by size:
1100 502 419 43
BspHI T'CATG_A
Cuts at: 0 342 723 1044 2064
Size: 342 381 321 1020
Fragments arranged by size:
1020 381 342 321
BsrGI T'GTAC_A
Cuts at: 0 1713 2064
Size: 1713 351
BstYI r'GATC_y
Cuts at: 0 308 1256 1845 2064
Size: 308 948 589 219
Fragments arranged by size:
948 589 308 219
Bsu36I CC'TnA_GG
Cuts at: 0 1442 2064
Size: 1442 622
BtsI GCAGTG_nn'
Cuts at: 0 1208 1268 1601 2064
Size: 1208 60 333 463
Fragments arranged by size:
1208 463 333 60
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1124 1159 2064
Size: 1124 35 905
Fragments arranged by size:
1124 905 35
DraI TTT'AAA
Cuts at: 0 23 813 2064
Size: 23 790 1251
Fragments arranged by size:
1251 790 23
EaeI y'GGCC_r
Cuts at: 0 697 2064
Size: 697 1367
EarI CTCTTCn'nnn_
Cuts at: 0 509 778 785 881 1043 1529 1661 2064
Size: 509 269 7 96 162 486 132 403
Fragments arranged by size:
509 486 403 269 162 132 96 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 509 533 627 717 1012 1138 1452
Size: 95 414 24 94 90 295 126 314
Cuts at: 1452 1685 2036 2064
Size: 233 351 28
Fragments arranged by size:
414 351 314 295 233 126 95 94
90 28 24
EcoO109I rG'GnC_Cy
Cuts at: 0 1772 2064
Size: 1772 292
EcoRI G'AATT_C
Cuts at: 0 529 805 1810 2064
Size: 529 276 1005 254
Fragments arranged by size:
1005 529 276 254
FspI TGC'GCA
Cuts at: 0 962 2064
Size: 962 1102
HaeII r_GCGC'y
Cuts at: 0 706 2064
Size: 706 1358
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 165 197 484 516 625 657 1492 1524
Size: 165 32 287 32 109 32 835 32
Cuts at: 1524 1583 1615 1939 1971 2064
Size: 59 32 324 32 93
Fragments arranged by size:
835 324 287 165 109 93 59 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1467 2064
Size: 1467 597
HindIII A'AGCT_T
Cuts at: 0 1689 2064
Size: 1689 375
MslI CAynn'nnrTG
Cuts at: 0 368 557 2064
Size: 368 189 1507
Fragments arranged by size:
1507 368 189
MspA1I CmG'CkG
Cuts at: 0 85 631 836 1116 1627 2064
Size: 85 546 205 280 511 437
Fragments arranged by size:
546 511 437 280 205 85
NspI r_CATG'y
Cuts at: 0 562 1057 2064
Size: 562 495 1007
Fragments arranged by size:
1007 562 495
PflMI CCAn_nnn'nTGG
Cuts at: 0 1594 2064
Size: 1594 470
PstI C_TGCA'G
Cuts at: 0 294 1992 2064
Size: 294 1698 72
Fragments arranged by size:
1698 294 72
PvuII CAG'CTG
Cuts at: 0 836 1116 2064
Size: 836 280 948
Fragments arranged by size:
948 836 280
SapI GCTCTTCn'nnn_
Cuts at: 0 1043 2064
Size: 1043 1021
SfcI C'TryA_G
Cuts at: 0 290 1988 2064
Size: 290 1698 76
Fragments arranged by size:
1698 290 76
SmaI CCC'GGG
Cuts at: 0 565 2064
Size: 565 1499
SmlI C'TyrA_G
Cuts at: 0 1777 2064
Size: 1777 287
SpeI A'CTAG_T
Cuts at: 0 1162 2064
Size: 1162 902
StuI AGG'CCT
Cuts at: 0 1087 2064
Size: 1087 977
StyI C'CwwG_G
Cuts at: 0 1523 1678 2064
Size: 1523 155 386
Fragments arranged by size:
1523 386 155
TatI w'GTAC_w
Cuts at: 0 87 211 252 1713 2064
Size: 87 124 41 1461 351
Fragments arranged by size:
1461 351 124 87 41
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 575 607 998 1030 1214 1246 2064
Size: 575 32 391 32 184 32 818
Fragments arranged by size:
818 575 391 184 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 829 2064
Size: 829 1235
XbaI T'CTAG_A
Cuts at: 0 1305 2064
Size: 1305 759
XmaI C'CCGG_G
Cuts at: 0 563 2064
Size: 563 1501
Enzymes that do cut:
AcuI AfeI AflII AhdI AlwNI ApaLI ApoI AvaI
BaeI BanI BanII BbsI BbvCI BglII Bme1580I BmrI
BpmI Bpu10I BsaI BsaAI BsaBI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BsrGI BstYI Bsu36I BtsI
CspCI DraI EaeI EarI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII MslI MspA1I NspI PflMI
PstI PvuII SapI SfcI SmaI SmlI SpeI StuI
StyI TatI TstI Tth111I XbaI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AleI
AlfI AloI ApaI AscI AseI AsiSI AvrII BamHI
BbeI BcgI BciVI BclI BfrBI BglI BlpI BmgBI
BmtI BplI BpuEI BsaHI BsaWI BsaXI BsiEI BsiWI
BsmBI BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV FalI
FseI FspAI HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SphI SrfI SspI
SwaI TaqII XcmI XhoI XmnI ZraI