(Linear) (Six-base) MAPSORT of: KIBB8426.seq  Check: 5628  from: 1  to: 654



KIBB8426.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      70     654 
   Size:         70     584
 
ApoI r'AATT_y

Cuts at:      0     109     145     654 
   Size:        109      36     509

  Fragments arranged by size: 

                 509     109      36
 
BanII G_rGCy'C

Cuts at:      0     267     568     654 
   Size:        267     301      86

  Fragments arranged by size: 

                 301     267      86
 
BbvCI CC'TCA_GC

Cuts at:      0     604     654 
   Size:        604      50
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     195     654 
   Size:        195     459
 
BclI T'GATC_A

Cuts at:      0     359     654 
   Size:        359     295
 
BmtI G_CTAG'C

Cuts at:      0     343     654 
   Size:        343     311
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     408     654 
   Size:        408     246
 
Bpu10I CC'TnA_GC

Cuts at:      0     567     604     638     654 
   Size:        567      37      34      16

  Fragments arranged by size: 

                 567      37      34      16
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     419     615     654 
   Size:        419     196      39

  Fragments arranged by size: 

                 419     196      39
 
BsaBI GATnn'nnATC

Cuts at:      0     395     654 
   Size:        395     259
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     259     654 
   Size:        259     395
 
BsiEI CG_ry'CG

Cuts at:      0     260     654 
   Size:        260     394
 
BsmI GAATG_Cn'

Cuts at:      0     341     654 
   Size:        341     313
 
Bsp1286I G_dGCh'C

Cuts at:      0     267     568     654 
   Size:        267     301      86

  Fragments arranged by size: 

                 301     267      86
 
BsrGI T'GTAC_A

Cuts at:      0     501     654 
   Size:        501     153
 
BssHII G'CGCG_C

Cuts at:      0     618     654 
   Size:        618      36
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      80     654 
   Size:         80     574
 
BstEII G'GTnAC_C

Cuts at:      0     608     654 
   Size:        608      46
 
BtgI C'CryG_G

Cuts at:      0     613     654 
   Size:        613      41
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     122     157     654 
   Size:        122      35     497

  Fragments arranged by size: 

                 497     122      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      15     654 
   Size:         15     639
 
EaeI y'GGCC_r

Cuts at:      0      11     257     654 
   Size:         11     246     397

  Fragments arranged by size: 

                 397     246      11
 
EagI C'GGCC_G

Cuts at:      0     257     654 
   Size:        257     397
 
EarI CTCTTCn'nnn_

Cuts at:      0     485     654 
   Size:        485     169
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     408     654 
   Size:        408     246
 
EcoO109I rG'GnC_Cy

Cuts at:      0     384     627     654 
   Size:        384     243      27

  Fragments arranged by size: 

                 384     243      27
 
EcoRV GAT'ATC

Cuts at:      0     282     654 
   Size:        282     372
 
HincII GTy'rAC

Cuts at:      0     323     654 
   Size:        323     331
 
HpaI GTT'AAC

Cuts at:      0     323     654 
   Size:        323     331
 
MspA1I CmG'CkG

Cuts at:      0     603     615     654 
   Size:        603      12      39

  Fragments arranged by size: 

                 603      39      12
 
NheI G'CTAG_C

Cuts at:      0     339     654 
   Size:        339     315
 
PpuMI rG'GwC_Cy

Cuts at:      0     384     654 
   Size:        384     270
 
PvuII CAG'CTG

Cuts at:      0     603     654 
   Size:        603      51
 
SacII CC_GC'GG

Cuts at:      0     616     654 
   Size:        616      38
 
SmlI C'TyrA_G

Cuts at:      0     434     594     654 
   Size:        434     160      60

  Fragments arranged by size: 

                 434     160      60
 
TatI w'GTAC_w

Cuts at:      0     501     654 
   Size:        501     153
 

 Enzymes that do cut:

    AlwNI     ApoI    BanII    BbvCI    BciVI     BclI     BmtI     BpmI
   Bpu10I    BpuEI    BsaBI    BseRI    BsiEI     BsmI Bsp1286I    BsrGI
   BssHII   BstAPI   BstEII     BtgI    CspCI     DrdI     EaeI     EagI
     EarI  Eco57MI EcoO109I    EcoRV   HincII     HpaI   MspA1I     NheI
    PpuMI    PvuII    SacII     SmlI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI     BcgI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI    BstBI    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI     DraI   DraIII     EciI  EcoICRI
    EcoNI    EcoRI     FalI     FseI     FspI    FspAI    HaeII    Hin4I
  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI