Restriction map for
pF1KB8428
(Linear) (Six-base) MAPSORT of: KIBB8428.seq Check: 8975 from: 1 to: 1401
KIBB8428.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 708 943 1401
Size: 708 235 458
Fragments arranged by size:
708 458 235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 1401
Size: 592 809
AflII C'TTAA_G
Cuts at: 0 637 1401
Size: 637 764
AflIII A'CryG_T
Cuts at: 0 1085 1401
Size: 1085 316
AgeI A'CCGG_T
Cuts at: 0 527 1401
Size: 527 874
AhdI GACnn_n'nnGTC
Cuts at: 0 945 1401
Size: 945 456
AleI CACnn'nnGTG
Cuts at: 0 593 1401
Size: 593 808
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1157 1401
Size: 1157 244
AlwNI CAG_nnn'CTG
Cuts at: 0 64 469 750 801 810 1032 1170 1270
Size: 64 405 281 51 9 222 138 100
Cuts at: 1270 1401
Size: 131
Fragments arranged by size:
405 281 222 138 131 100 64 51
9
ApaI G_GGCC'C
Cuts at: 0 1219 1401
Size: 1219 182
ApaLI G'TGCA_C
Cuts at: 0 1081 1401
Size: 1081 320
AvaI C'yCGr_G
Cuts at: 0 37 148 1196 1401
Size: 37 111 1048 205
Fragments arranged by size:
1048 205 111 37
BanI G'GyrC_C
Cuts at: 0 1175 1401
Size: 1175 226
BanII G_rGCy'C
Cuts at: 0 265 1060 1130 1219 1401
Size: 265 795 70 89 182
Fragments arranged by size:
795 265 182 89 70
BbsI GAAGACnn'nnnn_
Cuts at: 0 572 1401
Size: 572 829
BbvCI CC'TCA_GC
Cuts at: 0 487 1401
Size: 487 914
BciVI GTATCCnnnnn_n'
Cuts at: 0 229 1401
Size: 229 1172
BclI T'GATC_A
Cuts at: 0 812 1401
Size: 812 589
BlpI GC'TnA_GC
Cuts at: 0 599 1054 1256 1401
Size: 599 455 202 145
Fragments arranged by size:
599 455 202 145
Bme1580I G_kGCm'C
Cuts at: 0 665 1085 1219 1401
Size: 665 420 134 182
Fragments arranged by size:
665 420 182 134
BmgBI CAC'GTC
Cuts at: 0 346 1401
Size: 346 1055
BmrI ACTGGGnnnn_n'
Cuts at: 0 212 1401
Size: 212 1189
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1380 1401
Size: 1380 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1057 1350 1401
Size: 1057 293 51
Fragments arranged by size:
1057 293 51
Bpu10I CC'TnA_GC
Cuts at: 0 487 1401
Size: 487 914
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 1114 1401
Size: 709 405 287
Fragments arranged by size:
709 405 287
BsaWI w'CCGG_w
Cuts at: 0 527 704 1401
Size: 527 177 697
Fragments arranged by size:
697 527 177
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 394 409 424 439 1401
Size: 394 15 15 15 962
Fragments arranged by size:
962 394 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 370 742 745 1087 1401
Size: 370 372 3 342 314
Fragments arranged by size:
372 370 342 314 3
BseYI C'CCAG_C
Cuts at: 0 658 1401
Size: 658 743
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 418 1401
Size: 368 50 983
Fragments arranged by size:
983 368 50
BsiHKAI G_wGCw'C
Cuts at: 0 1085 1130 1401
Size: 1085 45 271
Fragments arranged by size:
1085 271 45
BsmBI CGTCTCn'nnnn_
Cuts at: 0 700 1401
Size: 700 701
Bsp1286I G_dGCh'C
Cuts at: 0 265 665 1060 1085 1130 1219 1401
Size: 265 400 395 25 45 89 182
Fragments arranged by size:
400 395 265 182 89 45 25
BspHI T'CATG_A
Cuts at: 0 1182 1401
Size: 1182 219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 512 708 746 809 943 1401
Size: 512 196 38 63 134 458
Fragments arranged by size:
512 458 196 134 63 38
BsrBI CCG'CTC
Cuts at: 0 579 780 1401
Size: 579 201 621
Fragments arranged by size:
621 579 201
BsrDI GCAATG_nn'
Cuts at: 0 332 1401
Size: 332 1069
BsrFI r'CCGG_y
Cuts at: 0 527 903 1260 1401
Size: 527 376 357 141
Fragments arranged by size:
527 376 357 141
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1170 1401
Size: 1170 231
BtsI GCAGTG_nn'
Cuts at: 0 208 830 1271 1401
Size: 208 622 441 130
Fragments arranged by size:
622 441 208 130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 977 1012 1401
Size: 977 35 389
Fragments arranged by size:
977 389 35
EaeI y'GGCC_r
Cuts at: 0 228 1401
Size: 228 1173
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 690 1401
Size: 690 711
EcoICRI GAG'CTC
Cuts at: 0 1128 1401
Size: 1128 273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 1057 1350 1401
Size: 592 465 293 51
Fragments arranged by size:
592 465 293 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 378 742 1401
Size: 378 364 659
Fragments arranged by size:
659 378 364
EcoO109I rG'GnC_Cy
Cuts at: 0 798 861 1017 1215 1216 1401
Size: 798 63 156 198 1 185
Fragments arranged by size:
798 198 185 156 63 1
FspI TGC'GCA
Cuts at: 0 754 1401
Size: 754 647
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 46 78 931 963 1401
Size: 46 32 853 32 438
Fragments arranged by size:
853 438 46 32 32
HindIII A'AGCT_T
Cuts at: 0 601 1401
Size: 601 800
MscI TGG'CCA
Cuts at: 0 230 1401
Size: 230 1171
MslI CAynn'nnrTG
Cuts at: 0 446 593 1181 1312 1401
Size: 446 147 588 131 89
Fragments arranged by size:
588 446 147 131 89
MspA1I CmG'CkG
Cuts at: 0 352 820 843 1401
Size: 352 468 23 558
Fragments arranged by size:
558 468 352 23
NaeI GCC'GGC
Cuts at: 0 905 1262 1401
Size: 905 357 139
Fragments arranged by size:
905 357 139
NgoMIV G'CCGG_C
Cuts at: 0 903 1260 1401
Size: 903 357 141
Fragments arranged by size:
903 357 141
NspI r_CATG'y
Cuts at: 0 159 1089 1401
Size: 159 930 312
Fragments arranged by size:
930 312 159
PciI A'CATG_T
Cuts at: 0 1085 1401
Size: 1085 316
PpuMI rG'GwC_Cy
Cuts at: 0 798 1401
Size: 798 603
PshAI GACnn'nnGTC
Cuts at: 0 1356 1401
Size: 1356 45
PspOMI G'GGCC_C
Cuts at: 0 1215 1401
Size: 1215 186
PstI C_TGCA'G
Cuts at: 0 806 837 924 954 1166 1401
Size: 806 31 87 30 212 235
Fragments arranged by size:
806 235 212 87 31 30
SacI G_AGCT'C
Cuts at: 0 1130 1401
Size: 1130 271
SexAI A'CCwGG_T
Cuts at: 0 1109 1401
Size: 1109 292
SfcI C'TryA_G
Cuts at: 0 742 802 833 920 950 1162 1401
Size: 742 60 31 87 30 212 239
Fragments arranged by size:
742 239 212 87 60 31 30
SmaI CCC'GGG
Cuts at: 0 1198 1401
Size: 1198 203
SmlI C'TyrA_G
Cuts at: 0 637 688 1129 1401
Size: 637 51 441 272
Fragments arranged by size:
637 441 272 51
SphI G_CATG'C
Cuts at: 0 159 1401
Size: 159 1242
SrfI GCCC'GGGC
Cuts at: 0 1198 1401
Size: 1198 203
StuI AGG'CCT
Cuts at: 0 1138 1401
Size: 1138 263
StyI C'CwwG_G
Cuts at: 0 280 1295 1401
Size: 280 1015 106
Fragments arranged by size:
1015 280 106
TatI w'GTAC_w
Cuts at: 0 410 1401
Size: 410 991
Tth111I GACn'n_nGTC
Cuts at: 0 1283 1401
Size: 1283 118
XbaI T'CTAG_A
Cuts at: 0 30 1401
Size: 30 1371
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1311 1401
Size: 1311 90
XmaI C'CCGG_G
Cuts at: 0 1196 1401
Size: 1196 205
Enzymes that do cut:
AarI AcuI AflII AflIII AgeI AhdI AleI AlfI
AlwNI ApaI ApaLI AvaI BanI BanII BbsI BbvCI
BciVI BclI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI
BtsI CspCI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I
FspI Hin4I HindIII MscI MslI MspA1I NaeI NgoMIV
NspI PciI PpuMI PshAI PspOMI PstI SacI SexAI
SfcI SmaI SmlI SphI SrfI StuI StyI TatI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AloI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BcgI BfrBI
BglI BglII BmtI BsaI BsaAI BsaBI BsaHI BsiEI
BsiWI BsmI BspEI BsrGI BssHII BssSI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI DraI
DraIII DrdI EagI EarI EcoRI EcoRV FalI FseI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MmeI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SspI SwaI TaqII TstI XhoI XmnI ZraI