Restriction map for
pF1KB8429
(Linear) (Six-base) MAPSORT of: KIBB8429.seq Check: 8172 from: 1 to: 1305
KIBB8429.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1026 1305
Size: 1026 279
AatII G_ACGT'C
Cuts at: 0 971 1305
Size: 971 334
AccI GT'mk_AC
Cuts at: 0 871 1305
Size: 871 434
Acc65I G'GTAC_C
Cuts at: 0 1194 1305
Size: 1194 111
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 429 613 709 1305
Size: 214 215 184 96 596
Fragments arranged by size:
596 215 214 184 96
AfeI AGC'GCT
Cuts at: 0 1291 1305
Size: 1291 14
AflIII A'CryG_T
Cuts at: 0 767 1305
Size: 767 538
AgeI A'CCGG_T
Cuts at: 0 132 1224 1305
Size: 132 1092 81
Fragments arranged by size:
1092 132 81
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 280 1305
Size: 280 1025
AlwNI CAG_nnn'CTG
Cuts at: 0 954 1011 1146 1149 1305
Size: 954 57 135 3 156
Fragments arranged by size:
954 156 135 57 3
ApaI G_GGCC'C
Cuts at: 0 777 1305
Size: 777 528
ApaLI G'TGCA_C
Cuts at: 0 319 580 1305
Size: 319 261 725
Fragments arranged by size:
725 319 261
AvaI C'yCGr_G
Cuts at: 0 655 1305
Size: 655 650
BanI G'GyrC_C
Cuts at: 0 135 1194 1305
Size: 135 1059 111
Fragments arranged by size:
1059 135 111
BanII G_rGCy'C
Cuts at: 0 709 777 845 1171 1305
Size: 709 68 68 326 134
Fragments arranged by size:
709 326 134 68 68
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 396 430 1305
Size: 396 34 875
Fragments arranged by size:
875 396 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1282 1305
Size: 1282 23
BlpI GC'TnA_GC
Cuts at: 0 505 733 1305
Size: 505 228 572
Fragments arranged by size:
572 505 228
Bme1580I G_kGCm'C
Cuts at: 0 323 584 777 1305
Size: 323 261 193 528
Fragments arranged by size:
528 323 261 193
BmgBI CAC'GTC
Cuts at: 0 540 729 1305
Size: 540 189 576
Fragments arranged by size:
576 540 189
BmrI ACTGGGnnnn_n'
Cuts at: 0 755 1107 1305
Size: 755 352 198
Fragments arranged by size:
755 352 198
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1125 1185 1305
Size: 1125 60 120
Fragments arranged by size:
1125 120 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 994 1305
Size: 229 765 311
Fragments arranged by size:
765 311 229
BsaI GGTCTCn'nnnn_
Cuts at: 0 581 1305
Size: 581 724
BsaBI GATnn'nnATC
Cuts at: 0 637 1305
Size: 637 668
BsaHI Gr'CG_yC
Cuts at: 0 968 1181 1305
Size: 968 213 124
Fragments arranged by size:
968 213 124
BsaWI w'CCGG_w
Cuts at: 0 132 920 1224 1305
Size: 132 788 304 81
Fragments arranged by size:
788 304 132 81
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 38 226 1117 1174 1305
Size: 38 188 891 57 131
Fragments arranged by size:
891 188 131 57 38
BseYI C'CCAG_C
Cuts at: 0 28 597 944 1190 1236 1305
Size: 28 569 347 246 46 69
Fragments arranged by size:
569 347 246 69 46 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 253 283 547 1305
Size: 169 84 30 264 758
Fragments arranged by size:
758 264 169 84 30
BsiEI CG_ry'CG
Cuts at: 0 741 745 1305
Size: 741 4 560
Fragments arranged by size:
741 560 4
BsiHKAI G_wGCw'C
Cuts at: 0 323 438 584 614 734 791 845 1111
Size: 323 115 146 30 120 57 54 266
Cuts at: 1111 1171 1305
Size: 60 134
Fragments arranged by size:
323 266 146 134 120 115 60 57
54 30
Bsp1286I G_dGCh'C
Cuts at: 0 323 438 584 614 709 734 777 791
Size: 323 115 146 30 95 25 43 14
Cuts at: 791 845 1111 1171 1305
Size: 54 266 60 134
Fragments arranged by size:
323 266 146 134 115 95 60 54
43 30 25 14
BspEI T'CCGG_A
Cuts at: 0 920 1305
Size: 920 385
BspHI T'CATG_A
Cuts at: 0 438 1305
Size: 438 867
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 482 692 1026 1304 1305
Size: 482 210 334 278 1
Fragments arranged by size:
482 334 278 210 1
BsrBI CCG'CTC
Cuts at: 0 249 1305
Size: 249 1056
BsrDI GCAATG_nn'
Cuts at: 0 992 1074 1305
Size: 992 82 231
Fragments arranged by size:
992 231 82
BsrFI r'CCGG_y
Cuts at: 0 132 740 1224 1305
Size: 132 608 484 81
Fragments arranged by size:
608 484 132 81
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1149 1305
Size: 1149 156
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 384 1305
Size: 57 327 921
Fragments arranged by size:
921 327 57
BstYI r'GATC_y
Cuts at: 0 197 1305
Size: 197 1108
BstZ17I GTA'TAC
Cuts at: 0 872 1305
Size: 872 433
Bsu36I CC'TnA_GG
Cuts at: 0 881 1305
Size: 881 424
BtgI C'CryG_G
Cuts at: 0 327 750 1305
Size: 327 423 555
Fragments arranged by size:
555 423 327
BtsI GCAGTG_nn'
Cuts at: 0 157 283 1305
Size: 157 126 1022
Fragments arranged by size:
1022 157 126
DraIII CAC_nnn'GTG
Cuts at: 0 911 1305
Size: 911 394
EaeI y'GGCC_r
Cuts at: 0 41 738 742 807 956 1154 1274 1305
Size: 41 697 4 65 149 198 120 31
Fragments arranged by size:
697 198 149 120 65 41 31 4
EagI C'GGCC_G
Cuts at: 0 738 742 1305
Size: 738 4 563
Fragments arranged by size:
738 563 4
EarI CTCTTCn'nnn_
Cuts at: 0 239 340 493 796 850 1159 1305
Size: 239 101 153 303 54 309 146
Fragments arranged by size:
309 303 239 153 146 101 54
EcoICRI GAG'CTC
Cuts at: 0 843 1169 1305
Size: 843 326 136
Fragments arranged by size:
843 326 136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 229 429 613 709 994 1305
Size: 214 15 200 184 96 285 311
Fragments arranged by size:
311 285 214 200 184 96 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 337 688 1305
Size: 337 351 617
Fragments arranged by size:
617 351 337
EcoO109I rG'GnC_Cy
Cuts at: 0 558 681 774 978 1305
Size: 558 123 93 204 327
Fragments arranged by size:
558 327 204 123 93
FseI GG_CCGG'CC
Cuts at: 0 744 1305
Size: 744 561
HaeII r_GCGC'y
Cuts at: 0 954 1293 1305
Size: 954 339 12
Fragments arranged by size:
954 339 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 89 121 358 390 612 644 1305
Size: 89 32 237 32 222 32 661
Fragments arranged by size:
661 237 222 89 32 32 32
KpnI G_GTAC'C
Cuts at: 0 1198 1305
Size: 1198 107
MscI TGG'CCA
Cuts at: 0 809 1305
Size: 809 496
MspA1I CmG'CkG
Cuts at: 0 192 460 737 1146 1305
Size: 192 268 277 409 159
Fragments arranged by size:
409 277 268 192 159
NaeI GCC'GGC
Cuts at: 0 742 1305
Size: 742 563
NgoMIV G'CCGG_C
Cuts at: 0 740 1305
Size: 740 565
NspI r_CATG'y
Cuts at: 0 771 1137 1305
Size: 771 366 168
Fragments arranged by size:
771 366 168
PasI CC'CwG_GG
Cuts at: 0 777 1305
Size: 777 528
PciI A'CATG_T
Cuts at: 0 767 1305
Size: 767 538
PfoI T'CCnGG_A
Cuts at: 0 338 419 971 1305
Size: 338 81 552 334
Fragments arranged by size:
552 338 334 81
PpuMI rG'GwC_Cy
Cuts at: 0 681 1305
Size: 681 624
PspOMI G'GGCC_C
Cuts at: 0 773 1305
Size: 773 532
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 796 828 1305
Size: 796 32 477
Fragments arranged by size:
796 477 32
PstI C_TGCA'G
Cuts at: 0 479 1305
Size: 479 826
PvuII CAG'CTG
Cuts at: 0 192 1146 1305
Size: 192 954 159
Fragments arranged by size:
954 192 159
SacI G_AGCT'C
Cuts at: 0 845 1171 1305
Size: 845 326 134
Fragments arranged by size:
845 326 134
SapI GCTCTTCn'nnn_
Cuts at: 0 239 796 850 1159 1305
Size: 239 557 54 309 146
Fragments arranged by size:
557 309 239 146 54
ScaI AGT'ACT
Cuts at: 0 280 1305
Size: 280 1025
SfcI C'TryA_G
Cuts at: 0 475 1305
Size: 475 830
SgrAI Cr'CCGG_yG
Cuts at: 0 132 1224 1305
Size: 132 1092 81
Fragments arranged by size:
1092 132 81
StyI C'CwwG_G
Cuts at: 0 50 108 225 1265 1305
Size: 50 58 117 1040 40
Fragments arranged by size:
1040 117 58 50 40
TatI w'GTAC_w
Cuts at: 0 278 1305
Size: 278 1027
Tth111I GACn'n_nGTC
Cuts at: 0 541 1305
Size: 541 764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1263 1305
Size: 1263 42
ZraI GAC'GTC
Cuts at: 0 969 1305
Size: 969 336
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AgeI
AlfI AlwNI ApaI ApaLI AvaI BanI BanII BcgI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI BsaI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BstAPI
BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraIII EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII
Hin4I KpnI MscI MspA1I NaeI NgoMIV NspI PasI
PciI PfoI PpuMI PspOMI PsrI PstI PvuII SacI
SapI ScaI SfcI SgrAI StyI TatI Tth111I XcmI
ZraI
Enzymes that do not cut:
AclI AflII AhdI AleI AloI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BmtI Bpu10I BpuEI BsaAI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BssSI BstBI BstEII
BtgZI ClaI CspCI DraI DrdI EciI EcoRI EcoRV
FalI FspI FspAI HincII HindIII HpaI KasI MfeI
MluI MmeI MslI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PvuI RsrII SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TstI XbaI
XhoI XmaI XmnI