(Linear) (Six-base) MAPSORT of: KIBB8430.seq  Check: 5701  from: 1  to: 771



KIBB8430.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     771 
   Size:        268     503
 
ApoI r'AATT_y

Cuts at:      0     297     771 
   Size:        297     474
 
BanII G_rGCy'C

Cuts at:      0     425     771 
   Size:        425     346
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      80     771 
   Size:         80     691
 
BglII A'GATC_T

Cuts at:      0     218     498     771 
   Size:        218     280     273

  Fragments arranged by size: 

                 280     273     218
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     590     605     771 
   Size:        340     250      15     166

  Fragments arranged by size: 

                 340     250     166      15
 
BsiEI CG_ry'CG

Cuts at:      0      17     771 
   Size:         17     754
 
Bsp1286I G_dGCh'C

Cuts at:      0     425     771 
   Size:        425     346
 
BstYI r'GATC_y

Cuts at:      0     218     498     771 
   Size:        218     280     273

  Fragments arranged by size: 

                 280     273     218
 
BtsI GCAGTG_nn'

Cuts at:      0     299     662     771 
   Size:        299     363     109

  Fragments arranged by size: 

                 363     299     109
 
EarI CTCTTCn'nnn_

Cuts at:      0     162     771 
   Size:        162     609
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     340     590     605     771 
   Size:        268      72     250      15     166

  Fragments arranged by size: 

                 268     250     166      72      15
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     668     700     771 
   Size:        668      32      71

  Fragments arranged by size: 

                 668      71      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     771 
   Size:        124     647
 
MslI CAynn'nnrTG

Cuts at:      0     358     771 
   Size:        358     413
 
MspA1I CmG'CkG

Cuts at:      0     700     771 
   Size:        700      71
 
PasI CC'CwG_GG

Cuts at:      0     373     771 
   Size:        373     398
 
PvuII CAG'CTG

Cuts at:      0     700     771 
   Size:        700      71
 
SfcI C'TryA_G

Cuts at:      0     492     771 
   Size:        492     279
 
SspI AAT'ATT

Cuts at:      0     284     771 
   Size:        284     487
 
StyI C'CwwG_G

Cuts at:      0     147     771 
   Size:        147     624
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     582     614     771 
   Size:        582      32     157

  Fragments arranged by size: 

                 582     157      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     156     629     771 
   Size:        156     473     142

  Fragments arranged by size: 

                 473     156     142
 

 Enzymes that do cut:

     AcuI     ApoI    BanII     BbsI    BglII     BpmI    BsiEI Bsp1286I
    BstYI     BtsI     EarI  Eco57MI    Hin4I     MmeI     MslI   MspA1I
     PasI    PvuII     SfcI     SspI     StyI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI