Restriction map for
pF1KB8431
(Linear) (Six-base) MAPSORT of: KIBB8431.seq Check: 9607 from: 1 to: 1887
KIBB8431.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AccI GT'mk_AC
Cuts at: 0 1216 1887
Size: 1216 671
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 110 175 1887
Size: 38 72 65 1712
Fragments arranged by size:
1712 72 65 38
AflIII A'CryG_T
Cuts at: 0 1824 1887
Size: 1824 63
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1548 1887
Size: 1548 339
ApoI r'AATT_y
Cuts at: 0 76 261 385 441 469 792 1088 1207
Size: 76 185 124 56 28 323 296 119
Cuts at: 1207 1350 1887
Size: 143 537
Fragments arranged by size:
537 323 296 185 143 124 119 76
56 28
AseI AT'TA_AT
Cuts at: 0 227 1887
Size: 227 1660
AvaI C'yCGr_G
Cuts at: 0 416 1887
Size: 416 1471
BbsI GAAGACnn'nnnn_
Cuts at: 0 129 1255 1887
Size: 129 1126 632
Fragments arranged by size:
1126 632 129
BbvCI CC'TCA_GC
Cuts at: 0 1126 1887
Size: 1126 761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 1504 1887
Size: 18 1486 383
Fragments arranged by size:
1486 383 18
Bpu10I CC'TnA_GC
Cuts at: 0 1126 1887
Size: 1126 761
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 976 1603 1887
Size: 508 468 627 284
Fragments arranged by size:
627 508 468 284
BsaBI GATnn'nnATC
Cuts at: 0 1400 1887
Size: 1400 487
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 233 1887
Size: 233 1654
BsrDI GCAATG_nn'
Cuts at: 0 523 1743 1887
Size: 523 1220 144
Fragments arranged by size:
1220 523 144
BstAPI GCAn_nnn'nTGC
Cuts at: 0 816 1887
Size: 816 1071
BstEII G'GTnAC_C
Cuts at: 0 763 1887
Size: 763 1124
BstXI CCAn_nnnn'nTGG
Cuts at: 0 959 1632 1887
Size: 959 673 255
Fragments arranged by size:
959 673 255
BstYI r'GATC_y
Cuts at: 0 1431 1887
Size: 1431 456
BtgI C'CryG_G
Cuts at: 0 749 899 1887
Size: 749 150 988
Fragments arranged by size:
988 749 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 35 1887
Size: 35 1852
EarI CTCTTCn'nnn_
Cuts at: 0 86 194 1887
Size: 86 108 1693
Fragments arranged by size:
1693 108 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 38 110 175 1504 1887
Size: 18 20 72 65 1329 383
Fragments arranged by size:
1329 383 72 65 20 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1201 1887
Size: 1201 686
EcoO109I rG'GnC_Cy
Cuts at: 0 596 609 1081 1798 1887
Size: 596 13 472 717 89
Fragments arranged by size:
717 596 472 89 13
EcoRV GAT'ATC
Cuts at: 0 1518 1887
Size: 1518 369
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 193 1180 1887
Size: 193 987 707
Fragments arranged by size:
987 707 193
HincII GTy'rAC
Cuts at: 0 552 782 1490 1822 1887
Size: 552 230 708 332 65
Fragments arranged by size:
708 552 332 230 65
HpaI GTT'AAC
Cuts at: 0 782 1887
Size: 782 1105
NcoI C'CATG_G
Cuts at: 0 749 899 1887
Size: 749 150 988
Fragments arranged by size:
988 749 150
NdeI CA'TA_TG
Cuts at: 0 1559 1887
Size: 1559 328
NspI r_CATG'y
Cuts at: 0 199 819 1502 1571 1828 1887
Size: 199 620 683 69 257 59
Fragments arranged by size:
683 620 257 199 69 59
PciI A'CATG_T
Cuts at: 0 1824 1887
Size: 1824 63
PpuMI rG'GwC_Cy
Cuts at: 0 596 609 1081 1798 1887
Size: 596 13 472 717 89
Fragments arranged by size:
717 596 472 89 13
PsiI TTA'TAA
Cuts at: 0 383 1887
Size: 383 1504
PstI C_TGCA'G
Cuts at: 0 1345 1887
Size: 1345 542
SanDI GG'GwC_CC
Cuts at: 0 596 609 1798 1887
Size: 596 13 1189 89
Fragments arranged by size:
1189 596 89 13
SapI GCTCTTCn'nnn_
Cuts at: 0 194 1887
Size: 194 1693
SfcI C'TryA_G
Cuts at: 0 1018 1341 1887
Size: 1018 323 546
Fragments arranged by size:
1018 546 323
SmlI C'TyrA_G
Cuts at: 0 416 523 991 1582 1887
Size: 416 107 468 591 305
Fragments arranged by size:
591 468 416 305 107
SphI G_CATG'C
Cuts at: 0 199 1887
Size: 199 1688
SspI AAT'ATT
Cuts at: 0 101 1887
Size: 101 1786
StyI C'CwwG_G
Cuts at: 0 749 899 958 1887
Size: 749 150 59 929
Fragments arranged by size:
929 749 150 59
TatI w'GTAC_w
Cuts at: 0 872 1887
Size: 872 1015
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 441 1809 1887
Size: 441 1368 78
Fragments arranged by size:
1368 441 78
XhoI C'TCGA_G
Cuts at: 0 416 1887
Size: 416 1471
Enzymes that do cut:
AccI AcuI AflIII AlfI ApoI AseI AvaI BbsI
BbvCI BpmI Bpu10I BpuEI BsaBI BseRI BsrDI BstAPI
BstEII BstXI BstYI BtgI BtgZI EarI Eco57MI EcoNI
EcoO109I EcoRV FalI HincII HpaI NcoI NdeI NspI
PciI PpuMI PsiI PstI SanDI SapI SfcI SmlI
SphI SspI StyI TatI XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII
BaeI BamHI BanI BanII BbeI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstZ17I
Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoICRI EcoRI FseI FspI FspAI HaeII
Hin4I HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI
PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TaqII
TstI Tth111I XbaI XmaI XmnI ZraI