Restriction map for
pF1KB8433
(Linear) (Six-base) MAPSORT of: KIBB8433.seq Check: 6225 from: 1 to: 849
KIBB8433.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 213 849
Size: 213 636
Acc65I G'GTAC_C
Cuts at: 0 395 849
Size: 395 454
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 298 661 849
Size: 109 189 363 188
Fragments arranged by size:
363 189 188 109
AfeI AGC'GCT
Cuts at: 0 838 849
Size: 838 11
AflIII A'CryG_T
Cuts at: 0 449 849
Size: 449 400
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 235 849
Size: 235 614
AlwNI CAG_nnn'CTG
Cuts at: 0 156 246 549 849
Size: 156 90 303 300
Fragments arranged by size:
303 300 156 90
AvaI C'yCGr_G
Cuts at: 0 341 849
Size: 341 508
BanI G'GyrC_C
Cuts at: 0 395 849
Size: 395 454
BanII G_rGCy'C
Cuts at: 0 405 730 770 849
Size: 405 325 40 79
Fragments arranged by size:
405 325 79 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 325 849
Size: 325 524
Bme1580I G_kGCm'C
Cuts at: 0 556 849
Size: 556 293
BmrI ACTGGGnnnn_n'
Cuts at: 0 352 554 849
Size: 352 202 295
Fragments arranged by size:
352 295 202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 190 223 742 849
Size: 136 54 33 519 107
Fragments arranged by size:
519 136 107 54 33
Bpu10I CC'TnA_GC
Cuts at: 0 200 849
Size: 200 649
BsaWI w'CCGG_w
Cuts at: 0 617 849
Size: 617 232
BseYI C'CCAG_C
Cuts at: 0 549 810 849
Size: 549 261 39
Fragments arranged by size:
549 261 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 562 670 849
Size: 232 330 108 179
Fragments arranged by size:
330 232 179 108
BsiEI CG_ry'CG
Cuts at: 0 710 849
Size: 710 139
BsiHKAI G_wGCw'C
Cuts at: 0 230 730 770 849
Size: 230 500 40 79
Fragments arranged by size:
500 230 79 40
Bsp1286I G_dGCh'C
Cuts at: 0 230 405 556 730 770 849
Size: 230 175 151 174 40 79
Fragments arranged by size:
230 175 174 151 79 40
BspEI T'CCGG_A
Cuts at: 0 617 849
Size: 617 232
BspHI T'CATG_A
Cuts at: 0 296 849
Size: 296 553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 213 849
Size: 213 636
BstXI CCAn_nnnn'nTGG
Cuts at: 0 168 849
Size: 168 681
BstYI r'GATC_y
Cuts at: 0 518 849
Size: 518 331
BtgI C'CryG_G
Cuts at: 0 655 849
Size: 655 194
EaeI y'GGCC_r
Cuts at: 0 158 473 574 603 707 849
Size: 158 315 101 29 104 142
Fragments arranged by size:
315 158 142 104 101 29
EagI C'GGCC_G
Cuts at: 0 707 849
Size: 707 142
EarI CTCTTCn'nnn_
Cuts at: 0 503 849
Size: 503 346
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 49 849
Size: 49 800
EcoICRI GAG'CTC
Cuts at: 0 728 768 849
Size: 728 40 81
Fragments arranged by size:
728 81 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 136 190 223 298 661 742 849
Size: 109 27 54 33 75 363 81 107
Fragments arranged by size:
363 109 107 81 75 54 33 27
EcoO109I rG'GnC_Cy
Cuts at: 0 153 234 273 849
Size: 153 81 39 576
Fragments arranged by size:
576 153 81 39
HaeII r_GCGC'y
Cuts at: 0 840 849
Size: 840 9
KpnI G_GTAC'C
Cuts at: 0 399 849
Size: 399 450
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 644 849
Size: 311 333 205
Fragments arranged by size:
333 311 205
MscI TGG'CCA
Cuts at: 0 160 849
Size: 160 689
MslI CAynn'nnrTG
Cuts at: 0 448 849
Size: 448 401
MspA1I CmG'CkG
Cuts at: 0 325 546 849
Size: 325 221 303
Fragments arranged by size:
325 303 221
NcoI C'CATG_G
Cuts at: 0 655 849
Size: 655 194
NspI r_CATG'y
Cuts at: 0 453 849
Size: 453 396
PciI A'CATG_T
Cuts at: 0 449 849
Size: 449 400
PfoI T'CCnGG_A
Cuts at: 0 167 849
Size: 167 682
PpuMI rG'GwC_Cy
Cuts at: 0 153 273 849
Size: 153 120 576
Fragments arranged by size:
576 153 120
PstI C_TGCA'G
Cuts at: 0 224 849
Size: 224 625
PvuII CAG'CTG
Cuts at: 0 546 849
Size: 546 303
SacI G_AGCT'C
Cuts at: 0 730 770 849
Size: 730 40 79
Fragments arranged by size:
730 79 40
SfcI C'TryA_G
Cuts at: 0 220 849
Size: 220 629
SmaI CCC'GGG
Cuts at: 0 343 849
Size: 343 506
SrfI GCCC'GGGC
Cuts at: 0 343 849
Size: 343 506
StuI AGG'CCT
Cuts at: 0 495 849
Size: 495 354
StyI C'CwwG_G
Cuts at: 0 377 655 849
Size: 377 278 194
Fragments arranged by size:
377 278 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 316 849
Size: 316 533
TatI w'GTAC_w
Cuts at: 0 608 849
Size: 608 241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 594 849
Size: 594 255
XmaI C'CCGG_G
Cuts at: 0 341 849
Size: 341 508
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AlfI AlwNI AvaI
BanI BanII BbsI Bme1580I BmrI BpmI Bpu10I BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BstXI BstYI BtgI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I HaeII KpnI MmeI MscI MslI MspA1I
NcoI NspI PciI PfoI PpuMI PstI PvuII SacI
SfcI SmaI SrfI StuI StyI TaqII TatI XcmI
XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AhdI AleI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BsiWI BsmI BsmBI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KasI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SspI SwaI TstI Tth111I XbaI
XhoI XmnI ZraI