Restriction map for
pF1KB8434
(Linear) (Six-base) MAPSORT of: KIBB8434.seq Check: 6646 from: 1 to: 414
KIBB8434.orf
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
Acc65I G'GTAC_C
Cuts at: 0 389 414
Size: 389 25
AgeI A'CCGG_T
Cuts at: 0 246 414
Size: 246 168
AleI CACnn'nnGTG
Cuts at: 0 286 414
Size: 286 128
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 275 414
Size: 275 139
AlwNI CAG_nnn'CTG
Cuts at: 0 99 229 414
Size: 99 130 185
Fragments arranged by size:
185 130 99
AvaI C'yCGr_G
Cuts at: 0 263 414
Size: 263 151
BamHI G'GATC_C
Cuts at: 0 114 414
Size: 114 300
BanI G'GyrC_C
Cuts at: 0 389 414
Size: 389 25
BanII G_rGCy'C
Cuts at: 0 215 414
Size: 215 199
BglI GCCn_nnn'nGGC
Cuts at: 0 181 414
Size: 181 233
BmgBI CAC'GTC
Cuts at: 0 321 414
Size: 321 93
BmrI ACTGGGnnnn_n'
Cuts at: 0 20 414
Size: 20 394
BsaWI w'CCGG_w
Cuts at: 0 246 414
Size: 246 168
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 376 414
Size: 22 354 38
Fragments arranged by size:
354 38 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 414
Size: 280 134
BsiEI CG_ry'CG
Cuts at: 0 247 414
Size: 247 167
Bsp1286I G_dGCh'C
Cuts at: 0 215 414
Size: 215 199
BspHI T'CATG_A
Cuts at: 0 158 414
Size: 158 256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 401 414
Size: 401 13
BsrBI CCG'CTC
Cuts at: 0 366 414
Size: 366 48
BsrFI r'CCGG_y
Cuts at: 0 106 246 414
Size: 106 140 168
Fragments arranged by size:
168 140 106
BssHII G'CGCG_C
Cuts at: 0 144 414
Size: 144 270
BstAPI GCAn_nnn'nTGC
Cuts at: 0 229 414
Size: 229 185
BstYI r'GATC_y
Cuts at: 0 114 414
Size: 114 300
BtgI C'CryG_G
Cuts at: 0 62 201 347 404 414
Size: 62 139 146 57 10
Fragments arranged by size:
146 139 62 57 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 56 341 414
Size: 56 285 73
Fragments arranged by size:
285 73 56
EaeI y'GGCC_r
Cuts at: 0 53 356 414
Size: 53 303 58
Fragments arranged by size:
303 58 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 199 414
Size: 199 215
FspI TGC'GCA
Cuts at: 0 222 397 414
Size: 222 175 17
Fragments arranged by size:
222 175 17
HaeII r_GCGC'y
Cuts at: 0 62 176 414
Size: 62 114 238
Fragments arranged by size:
238 114 62
HincII GTy'rAC
Cuts at: 0 129 414
Size: 129 285
KpnI G_GTAC'C
Cuts at: 0 393 414
Size: 393 21
MscI TGG'CCA
Cuts at: 0 55 414
Size: 55 359
MslI CAynn'nnrTG
Cuts at: 0 163 286 414
Size: 163 123 128
Fragments arranged by size:
163 128 123
MspA1I CmG'CkG
Cuts at: 0 64 414
Size: 64 350
NaeI GCC'GGC
Cuts at: 0 108 414
Size: 108 306
NgoMIV G'CCGG_C
Cuts at: 0 106 414
Size: 106 308
NspI r_CATG'y
Cuts at: 0 24 414
Size: 24 390
PfoI T'CCnGG_A
Cuts at: 0 98 275 414
Size: 98 177 139
Fragments arranged by size:
177 139 98
SacII CC_GC'GG
Cuts at: 0 65 414
Size: 65 349
SmaI CCC'GGG
Cuts at: 0 265 414
Size: 265 149
SpeI A'CTAG_T
Cuts at: 0 75 414
Size: 75 339
SphI G_CATG'C
Cuts at: 0 24 414
Size: 24 390
XmaI C'CCGG_G
Cuts at: 0 263 414
Size: 263 151
Enzymes that do cut:
Acc65I AgeI AleI AlfI AlwNI AvaI BamHI BanI
BanII BglI BmgBI BmrI BsaWI BseRI BsgI BsiEI
Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BstAPI BstYI
BtgI BtgZI EaeI EciI FspI HaeII HincII KpnI
MscI MslI MspA1I NaeI NgoMIV NspI PfoI SacII
SmaI SpeI SphI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AhdI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseYI BsiHKAI
BsiWI BsmI BsmBI BspEI BsrDI BsrGI BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DraIII DrdI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspAI Hin4I HindIII HpaI
KasI MfeI MluI MmeI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmlI SnaBI SrfI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI