(Linear) (Six-base) MAPSORT of: KIBB8442.seq  Check: 1948  from: 1  to: 378



KIBB8442.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     378 
   Size:        175     203
 
AvaI C'yCGr_G

Cuts at:      0      92     378 
   Size:         92     286
 
BamHI G'GATC_C

Cuts at:      0     121     378 
   Size:        121     257
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      40     378 
   Size:         40     338
 
BsaWI w'CCGG_w

Cuts at:      0     209     378 
   Size:        209     169
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      32     378 
   Size:         32     346
 
BseYI C'CCAG_C

Cuts at:      0     105     378 
   Size:        105     273
 
BsmI GAATG_Cn'

Cuts at:      0      72     378 
   Size:         72     306
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      77     378 
   Size:         77     301
 
BstYI r'GATC_y

Cuts at:      0     121     378 
   Size:        121     257
 
BtsI GCAGTG_nn'

Cuts at:      0     314     378 
   Size:        314      64
 
ClaI AT'CG_AT

Cuts at:      0      84     378 
   Size:         84     294
 
EaeI y'GGCC_r

Cuts at:      0     168     378 
   Size:        168     210
 
EarI CTCTTCn'nnn_

Cuts at:      0     251     364     378 
   Size:        251     113      14

  Fragments arranged by size: 

                 251     113      14
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     252     378 
   Size:        252     126
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     378 
   Size:        175     203
 
HaeII r_GCGC'y

Cuts at:      0     228     378 
   Size:        228     150
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      27      59     378 
   Size:         27      32     319

  Fragments arranged by size: 

                 319      32      27
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     230     378 
   Size:         89     141     148

  Fragments arranged by size: 

                 148     141      89
 
MscI TGG'CCA

Cuts at:      0     170     378 
   Size:        170     208
 
NspI r_CATG'y

Cuts at:      0     303     378 
   Size:        303      75
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     249     333     378 
   Size:        249      84      45

  Fragments arranged by size: 

                 249      84      45
 
SmaI CCC'GGG

Cuts at:      0      94     378 
   Size:         94     284
 
XmaI C'CCGG_G

Cuts at:      0      92     378 
   Size:         92     286
 
XmnI GAAnn'nnTTC

Cuts at:      0      19     378 
   Size:         19     359
 

 Enzymes that do cut:

     AcuI     AvaI    BamHI     BbsI    BsaWI    BseRI    BseYI     BsmI
    BspMI    BstYI     BtsI     ClaI     EaeI     EarI     EciI  Eco57MI
    HaeII    Hin4I     MmeI     MscI     NspI    PflMI     SmaI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI    CspCI     DraI   DraIII     DrdI
     EagI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     ZraI