Restriction map for
pF1KB8443
(Linear) (Six-base) MAPSORT of: KIBB8443.seq Check: 1734 from: 1 to: 1758
KIBB8443.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 1328 1456 1496 1570 1758
Size: 280 1048 128 40 74 188
Fragments arranged by size:
1048 280 188 128 74 40
AflII C'TTAA_G
Cuts at: 0 1569 1758
Size: 1569 189
AflIII A'CryG_T
Cuts at: 0 853 1758
Size: 853 905
AlwNI CAG_nnn'CTG
Cuts at: 0 50 328 1758
Size: 50 278 1430
Fragments arranged by size:
1430 278 50
ApaI G_GGCC'C
Cuts at: 0 294 1758
Size: 294 1464
ApoI r'AATT_y
Cuts at: 0 953 1758
Size: 953 805
AvaI C'yCGr_G
Cuts at: 0 5 1758
Size: 5 1753
BamHI G'GATC_C
Cuts at: 0 1536 1758
Size: 1536 222
BanI G'GyrC_C
Cuts at: 0 419 814 1302 1714 1758
Size: 419 395 488 412 44
Fragments arranged by size:
488 419 412 395 44
BanII G_rGCy'C
Cuts at: 0 184 282 294 1758
Size: 184 98 12 1464
Fragments arranged by size:
1464 184 98 12
BbeI G_GCGC'C
Cuts at: 0 1306 1758
Size: 1306 452
BbsI GAAGACnn'nnnn_
Cuts at: 0 1601 1758
Size: 1601 157
BbvCI CC'TCA_GC
Cuts at: 0 384 1758
Size: 384 1374
BciVI GTATCCnnnnn_n'
Cuts at: 0 1409 1758
Size: 1409 349
BclI T'GATC_A
Cuts at: 0 750 1758
Size: 750 1008
BglI GCCn_nnn'nGGC
Cuts at: 0 277 1758
Size: 277 1481
BglII A'GATC_T
Cuts at: 0 1617 1758
Size: 1617 141
BlpI GC'TnA_GC
Cuts at: 0 26 1758
Size: 26 1732
Bme1580I G_kGCm'C
Cuts at: 0 294 1241 1758
Size: 294 947 517
Fragments arranged by size:
947 517 294
BmrI ACTGGGnnnn_n'
Cuts at: 0 72 1758
Size: 72 1686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 147 1380 1758
Size: 129 18 1233 378
Fragments arranged by size:
1233 378 129 18
Bpu10I CC'TnA_GC
Cuts at: 0 384 1758
Size: 384 1374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1184 1409 1758
Size: 337 847 225 349
Fragments arranged by size:
847 349 337 225
BsaI GGTCTCn'nnnn_
Cuts at: 0 1252 1714 1758
Size: 1252 462 44
Fragments arranged by size:
1252 462 44
BsaHI Gr'CG_yC
Cuts at: 0 1303 1758
Size: 1303 455
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 81 84 111 271 1758
Size: 81 3 27 160 1487
Fragments arranged by size:
1487 160 81 27 3
BseYI C'CCAG_C
Cuts at: 0 400 924 1333 1667 1734 1758
Size: 400 524 409 334 67 24
Fragments arranged by size:
524 409 400 334 67 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1153 1758
Size: 1153 605
BsiHKAI G_wGCw'C
Cuts at: 0 184 1758
Size: 184 1574
Bsp1286I G_dGCh'C
Cuts at: 0 184 282 294 1241 1758
Size: 184 98 12 947 517
Fragments arranged by size:
947 517 184 98 12
BspHI T'CATG_A
Cuts at: 0 1143 1268 1758
Size: 1143 125 490
Fragments arranged by size:
1143 490 125
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1105 1758
Size: 1105 653
BsrDI GCAATG_nn'
Cuts at: 0 596 630 1758
Size: 596 34 1128
Fragments arranged by size:
1128 596 34
BstXI CCAn_nnnn'nTGG
Cuts at: 0 400 1251 1299 1758
Size: 400 851 48 459
Fragments arranged by size:
851 459 400 48
BstYI r'GATC_y
Cuts at: 0 1536 1617 1758
Size: 1536 81 141
Fragments arranged by size:
1536 141 81
Bsu36I CC'TnA_GG
Cuts at: 0 224 239 254 767 1758
Size: 224 15 15 513 991
Fragments arranged by size:
991 513 224 15 15
BtgI C'CryG_G
Cuts at: 0 63 1579 1758
Size: 63 1516 179
Fragments arranged by size:
1516 179 63
BtsI GCAGTG_nn'
Cuts at: 0 51 511 1758
Size: 51 460 1247
Fragments arranged by size:
1247 460 51
DrdI GACnn_nn'nnGTC
Cuts at: 0 1428 1758
Size: 1428 330
EaeI y'GGCC_r
Cuts at: 0 330 430 1758
Size: 330 100 1328
Fragments arranged by size:
1328 330 100
EarI CTCTTCn'nnn_
Cuts at: 0 1199 1597 1758
Size: 1199 398 161
Fragments arranged by size:
1199 398 161
EcoICRI GAG'CTC
Cuts at: 0 182 1758
Size: 182 1576
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 147 280 1328 1380 1456 1496 1570
Size: 129 18 133 1048 52 76 40 74
Cuts at: 1570 1758
Size: 188
Fragments arranged by size:
1048 188 133 129 76 74 52 40
18
EcoO109I rG'GnC_Cy
Cuts at: 0 291 580 1758
Size: 291 289 1178
Fragments arranged by size:
1178 291 289
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 573 1758
Size: 573 1185
HaeII r_GCGC'y
Cuts at: 0 1306 1734 1758
Size: 1306 428 24
Fragments arranged by size:
1306 428 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 310 342 408 440 739 771
Size: 181 32 97 32 66 32 299 32
Cuts at: 771 1609 1641 1758
Size: 838 32 117
Fragments arranged by size:
838 299 181 117 97 66 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 1302 1758
Size: 1302 456
MscI TGG'CCA
Cuts at: 0 332 1758
Size: 332 1426
MspA1I CmG'CkG
Cuts at: 0 1738 1758
Size: 1738 20
NarI GG'CG_CC
Cuts at: 0 1303 1758
Size: 1303 455
NcoI C'CATG_G
Cuts at: 0 63 1758
Size: 63 1695
NspI r_CATG'y
Cuts at: 0 857 1758
Size: 857 901
PasI CC'CwG_GG
Cuts at: 0 105 275 1758
Size: 105 170 1483
Fragments arranged by size:
1483 170 105
PciI A'CATG_T
Cuts at: 0 853 1758
Size: 853 905
PpuMI rG'GwC_Cy
Cuts at: 0 580 1758
Size: 580 1178
PsiI TTA'TAA
Cuts at: 0 1219 1758
Size: 1219 539
PspOMI G'GGCC_C
Cuts at: 0 290 1758
Size: 290 1468
PvuII CAG'CTG
Cuts at: 0 1738 1758
Size: 1738 20
SacI G_AGCT'C
Cuts at: 0 184 1758
Size: 184 1574
SfcI C'TryA_G
Cuts at: 0 743 1758
Size: 743 1015
SfoI GGC'GCC
Cuts at: 0 1304 1758
Size: 1304 454
SmlI C'TyrA_G
Cuts at: 0 316 1199 1424 1569 1758
Size: 316 883 225 145 189
Fragments arranged by size:
883 316 225 189 145
SpeI A'CTAG_T
Cuts at: 0 1678 1758
Size: 1678 80
SspI AAT'ATT
Cuts at: 0 496 1758
Size: 496 1262
StyI C'CwwG_G
Cuts at: 0 63 84 1758
Size: 63 21 1674
Fragments arranged by size:
1674 63 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 396 428 1758
Size: 396 32 1330
Fragments arranged by size:
1330 396 32
Tth111I GACn'n_nGTC
Cuts at: 0 195 324 1513 1758
Size: 195 129 1189 245
Fragments arranged by size:
1189 245 195 129
Enzymes that do cut:
AcuI AflII AflIII AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI
BsrDI BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI
EarI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I KasI
MscI MspA1I NarI NcoI NspI PasI PciI PpuMI
PsiI PspOMI PvuII SacI SfcI SfoI SmlI SpeI
SspI StyI TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BcgI BfrBI BmgBI BmtI BplI BsaAI BsaBI
BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DraIII EagI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MslI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PflMI PfoI PmeI
PmlI PpiI PshAI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SphI SrfI StuI SwaI
TaqII TatI XbaI XcmI XhoI XmaI XmnI ZraI