Restriction map for
pF1KB8445
(Linear) (Six-base) MAPSORT of: KIBB8445.seq Check: 4456 from: 1 to: 903
KIBB8445.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 555 623 903
Size: 555 68 280
Fragments arranged by size:
555 280 68
AccI GT'mk_AC
Cuts at: 0 449 903
Size: 449 454
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 903
Size: 749 154
AfeI AGC'GCT
Cuts at: 0 85 889 903
Size: 85 804 14
Fragments arranged by size:
804 85 14
AhdI GACnn_n'nnGTC
Cuts at: 0 489 903
Size: 489 414
AlwNI CAG_nnn'CTG
Cuts at: 0 390 903
Size: 390 513
ApaI G_GGCC'C
Cuts at: 0 9 433 903
Size: 9 424 470
Fragments arranged by size:
470 424 9
AvaI C'yCGr_G
Cuts at: 0 106 903
Size: 106 797
BanII G_rGCy'C
Cuts at: 0 9 115 433 726 903
Size: 9 106 318 293 177
Fragments arranged by size:
318 293 177 106 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 891 903
Size: 891 12
BlpI GC'TnA_GC
Cuts at: 0 13 903
Size: 13 890
Bme1580I G_kGCm'C
Cuts at: 0 9 433 903
Size: 9 424 470
Fragments arranged by size:
470 424 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 37 428 903
Size: 37 391 475
Fragments arranged by size:
475 391 37
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 389 412 685 903
Size: 276 113 23 273 218
Fragments arranged by size:
276 273 218 113 23
Bpu10I CC'TnA_GC
Cuts at: 0 659 903
Size: 659 244
BsaAI yAC'GTr
Cuts at: 0 870 903
Size: 870 33
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 778 811 814 903
Size: 778 33 3 89
Fragments arranged by size:
778 89 33 3
BseYI C'CCAG_C
Cuts at: 0 8 200 903
Size: 8 192 703
Fragments arranged by size:
703 192 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 903
Size: 674 229
BsiEI CG_ry'CG
Cuts at: 0 147 903
Size: 147 756
BsiHKAI G_wGCw'C
Cuts at: 0 38 903
Size: 38 865
Bsp1286I G_dGCh'C
Cuts at: 0 9 38 115 433 726 903
Size: 9 29 77 318 293 177
Fragments arranged by size:
318 293 177 77 29 9
BspHI T'CATG_A
Cuts at: 0 68 581 903
Size: 68 513 322
Fragments arranged by size:
513 322 68
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 555 623 903
Size: 555 68 280
Fragments arranged by size:
555 280 68
BsrGI T'GTAC_A
Cuts at: 0 56 903
Size: 56 847
BtgI C'CryG_G
Cuts at: 0 881 903
Size: 881 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 311 903
Size: 311 592
DraIII CAC_nnn'GTG
Cuts at: 0 606 608 903
Size: 606 2 295
Fragments arranged by size:
606 295 2
DrdI GACnn_nn'nnGTC
Cuts at: 0 516 903
Size: 516 387
EaeI y'GGCC_r
Cuts at: 0 225 744 903
Size: 225 519 159
Fragments arranged by size:
519 225 159
EarI CTCTTCn'nnn_
Cuts at: 0 43 903
Size: 43 860
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 389 412 685 749 903
Size: 276 113 23 273 64 154
Fragments arranged by size:
276 273 154 113 64 23
EcoO109I rG'GnC_Cy
Cuts at: 0 5 429 430 588 903
Size: 5 424 1 158 315
Fragments arranged by size:
424 315 158 5 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 354 903
Size: 354 549
HaeII r_GCGC'y
Cuts at: 0 87 177 891 903
Size: 87 90 714 12
Fragments arranged by size:
714 90 87 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 475 507 903
Size: 475 32 396
Fragments arranged by size:
475 396 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 501 518 903
Size: 501 17 385
Fragments arranged by size:
501 385 17
MslI CAynn'nnrTG
Cuts at: 0 556 903
Size: 556 347
MspA1I CmG'CkG
Cuts at: 0 132 367 807 903
Size: 132 235 440 96
Fragments arranged by size:
440 235 132 96
NdeI CA'TA_TG
Cuts at: 0 29 903
Size: 29 874
NspI r_CATG'y
Cuts at: 0 402 903
Size: 402 501
PfoI T'CCnGG_A
Cuts at: 0 773 903
Size: 773 130
PmlI CAC'GTG
Cuts at: 0 870 903
Size: 870 33
PpuMI rG'GwC_Cy
Cuts at: 0 588 903
Size: 588 315
PspOMI G'GGCC_C
Cuts at: 0 5 429 903
Size: 5 424 474
Fragments arranged by size:
474 424 5
PstI C_TGCA'G
Cuts at: 0 566 903
Size: 566 337
SapI GCTCTTCn'nnn_
Cuts at: 0 43 903
Size: 43 860
SexAI A'CCwGG_T
Cuts at: 0 263 320 903
Size: 263 57 583
Fragments arranged by size:
583 263 57
SfcI C'TryA_G
Cuts at: 0 562 903
Size: 562 341
SmlI C'TyrA_G
Cuts at: 0 106 903
Size: 106 797
SphI G_CATG'C
Cuts at: 0 402 903
Size: 402 501
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 300 364 649 846 903
Size: 300 64 285 197 57
Fragments arranged by size:
300 285 197 64 57
TatI w'GTAC_w
Cuts at: 0 56 903
Size: 56 847
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 821 853 903
Size: 821 32 50
Fragments arranged by size:
821 50 32
Tth111I GACn'n_nGTC
Cuts at: 0 322 903
Size: 322 581
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 441 903
Size: 441 462
XhoI C'TCGA_G
Cuts at: 0 106 903
Size: 106 797
XmnI GAAnn'nnTTC
Cuts at: 0 76 903
Size: 76 827
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI AlwNI ApaI AvaI
BanII BbsI BlpI Bme1580I BmrI BpmI Bpu10I BsaAI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrGI BtgI BtgZI DraIII DrdI EaeI EarI Eco57MI
EcoO109I FalI HaeII Hin4I MmeI MslI MspA1I NdeI
NspI PfoI PmlI PpuMI PspOMI PstI SapI SexAI
SfcI SmlI SphI TaqII TatI TstI Tth111I XcmI
XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BmtI BplI BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI StyI SwaI XbaI
XmaI ZraI