Restriction map for
pF1KB8446
(Linear) (Six-base) MAPSORT of: KIBB8446.seq Check: 8002 from: 1 to: 927
KIBB8446.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AflII C'TTAA_G
Cuts at: 0 148 927
Size: 148 779
AlwNI CAG_nnn'CTG
Cuts at: 0 396 927
Size: 396 531
ApaI G_GGCC'C
Cuts at: 0 590 927
Size: 590 337
ApoI r'AATT_y
Cuts at: 0 121 214 253 927
Size: 121 93 39 674
Fragments arranged by size:
674 121 93 39
BanII G_rGCy'C
Cuts at: 0 590 747 807 927
Size: 590 157 60 120
Fragments arranged by size:
590 157 120 60
BbvCI CC'TCA_GC
Cuts at: 0 682 823 927
Size: 682 141 104
Fragments arranged by size:
682 141 104
BglII A'GATC_T
Cuts at: 0 156 927
Size: 156 771
Bme1580I G_kGCm'C
Cuts at: 0 590 927
Size: 590 337
BmrI ACTGGGnnnn_n'
Cuts at: 0 729 927
Size: 729 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 658 673 927
Size: 373 285 15 254
Fragments arranged by size:
373 285 254 15
Bpu10I CC'TnA_GC
Cuts at: 0 682 823 927
Size: 682 141 104
Fragments arranged by size:
682 141 104
BsaI GGTCTCn'nnnn_
Cuts at: 0 704 927
Size: 704 223
BsaWI w'CCGG_w
Cuts at: 0 38 927
Size: 38 889
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 535 565 927
Size: 535 30 362
Fragments arranged by size:
535 362 30
BsiHKAI G_wGCw'C
Cuts at: 0 807 927
Size: 807 120
BsmI GAATG_Cn'
Cuts at: 0 212 927
Size: 212 715
Bsp1286I G_dGCh'C
Cuts at: 0 590 747 807 927
Size: 590 157 60 120
Fragments arranged by size:
590 157 120 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 554 803 927
Size: 554 249 124
Fragments arranged by size:
554 249 124
BsrBI CCG'CTC
Cuts at: 0 768 927
Size: 768 159
BsrFI r'CCGG_y
Cuts at: 0 725 927
Size: 725 202
BstAPI GCAn_nnn'nTGC
Cuts at: 0 424 846 927
Size: 424 422 81
Fragments arranged by size:
424 422 81
BstXI CCAn_nnnn'nTGG
Cuts at: 0 582 927
Size: 582 345
BstYI r'GATC_y
Cuts at: 0 156 644 927
Size: 156 488 283
Fragments arranged by size:
488 283 156
BtgI C'CryG_G
Cuts at: 0 887 927
Size: 887 40
BtsI GCAGTG_nn'
Cuts at: 0 781 927
Size: 781 146
DraI TTT'AAA
Cuts at: 0 233 927
Size: 233 694
DraIII CAC_nnn'GTG
Cuts at: 0 597 927
Size: 597 330
EaeI y'GGCC_r
Cuts at: 0 830 927
Size: 830 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 637 744 927
Size: 637 107 183
Fragments arranged by size:
637 183 107
EcoICRI GAG'CTC
Cuts at: 0 805 927
Size: 805 122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 658 673 927
Size: 373 285 15 254
Fragments arranged by size:
373 285 254 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 832 927
Size: 832 95
EcoO109I rG'GnC_Cy
Cuts at: 0 339 543 586 587 927
Size: 339 204 43 1 340
Fragments arranged by size:
340 339 204 43 1
EcoRV GAT'ATC
Cuts at: 0 200 927
Size: 200 727
FspI TGC'GCA
Cuts at: 0 333 799 927
Size: 333 466 128
Fragments arranged by size:
466 333 128
HaeII r_GCGC'y
Cuts at: 0 705 927
Size: 705 222
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 535 567 927
Size: 535 32 360
Fragments arranged by size:
535 360 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 158 927
Size: 111 47 769
Fragments arranged by size:
769 111 47
MscI TGG'CCA
Cuts at: 0 832 927
Size: 832 95
MspA1I CmG'CkG
Cuts at: 0 611 636 927
Size: 611 25 291
Fragments arranged by size:
611 291 25
PflMI CCAn_nnn'nTGG
Cuts at: 0 396 597 927
Size: 396 201 330
Fragments arranged by size:
396 330 201
PfoI T'CCnGG_A
Cuts at: 0 671 689 927
Size: 671 18 238
Fragments arranged by size:
671 238 18
PpuMI rG'GwC_Cy
Cuts at: 0 543 927
Size: 543 384
PspOMI G'GGCC_C
Cuts at: 0 586 927
Size: 586 341
PstI C_TGCA'G
Cuts at: 0 365 927
Size: 365 562
PvuII CAG'CTG
Cuts at: 0 636 927
Size: 636 291
SacI G_AGCT'C
Cuts at: 0 807 927
Size: 807 120
SfcI C'TryA_G
Cuts at: 0 66 361 927
Size: 66 295 566
Fragments arranged by size:
566 295 66
SmlI C'TyrA_G
Cuts at: 0 148 927
Size: 148 779
StyI C'CwwG_G
Cuts at: 0 581 833 927
Size: 581 252 94
Fragments arranged by size:
581 252 94
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 465 927
Size: 465 462
XmnI GAAnn'nnTTC
Cuts at: 0 253 439 927
Size: 253 186 488
Fragments arranged by size:
488 253 186
Enzymes that do cut:
AflII AlwNI ApaI ApoI BanII BbvCI BglII Bme1580I
BmrI BpmI Bpu10I BsaI BsaWI BsaXI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI BstYI BtgI
BtsI DraI DraIII EaeI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FspI HaeII Hin4I MmeI MscI MspA1I
PflMI PfoI PpuMI PspOMI PstI PvuII SacI SfcI
SmlI StyI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BcgI BciVI BclI BfrBI BglI BlpI BmgBI BmtI
BplI BpuEI BsaAI BsaBI BsaHI BseRI BseYI BsgI
BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DrdI EagI EarI EcoRI FalI FseI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmaI ZraI