Restriction map for
pF1KB8453
(Linear) (Six-base) MAPSORT of: KIBB8453.seq Check: 5917 from: 1 to: 963
KIBB8453.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AatII G_ACGT'C
Cuts at: 0 494 963
Size: 494 469
Acc65I G'GTAC_C
Cuts at: 0 74 963
Size: 74 889
AflIII A'CryG_T
Cuts at: 0 227 963
Size: 227 736
AleI CACnn'nnGTG
Cuts at: 0 939 963
Size: 939 24
AlwNI CAG_nnn'CTG
Cuts at: 0 465 786 963
Size: 465 321 177
Fragments arranged by size:
465 321 177
ApaI G_GGCC'C
Cuts at: 0 143 871 963
Size: 143 728 92
Fragments arranged by size:
728 143 92
AvaI C'yCGr_G
Cuts at: 0 115 135 188 963
Size: 115 20 53 775
Fragments arranged by size:
775 115 53 20
BanI G'GyrC_C
Cuts at: 0 74 955 963
Size: 74 881 8
Fragments arranged by size:
881 74 8
BanII G_rGCy'C
Cuts at: 0 36 62 143 871 963
Size: 36 26 81 728 92
Fragments arranged by size:
728 92 81 36 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 159 187 255 963
Size: 159 28 68 708
Fragments arranged by size:
708 159 68 28
BbvCI CC'TCA_GC
Cuts at: 0 787 820 963
Size: 787 33 143
Fragments arranged by size:
787 143 33
BlpI GC'TnA_GC
Cuts at: 0 121 963
Size: 121 842
Bme1580I G_kGCm'C
Cuts at: 0 143 871 960 963
Size: 143 728 89 3
Fragments arranged by size:
728 143 89 3
BmrI ACTGGGnnnn_n'
Cuts at: 0 310 596 963
Size: 310 286 367
Fragments arranged by size:
367 310 286
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 55 909 963
Size: 55 854 54
Fragments arranged by size:
854 55 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 232 274 790 879 963
Size: 50 182 42 516 89 84
Fragments arranged by size:
516 182 89 84 50 42
Bpu10I CC'TnA_GC
Cuts at: 0 146 403 787 820 963
Size: 146 257 384 33 143
Fragments arranged by size:
384 257 146 143 33
BsaI GGTCTCn'nnnn_
Cuts at: 0 766 963
Size: 766 197
BsaAI yAC'GTr
Cuts at: 0 230 963
Size: 230 733
BsaHI Gr'CG_yC
Cuts at: 0 491 963
Size: 491 472
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 898 963
Size: 28 870 65
Fragments arranged by size:
870 65 28
BseYI C'CCAG_C
Cuts at: 0 348 508 726 745 947 951 963
Size: 348 160 218 19 202 4 12
Fragments arranged by size:
348 218 202 160 19 12 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 681 779 963
Size: 681 98 184
Fragments arranged by size:
681 184 98
BsiEI CG_ry'CG
Cuts at: 0 735 963
Size: 735 228
Bsp1286I G_dGCh'C
Cuts at: 0 36 62 143 871 960 963
Size: 36 26 81 728 89 3
Fragments arranged by size:
728 89 81 36 26 3
BsrDI GCAATG_nn'
Cuts at: 0 170 963
Size: 170 793
BsrFI r'CCGG_y
Cuts at: 0 8 518 963
Size: 8 510 445
Fragments arranged by size:
510 445 8
BssSI C'ACGA_G
Cuts at: 0 558 963
Size: 558 405
BstEII G'GTnAC_C
Cuts at: 0 318 813 963
Size: 318 495 150
Fragments arranged by size:
495 318 150
BtgI C'CryG_G
Cuts at: 0 16 332 637 701 963
Size: 16 316 305 64 262
Fragments arranged by size:
316 305 262 64 16
BtsI GCAGTG_nn'
Cuts at: 0 10 182 963
Size: 10 172 781
Fragments arranged by size:
781 172 10
DraIII CAC_nnn'GTG
Cuts at: 0 315 963
Size: 315 648
EaeI y'GGCC_r
Cuts at: 0 392 732 963
Size: 392 340 231
Fragments arranged by size:
392 340 231
EagI C'GGCC_G
Cuts at: 0 732 963
Size: 732 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 232 274 790 879 963
Size: 50 182 42 516 89 84
Fragments arranged by size:
516 182 89 84 50 42
EcoO109I rG'GnC_Cy
Cuts at: 0 21 110 139 140 305 867 868 888
Size: 21 89 29 1 165 562 1 20
Cuts at: 888 963
Size: 75
Fragments arranged by size:
562 165 89 75 29 21 20 1
1
HaeII r_GCGC'y
Cuts at: 0 454 963
Size: 454 509
KpnI G_GTAC'C
Cuts at: 0 78 963
Size: 78 885
MscI TGG'CCA
Cuts at: 0 394 963
Size: 394 569
MslI CAynn'nnrTG
Cuts at: 0 175 939 963
Size: 175 764 24
Fragments arranged by size:
764 175 24
MspA1I CmG'CkG
Cuts at: 0 18 99 334 480 783 951 963
Size: 18 81 235 146 303 168 12
Fragments arranged by size:
303 235 168 146 81 18 12
NaeI GCC'GGC
Cuts at: 0 10 963
Size: 10 953
NcoI C'CATG_G
Cuts at: 0 701 963
Size: 701 262
NgoMIV G'CCGG_C
Cuts at: 0 8 963
Size: 8 955
NotI GC'GGCC_GC
Cuts at: 0 732 963
Size: 732 231
PflMI CCAn_nnn'nTGG
Cuts at: 0 315 963
Size: 315 648
PfoI T'CCnGG_A
Cuts at: 0 238 290 963
Size: 238 52 673
Fragments arranged by size:
673 238 52
PmlI CAC'GTG
Cuts at: 0 230 963
Size: 230 733
PpuMI rG'GwC_Cy
Cuts at: 0 110 305 888 963
Size: 110 195 583 75
Fragments arranged by size:
583 195 110 75
PshAI GACnn'nnGTC
Cuts at: 0 259 303 963
Size: 259 44 660
Fragments arranged by size:
660 259 44
PspOMI G'GGCC_C
Cuts at: 0 139 867 963
Size: 139 728 96
Fragments arranged by size:
728 139 96
PstI C_TGCA'G
Cuts at: 0 461 743 782 963
Size: 461 282 39 181
Fragments arranged by size:
461 282 181 39
PvuII CAG'CTG
Cuts at: 0 783 951 963
Size: 783 168 12
Fragments arranged by size:
783 168 12
SacII CC_GC'GG
Cuts at: 0 19 335 963
Size: 19 316 628
Fragments arranged by size:
628 316 19
SanDI GG'GwC_CC
Cuts at: 0 110 305 963
Size: 110 195 658
Fragments arranged by size:
658 195 110
SfcI C'TryA_G
Cuts at: 0 151 457 739 778 862 963
Size: 151 306 282 39 84 101
Fragments arranged by size:
306 282 151 101 84 39
SmaI CCC'GGG
Cuts at: 0 137 190 963
Size: 137 53 773
Fragments arranged by size:
773 137 53
StuI AGG'CCT
Cuts at: 0 287 963
Size: 287 676
StyI C'CwwG_G
Cuts at: 0 445 701 963
Size: 445 256 262
Fragments arranged by size:
445 262 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 362 943 963
Size: 362 581 20
Fragments arranged by size:
581 362 20
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 935 963
Size: 935 28
XmaI C'CCGG_G
Cuts at: 0 135 188 963
Size: 135 53 775
Fragments arranged by size:
775 135 53
ZraI GAC'GTC
Cuts at: 0 492 963
Size: 492 471
Enzymes that do cut:
AatII Acc65I AflIII AleI AlwNI ApaI AvaI BanI
BanII BbsI BbvCI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI
Bsp1286I BsrDI BsrFI BssSI BstEII BtgI BtsI DraIII
EaeI EagI Eco57MI EcoO109I HaeII KpnI MscI MslI
MspA1I NaeI NcoI NgoMIV NotI PflMI PfoI PmlI
PpuMI PshAI PspOMI PstI PvuII SacII SanDI SfcI
SmaI StuI StyI TaqII XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI AclI AcuI AfeI AflII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglI
BglII BmgBI BmtI BpuEI BsaBI BsaWI BsaXI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI
BssHII BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EarI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI MfeI MluI MmeI NarI NdeI
NheI NruI NsiI NspI PacI PasI PciI PmeI
PpiI PsiI PspXI PsrI PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI
TstI Tth111I XbaI XhoI XmnI