Restriction map for
pF1KB8456
(Linear) (Six-base) MAPSORT of: KIBB8456.seq Check: 191 from: 1 to: 1455
KIBB8456.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 869 1037 1199 1455
Size: 245 624 168 162 256
Fragments arranged by size:
624 256 245 168 162
AleI CACnn'nnGTG
Cuts at: 0 1087 1455
Size: 1087 368
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 851 1455
Size: 851 604
AlwNI CAG_nnn'CTG
Cuts at: 0 342 446 1455
Size: 342 104 1009
Fragments arranged by size:
1009 342 104
ApaLI G'TGCA_C
Cuts at: 0 922 1455
Size: 922 533
ApoI r'AATT_y
Cuts at: 0 161 218 1455
Size: 161 57 1237
Fragments arranged by size:
1237 161 57
AvaI C'yCGr_G
Cuts at: 0 946 1455
Size: 946 509
BamHI G'GATC_C
Cuts at: 0 843 1455
Size: 843 612
BanI G'GyrC_C
Cuts at: 0 462 942 1455
Size: 462 480 513
Fragments arranged by size:
513 480 462
BbsI GAAGACnn'nnnn_
Cuts at: 0 308 1455
Size: 308 1147
BclI T'GATC_A
Cuts at: 0 1091 1455
Size: 1091 364
BglI GCCn_nnn'nGGC
Cuts at: 0 1414 1455
Size: 1414 41
Bme1580I G_kGCm'C
Cuts at: 0 604 926 947 1455
Size: 604 322 21 508
Fragments arranged by size:
604 508 322 21
BmgBI CAC'GTC
Cuts at: 0 508 1455
Size: 508 947
BmtI G_CTAG'C
Cuts at: 0 776 1455
Size: 776 679
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 918 1455
Size: 918 537
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 442 577 902 1455
Size: 248 194 135 325 553
Fragments arranged by size:
553 325 248 194 135
Bpu10I CC'TnA_GC
Cuts at: 0 379 1455
Size: 379 1076
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 637 1455
Size: 65 572 818
Fragments arranged by size:
818 572 65
BsaI GGTCTCn'nnnn_
Cuts at: 0 418 1455
Size: 418 1037
BsaAI yAC'GTr
Cuts at: 0 927 1455
Size: 927 528
BsaHI Gr'CG_yC
Cuts at: 0 4 1455
Size: 4 1451
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 314 344 1455
Size: 314 30 1111
Fragments arranged by size:
1111 314 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 640 643 894 1201 1455
Size: 64 576 3 251 307 254
Fragments arranged by size:
576 307 254 251 64 3
BseYI C'CCAG_C
Cuts at: 0 596 682 1023 1062 1455
Size: 596 86 341 39 393
Fragments arranged by size:
596 393 341 86 39
BsiHKAI G_wGCw'C
Cuts at: 0 926 1455
Size: 926 529
Bsp1286I G_dGCh'C
Cuts at: 0 604 926 947 1455
Size: 604 322 21 508
Fragments arranged by size:
604 508 322 21
BsrDI GCAATG_nn'
Cuts at: 0 364 669 845 1455
Size: 364 305 176 610
Fragments arranged by size:
610 364 305 176
BsrFI r'CCGG_y
Cuts at: 0 1336 1455
Size: 1336 119
BsrGI T'GTAC_A
Cuts at: 0 415 1455
Size: 415 1040
BstYI r'GATC_y
Cuts at: 0 843 1455
Size: 843 612
BtsI GCAGTG_nn'
Cuts at: 0 529 647 1046 1424 1455
Size: 529 118 399 378 31
Fragments arranged by size:
529 399 378 118 31
DrdI GACnn_nn'nnGTC
Cuts at: 0 704 1455
Size: 704 751
EaeI y'GGCC_r
Cuts at: 0 1421 1455
Size: 1421 34
EarI CTCTTCn'nnn_
Cuts at: 0 15 188 883 932 953 1142 1247 1262
Size: 15 173 695 49 21 189 105 15
Cuts at: 1262 1455
Size: 193
Fragments arranged by size:
695 193 189 173 105 49 21 15
15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 248 442 577 869 902 1037 1199
Size: 245 3 194 135 292 33 135 162
Cuts at: 1199 1455
Size: 256
Fragments arranged by size:
292 256 245 194 162 135 135 33
3
EcoO109I rG'GnC_Cy
Cuts at: 0 814 1455
Size: 814 641
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1027 1276 1455
Size: 1027 249 179
Fragments arranged by size:
1027 249 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 1303 1335 1357 1389 1455
Size: 187 32 1084 32 22 32 66
Fragments arranged by size:
1084 187 66 32 32 32 22
HindIII A'AGCT_T
Cuts at: 0 861 1455
Size: 861 594
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 1455
Size: 745 710
MscI TGG'CCA
Cuts at: 0 1423 1455
Size: 1423 32
MslI CAynn'nnrTG
Cuts at: 0 1087 1455
Size: 1087 368
MspA1I CmG'CkG
Cuts at: 0 443 1359 1455
Size: 443 916 96
Fragments arranged by size:
916 443 96
NheI G'CTAG_C
Cuts at: 0 772 1455
Size: 772 683
PflMI CCAn_nnn'nTGG
Cuts at: 0 446 737 1455
Size: 446 291 718
Fragments arranged by size:
718 446 291
PmlI CAC'GTG
Cuts at: 0 927 1455
Size: 927 528
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 889 921 1455
Size: 889 32 534
Fragments arranged by size:
889 534 32
PstI C_TGCA'G
Cuts at: 0 539 654 1455
Size: 539 115 801
Fragments arranged by size:
801 539 115
SapI GCTCTTCn'nnn_
Cuts at: 0 188 1262 1455
Size: 188 1074 193
Fragments arranged by size:
1074 193 188
SfcI C'TryA_G
Cuts at: 0 535 650 1455
Size: 535 115 805
Fragments arranged by size:
805 535 115
SgrAI Cr'CCGG_yG
Cuts at: 0 1336 1455
Size: 1336 119
SmlI C'TyrA_G
Cuts at: 0 80 616 1455
Size: 80 536 839
Fragments arranged by size:
839 536 80
StyI C'CwwG_G
Cuts at: 0 117 1455
Size: 117 1338
TatI w'GTAC_w
Cuts at: 0 415 575 1455
Size: 415 160 880
Fragments arranged by size:
880 415 160
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 314 346 1455
Size: 314 32 1109
Fragments arranged by size:
1109 314 32
Tth111I GACn'n_nGTC
Cuts at: 0 509 1455
Size: 509 946
XbaI T'CTAG_A
Cuts at: 0 1321 1455
Size: 1321 134
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1059 1416 1455
Size: 1059 357 39
Fragments arranged by size:
1059 357 39
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApaLI ApoI AvaI BamHI
BanI BbsI BclI BglI Bme1580I BmgBI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsiHKAI Bsp1286I BsrDI BsrFI BsrGI BstYI BtsI
DrdI EaeI EarI Eco57MI EcoO109I FalI Hin4I HindIII
MmeI MscI MslI MspA1I NheI PflMI PmlI PpiI
PstI SapI SfcI SgrAI SmlI StyI TatI TstI
Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaI AscI AseI AsiSI AvrII
BaeI BanII BbeI BbvCI BcgI BciVI BfrBI BglII
BlpI BmrI BsaBI BsaWI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII XhoI XmaI XmnI
ZraI