Restriction map for
pF1KB8457
(Linear) (Six-base) MAPSORT of: KIBB8457.seq Check: 9881 from: 1 to: 1173
KIBB8457.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 792 1173
Size: 792 381
AflIII A'CryG_T
Cuts at: 0 179 1173
Size: 179 994
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 343 375 460 492 1173
Size: 343 32 85 32 681
Fragments arranged by size:
681 343 85 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 763 784 826 892 959 1173
Size: 763 21 42 66 67 214
Fragments arranged by size:
763 214 67 66 42 21
ApaI G_GGCC'C
Cuts at: 0 611 886 1173
Size: 611 275 287
Fragments arranged by size:
611 287 275
ApoI r'AATT_y
Cuts at: 0 1135 1173
Size: 1135 38
AvaI C'yCGr_G
Cuts at: 0 124 553 926 1040 1173
Size: 124 429 373 114 133
Fragments arranged by size:
429 373 133 124 114
BanI G'GyrC_C
Cuts at: 0 25 279 1077 1173
Size: 25 254 798 96
Fragments arranged by size:
798 254 96 25
BanII G_rGCy'C
Cuts at: 0 554 611 886 1173
Size: 554 57 275 287
Fragments arranged by size:
554 287 275 57
BbeI G_GCGC'C
Cuts at: 0 29 283 1081 1173
Size: 29 254 798 92
Fragments arranged by size:
798 254 92 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 162 1173
Size: 162 1011
BbvCI CC'TCA_GC
Cuts at: 0 190 619 777 893 1148 1173
Size: 190 429 158 116 255 25
Fragments arranged by size:
429 255 190 158 116 25
BclI T'GATC_A
Cuts at: 0 110 1173
Size: 110 1063
BfrBI ATG'CAT
Cuts at: 0 147 1173
Size: 147 1026
BlpI GC'TnA_GC
Cuts at: 0 412 1173
Size: 412 761
Bme1580I G_kGCm'C
Cuts at: 0 611 886 1109 1173
Size: 611 275 223 64
Fragments arranged by size:
611 275 223 64
BmgBI CAC'GTC
Cuts at: 0 182 1173
Size: 182 991
BmrI ACTGGGnnnn_n'
Cuts at: 0 880 904 969 1096 1173
Size: 880 24 65 127 77
Fragments arranged by size:
880 127 77 65 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 656 1173
Size: 438 218 517
Fragments arranged by size:
517 438 218
Bpu10I CC'TnA_GC
Cuts at: 0 190 619 777 893 1148 1173
Size: 190 429 158 116 255 25
Fragments arranged by size:
429 255 190 158 116 25
BsaI GGTCTCn'nnnn_
Cuts at: 0 518 1173
Size: 518 655
BsaHI Gr'CG_yC
Cuts at: 0 26 280 1078 1173
Size: 26 254 798 95
Fragments arranged by size:
798 254 95 26
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 343 373 436 466 1173
Size: 343 30 63 30 707
Fragments arranged by size:
707 343 63 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 607 1165 1173
Size: 607 558 8
Fragments arranged by size:
607 558 8
BseYI C'CCAG_C
Cuts at: 0 65 368 693 735 756 819 874 971
Size: 65 303 325 42 21 63 55 97
Cuts at: 971 1108 1173
Size: 137 65
Fragments arranged by size:
325 303 137 97 65 65 63 55
42 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 465 1173
Size: 293 172 708
Fragments arranged by size:
708 293 172
BsiHKAI G_wGCw'C
Cuts at: 0 220 1173
Size: 220 953
Bsp1286I G_dGCh'C
Cuts at: 0 220 554 611 886 1109 1173
Size: 220 334 57 275 223 64
Fragments arranged by size:
334 275 223 220 64 57
BspHI T'CATG_A
Cuts at: 0 638 1173
Size: 638 535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 792 942 1173
Size: 792 150 231
Fragments arranged by size:
792 231 150
BsrBI CCG'CTC
Cuts at: 0 503 566 1017 1173
Size: 503 63 451 156
Fragments arranged by size:
503 451 156 63
BsrFI r'CCGG_y
Cuts at: 0 327 1173
Size: 327 846
BssSI C'ACGA_G
Cuts at: 0 397 1173
Size: 397 776
BstAPI GCAn_nnn'nTGC
Cuts at: 0 264 1173
Size: 264 909
BstXI CCAn_nnnn'nTGG
Cuts at: 0 42 150 912 1173
Size: 42 108 762 261
Fragments arranged by size:
762 261 108 42
BtgI C'CryG_G
Cuts at: 0 156 198 440 713 797 846 1173
Size: 156 42 242 273 84 49 327
Fragments arranged by size:
327 273 242 156 84 49 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 208 1173
Size: 208 965
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 183 218 1173
Size: 183 35 955
Fragments arranged by size:
955 183 35
EaeI y'GGCC_r
Cuts at: 0 245 329 447 981 1173
Size: 245 84 118 534 192
Fragments arranged by size:
534 245 192 118 84
EarI CTCTTCn'nnn_
Cuts at: 0 1061 1173
Size: 1061 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 124 577 1173
Size: 124 453 596
Fragments arranged by size:
596 453 124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 656 1173
Size: 438 218 517
Fragments arranged by size:
517 438 218
EcoO109I rG'GnC_Cy
Cuts at: 0 608 718 802 922 931 1097 1173
Size: 608 110 84 120 9 166 76
Fragments arranged by size:
608 166 120 110 84 76 9
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 432 1173
Size: 432 741
HaeII r_GCGC'y
Cuts at: 0 29 283 763 1081 1173
Size: 29 254 480 318 92
Fragments arranged by size:
480 318 254 92 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 207 239 285 317 436 468 886 918
Size: 207 32 46 32 119 32 418 32
Cuts at: 918 1173
Size: 255
Fragments arranged by size:
418 255 207 119 46 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 25 279 1077 1173
Size: 25 254 798 96
Fragments arranged by size:
798 254 96 25
MscI TGG'CCA
Cuts at: 0 247 1173
Size: 247 926
MslI CAynn'nnrTG
Cuts at: 0 148 1173
Size: 148 1025
MspA1I CmG'CkG
Cuts at: 0 261 664 682 874 1173
Size: 261 403 18 192 299
Fragments arranged by size:
403 299 261 192 18
NarI GG'CG_CC
Cuts at: 0 26 280 1078 1173
Size: 26 254 798 95
Fragments arranged by size:
798 254 95 26
NcoI C'CATG_G
Cuts at: 0 846 1173
Size: 846 327
NsiI A_TGCA'T
Cuts at: 0 149 1173
Size: 149 1024
NspI r_CATG'y
Cuts at: 0 151 1173
Size: 151 1022
PasI CC'CwG_GG
Cuts at: 0 12 1173
Size: 12 1161
PfoI T'CCnGG_A
Cuts at: 0 211 1173
Size: 211 962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 343 375 1173
Size: 343 32 798
Fragments arranged by size:
798 343 32
PpuMI rG'GwC_Cy
Cuts at: 0 718 802 922 931 1097 1173
Size: 718 84 120 9 166 76
Fragments arranged by size:
718 166 120 84 76 9
PspOMI G'GGCC_C
Cuts at: 0 607 882 1173
Size: 607 275 291
Fragments arranged by size:
607 291 275
PstI C_TGCA'G
Cuts at: 0 105 260 663 1173
Size: 105 155 403 510
Fragments arranged by size:
510 403 155 105
PvuII CAG'CTG
Cuts at: 0 261 664 874 1173
Size: 261 403 210 299
Fragments arranged by size:
403 299 261 210
SanDI GG'GwC_CC
Cuts at: 0 922 1173
Size: 922 251
SfcI C'TryA_G
Cuts at: 0 101 256 659 1173
Size: 101 155 403 514
Fragments arranged by size:
514 403 155 101
SfoI GGC'GCC
Cuts at: 0 27 281 1079 1173
Size: 27 254 798 94
Fragments arranged by size:
798 254 94 27
SmaI CCC'GGG
Cuts at: 0 555 1042 1173
Size: 555 487 131
Fragments arranged by size:
555 487 131
SrfI GCCC'GGGC
Cuts at: 0 555 1042 1173
Size: 555 487 131
Fragments arranged by size:
555 487 131
StyI C'CwwG_G
Cuts at: 0 603 846 1173
Size: 603 243 327
Fragments arranged by size:
603 327 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 57 134 235 536 1173
Size: 57 77 101 301 637
Fragments arranged by size:
637 301 101 77 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1054 1086 1173
Size: 1054 32 87
Fragments arranged by size:
1054 87 32
Tth111I GACn'n_nGTC
Cuts at: 0 854 1173
Size: 854 319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 39 462 1173
Size: 39 423 711
Fragments arranged by size:
711 423 39
XmaI C'CCGG_G
Cuts at: 0 553 1040 1173
Size: 553 487 133
Fragments arranged by size:
553 487 133
Enzymes that do cut:
AarI AflIII AloI AlwNI ApaI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BclI BfrBI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI
BssSI BstAPI BstXI BtgI BtgZI CspCI EaeI EarI
EciI Eco57MI EcoO109I FalI HaeII Hin4I KasI MscI
MslI MspA1I NarI NcoI NsiI NspI PasI PfoI
PpiI PpuMI PspOMI PstI PvuII SanDI SfcI SfoI
SmaI SrfI StyI TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BglI BglII BmtI BplI
BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BsrDI BsrGI BssHII BstBI BstEII BstYI BstZ17I
Bsu36I BtsI ClaI DraI DraIII DrdI EagI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI NaeI NdeI NgoMIV
NheI NotI NruI PacI PciI PflMI PmeI PmlI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SspI StuI SwaI TatI
XbaI XhoI XmnI ZraI