Restriction map for
pF1KB8458
(Linear) (Six-base) MAPSORT of: KIBB8458.seq Check: 1456 from: 1 to: 1017
KIBB8458.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 414 1017
Size: 414 603
AatII G_ACGT'C
Cuts at: 0 386 1017
Size: 386 631
AccI GT'mk_AC
Cuts at: 0 278 1001 1017
Size: 278 723 16
Fragments arranged by size:
723 278 16
AfeI AGC'GCT
Cuts at: 0 214 911 1017
Size: 214 697 106
Fragments arranged by size:
697 214 106
AlwNI CAG_nnn'CTG
Cuts at: 0 18 1017
Size: 18 999
ApoI r'AATT_y
Cuts at: 0 567 1017
Size: 567 450
BamHI G'GATC_C
Cuts at: 0 148 1017
Size: 148 869
BanI G'GyrC_C
Cuts at: 0 426 1017
Size: 426 591
BanII G_rGCy'C
Cuts at: 0 818 1017
Size: 818 199
BbsI GAAGACnn'nnnn_
Cuts at: 0 142 743 1017
Size: 142 601 274
Fragments arranged by size:
601 274 142
BbvCI CC'TCA_GC
Cuts at: 0 869 1017
Size: 869 148
BciVI GTATCCnnnnn_n'
Cuts at: 0 308 1017
Size: 308 709
BglI GCCn_nnn'nGGC
Cuts at: 0 67 1017
Size: 67 950
BglII A'GATC_T
Cuts at: 0 777 1017
Size: 777 240
BlpI GC'TnA_GC
Cuts at: 0 349 1017
Size: 349 668
Bme1580I G_kGCm'C
Cuts at: 0 243 431 631 1017
Size: 243 188 200 386
Fragments arranged by size:
386 243 200 188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 580 599 643 832 1017
Size: 226 354 19 44 189 185
Fragments arranged by size:
354 226 189 185 44 19
Bpu10I CC'TnA_GC
Cuts at: 0 869 1017
Size: 869 148
BsaAI yAC'GTr
Cuts at: 0 282 1017
Size: 282 735
BsaHI Gr'CG_yC
Cuts at: 0 383 1017
Size: 383 634
BsaWI w'CCGG_w
Cuts at: 0 48 386 1017
Size: 48 338 631
Fragments arranged by size:
631 338 48
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 223 586 709 1017
Size: 103 120 363 123 308
Fragments arranged by size:
363 308 123 120 103
BseYI C'CCAG_C
Cuts at: 0 11 267 1017
Size: 11 256 750
Fragments arranged by size:
750 256 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1017
Size: 955 62
BsiHKAI G_wGCw'C
Cuts at: 0 818 1017
Size: 818 199
Bsp1286I G_dGCh'C
Cuts at: 0 243 431 631 818 1017
Size: 243 188 200 187 199
Fragments arranged by size:
243 200 199 188 187
BspEI T'CCGG_A
Cuts at: 0 48 386 1017
Size: 48 338 631
Fragments arranged by size:
631 338 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 327 414 815 1017
Size: 327 87 401 202
Fragments arranged by size:
401 327 202 87
BsrDI GCAATG_nn'
Cuts at: 0 74 1017
Size: 74 943
BsrFI r'CCGG_y
Cuts at: 0 357 443 1017
Size: 357 86 574
Fragments arranged by size:
574 357 86
BssSI C'ACGA_G
Cuts at: 0 929 1017
Size: 929 88
BstYI r'GATC_y
Cuts at: 0 148 389 674 777 876 1017
Size: 148 241 285 103 99 141
Fragments arranged by size:
285 241 148 141 103 99
Bsu36I CC'TnA_GG
Cuts at: 0 692 1017
Size: 692 325
BtgI C'CryG_G
Cuts at: 0 644 1017
Size: 644 373
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 747 1017
Size: 747 270
BtsI GCAGTG_nn'
Cuts at: 0 243 1017
Size: 243 774
EaeI y'GGCC_r
Cuts at: 0 68 1017
Size: 68 949
EarI CTCTTCn'nnn_
Cuts at: 0 88 334 961 1017
Size: 88 246 627 56
Fragments arranged by size:
627 246 88 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 430 439 1017
Size: 430 9 578
Fragments arranged by size:
578 430 9
EcoICRI GAG'CTC
Cuts at: 0 816 1017
Size: 816 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 580 599 643 832 1017
Size: 226 354 19 44 189 185
Fragments arranged by size:
354 226 189 185 44 19
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 117 972 1017
Size: 117 855 45
Fragments arranged by size:
855 117 45
HaeII r_GCGC'y
Cuts at: 0 216 913 954 1017
Size: 216 697 41 63
Fragments arranged by size:
697 216 63 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 572 604 1017
Size: 572 32 413
Fragments arranged by size:
572 413 32
HincII GTy'rAC
Cuts at: 0 501 1017
Size: 501 516
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 875 1017
Size: 583 292 142
Fragments arranged by size:
583 292 142
MslI CAynn'nnrTG
Cuts at: 0 130 766 1017
Size: 130 636 251
Fragments arranged by size:
636 251 130
MspA1I CmG'CkG
Cuts at: 0 18 1017
Size: 18 999
NaeI GCC'GGC
Cuts at: 0 445 1017
Size: 445 572
NgoMIV G'CCGG_C
Cuts at: 0 443 1017
Size: 443 574
PflMI CCAn_nnn'nTGG
Cuts at: 0 713 1017
Size: 713 304
PvuII CAG'CTG
Cuts at: 0 18 1017
Size: 18 999
SacI G_AGCT'C
Cuts at: 0 818 1017
Size: 818 199
SapI GCTCTTCn'nnn_
Cuts at: 0 334 1017
Size: 334 683
SfcI C'TryA_G
Cuts at: 0 993 1017
Size: 993 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 274 354 1017
Size: 274 80 663
Fragments arranged by size:
663 274 80
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 279 1017
Size: 279 738
XmnI GAAnn'nnTTC
Cuts at: 0 44 1017
Size: 44 973
ZraI GAC'GTC
Cuts at: 0 384 1017
Size: 384 633
Enzymes that do cut:
AarI AatII AccI AfeI AlwNI ApoI BamHI BanI
BanII BbsI BbvCI BciVI BglI BglII BlpI Bme1580I
BpmI Bpu10I BsaAI BsaHI BsaWI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BspMI BsrDI BsrFI BssSI BstYI
Bsu36I BtgI BtgZI BtsI EaeI EarI EciI EcoICRI
Eco57MI FalI HaeII Hin4I HincII MmeI MslI MspA1I
NaeI NgoMIV PflMI PvuII SacI SapI SfcI TaqII
XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BcgI BclI BfrBI BmgBI BmrI
BmtI BplI BpuEI BsaI BsaBI BsaXI BsiEI BsiWI
BsmI BsmBI BspHI BsrBI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstZ17I ClaI CspCI DraI DraIII DrdI
EagI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MscI NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TatI TstI Tth111I XbaI XhoI
XmaI