Restriction map for
pF1KB8461
(Linear) (Six-base) MAPSORT of: KIBB8461.seq Check: 7508 from: 1 to: 576
KIBB8461.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AatII G_ACGT'C
Cuts at: 0 230 576
Size: 230 346
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 576
Size: 406 170
AlwNI CAG_nnn'CTG
Cuts at: 0 426 576
Size: 426 150
BanI G'GyrC_C
Cuts at: 0 305 340 576
Size: 305 35 236
Fragments arranged by size:
305 236 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 222 576
Size: 222 354
BbvCI CC'TCA_GC
Cuts at: 0 536 576
Size: 536 40
BglI GCCn_nnn'nGGC
Cuts at: 0 520 546 576
Size: 520 26 30
Fragments arranged by size:
520 30 26
BlpI GC'TnA_GC
Cuts at: 0 471 576
Size: 471 105
Bme1580I G_kGCm'C
Cuts at: 0 343 535 576
Size: 343 192 41
Fragments arranged by size:
343 192 41
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 465 576
Size: 465 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 576
Size: 484 92
Bpu10I CC'TnA_GC
Cuts at: 0 536 576
Size: 536 40
BsaHI Gr'CG_yC
Cuts at: 0 227 576
Size: 227 349
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 562 576
Size: 562 14
BseYI C'CCAG_C
Cuts at: 0 175 258 316 576
Size: 175 83 58 260
Fragments arranged by size:
260 175 83 58
BsiEI CG_ry'CG
Cuts at: 0 195 205 576
Size: 195 10 371
Fragments arranged by size:
371 195 10
BsiHKAI G_wGCw'C
Cuts at: 0 472 576
Size: 472 104
BsmBI CGTCTCn'nnnn_
Cuts at: 0 202 576
Size: 202 374
Bsp1286I G_dGCh'C
Cuts at: 0 343 472 535 576
Size: 343 129 63 41
Fragments arranged by size:
343 129 63 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 58 362 576
Size: 58 304 214
Fragments arranged by size:
304 214 58
BsrBI CCG'CTC
Cuts at: 0 207 576
Size: 207 369
BssHII G'CGCG_C
Cuts at: 0 558 576
Size: 558 18
BstAPI GCAn_nnn'nTGC
Cuts at: 0 50 576
Size: 50 526
BstXI CCAn_nnnn'nTGG
Cuts at: 0 132 333 576
Size: 132 201 243
Fragments arranged by size:
243 201 132
BtgI C'CryG_G
Cuts at: 0 38 576
Size: 38 538
BtsI GCAGTG_nn'
Cuts at: 0 170 309 576
Size: 170 139 267
Fragments arranged by size:
267 170 139
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 90 125 576
Size: 90 35 451
Fragments arranged by size:
451 90 35
EaeI y'GGCC_r
Cuts at: 0 202 434 576
Size: 202 232 142
Fragments arranged by size:
232 202 142
EagI C'GGCC_G
Cuts at: 0 202 576
Size: 202 374
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 484 576
Size: 406 78 92
Fragments arranged by size:
406 92 78
EcoO109I rG'GnC_Cy
Cuts at: 0 423 576
Size: 423 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 80 112 576
Size: 80 32 464
Fragments arranged by size:
464 80 32
MscI TGG'CCA
Cuts at: 0 436 576
Size: 436 140
MslI CAynn'nnrTG
Cuts at: 0 334 576
Size: 334 242
PfoI T'CCnGG_A
Cuts at: 0 84 576
Size: 84 492
PshAI GACnn'nnGTC
Cuts at: 0 226 576
Size: 226 350
PstI C_TGCA'G
Cuts at: 0 568 576
Size: 568 8
SfcI C'TryA_G
Cuts at: 0 564 576
Size: 564 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 520 576
Size: 520 56
StuI AGG'CCT
Cuts at: 0 491 576
Size: 491 85
StyI C'CwwG_G
Cuts at: 0 437 576
Size: 437 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 339 496 576
Size: 339 157 80
Fragments arranged by size:
339 157 80
TatI w'GTAC_w
Cuts at: 0 92 576
Size: 92 484
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 314 346 576
Size: 314 32 230
Fragments arranged by size:
314 230 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 15 132 333 576
Size: 15 117 201 243
Fragments arranged by size:
243 201 117 15
ZraI GAC'GTC
Cuts at: 0 228 576
Size: 228 348
Enzymes that do cut:
AatII AcuI AlwNI BanI BbsI BbvCI BglI BlpI
Bme1580I BplI BpmI Bpu10I BsaHI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BssHII BstAPI BstXI
BtgI BtsI CspCI EaeI EagI Eco57MI EcoO109I Hin4I
MscI MslI PfoI PshAI PstI SfcI SfiI StuI
StyI TaqII TatI TstI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanII BbeI
BcgI BciVI BclI BfrBI BglII BmgBI BmrI BmtI
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiWI
BsmI BspEI BspHI BsrDI BsrFI BsrGI BssSI BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI ClaI DraI DraIII
DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XhoI XmaI XmnI