Restriction map for
pF1KB8464
(Linear) (Six-base) MAPSORT of: KIBB8464.seq Check: 3999 from: 1 to: 2379
KIBB8464.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2163 2379
Size: 2163 216
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1667 1706 2074 2379
Size: 1667 39 368 305
Fragments arranged by size:
1667 368 305 39
AfeI AGC'GCT
Cuts at: 0 937 2379
Size: 937 1442
AleI CACnn'nnGTG
Cuts at: 0 1723 2379
Size: 1723 656
AlwNI CAG_nnn'CTG
Cuts at: 0 381 543 1213 2178 2379
Size: 381 162 670 965 201
Fragments arranged by size:
965 670 381 201 162
ApaI G_GGCC'C
Cuts at: 0 918 2379
Size: 918 1461
ApoI r'AATT_y
Cuts at: 0 196 751 2379
Size: 196 555 1628
Fragments arranged by size:
1628 555 196
AvaI C'yCGr_G
Cuts at: 0 707 2379
Size: 707 1672
BamHI G'GATC_C
Cuts at: 0 209 2379
Size: 209 2170
BanI G'GyrC_C
Cuts at: 0 24 533 1605 1884 1974 2130 2379
Size: 24 509 1072 279 90 156 249
Fragments arranged by size:
1072 509 279 249 156 90 24
BanII G_rGCy'C
Cuts at: 0 56 918 2039 2379
Size: 56 862 1121 340
Fragments arranged by size:
1121 862 340 56
BbeI G_GCGC'C
Cuts at: 0 537 2379
Size: 537 1842
BbsI GAAGACnn'nnnn_
Cuts at: 0 2062 2175 2379
Size: 2062 113 204
Fragments arranged by size:
2062 204 113
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1309 1343 2379
Size: 1309 34 1036
Fragments arranged by size:
1309 1036 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1517 2379
Size: 1517 862
BglII A'GATC_T
Cuts at: 0 650 2379
Size: 650 1729
BlpI GC'TnA_GC
Cuts at: 0 1409 2379
Size: 1409 970
Bme1580I G_kGCm'C
Cuts at: 0 29 47 428 918 1106 1730 1889 2135
Size: 29 18 381 490 188 624 159 246
Cuts at: 2135 2379
Size: 244
Fragments arranged by size:
624 490 381 246 244 188 159 29
18
BmrI ACTGGGnnnn_n'
Cuts at: 0 1256 2379
Size: 1256 1123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2058 2379
Size: 2058 321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 1133 1190 1202 1336 1549 1736 1973
Size: 122 1011 57 12 134 213 187 237
Cuts at: 1973 2379
Size: 406
Fragments arranged by size:
1011 406 237 213 187 134 122 57
12
Bpu10I CC'TnA_GC
Cuts at: 0 317 2379
Size: 317 2062
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 2338 2379
Size: 670 1668 41
Fragments arranged by size:
1668 670 41
BsaI GGTCTCn'nnnn_
Cuts at: 0 409 2214 2379
Size: 409 1805 165
Fragments arranged by size:
1805 409 165
BsaBI GATnn'nnATC
Cuts at: 0 1178 1226 2379
Size: 1178 48 1153
Fragments arranged by size:
1178 1153 48
BsaHI Gr'CG_yC
Cuts at: 0 534 2379
Size: 534 1845
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 967 997 2379
Size: 967 30 1382
Fragments arranged by size:
1382 967 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 125 428 799 919 1006 1033 1483 1486
Size: 125 303 371 120 87 27 450 3
Cuts at: 1486 2095 2098 2379
Size: 609 3 281
Fragments arranged by size:
609 450 371 303 281 125 120 87
27 3 3
BseYI C'CCAG_C
Cuts at: 0 371 548 909 1031 1535 1639 1699 1983
Size: 371 177 361 122 504 104 60 284
Cuts at: 1983 2379
Size: 396
Fragments arranged by size:
504 396 371 361 284 177 122 104
60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 83 2379
Size: 37 46 2296
Fragments arranged by size:
2296 46 37
BsiEI CG_ry'CG
Cuts at: 0 8 2379
Size: 8 2371
Bsp1286I G_dGCh'C
Cuts at: 0 29 47 56 428 918 1106 1730 1889
Size: 29 18 9 372 490 188 624 159
Cuts at: 1889 2039 2135 2379
Size: 150 96 244
Fragments arranged by size:
624 490 372 244 188 159 150 96
29 18 9
BspHI T'CATG_A
Cuts at: 0 2232 2379
Size: 2232 147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2163 2379
Size: 2163 216
BsrBI CCG'CTC
Cuts at: 0 801 1413 2379
Size: 801 612 966
Fragments arranged by size:
966 801 612
BsrDI GCAATG_nn'
Cuts at: 0 455 2379
Size: 455 1924
BsrFI r'CCGG_y
Cuts at: 0 3 2379
Size: 3 2376
BstAPI GCAn_nnn'nTGC
Cuts at: 0 24 381 1101 2379
Size: 24 357 720 1278
Fragments arranged by size:
1278 720 357 24
BstEII G'GTnAC_C
Cuts at: 0 1497 2379
Size: 1497 882
BstXI CCAn_nnnn'nTGG
Cuts at: 0 177 1602 2307 2379
Size: 177 1425 705 72
Fragments arranged by size:
1425 705 177 72
BstYI r'GATC_y
Cuts at: 0 209 650 1256 1475 2379
Size: 209 441 606 219 904
Fragments arranged by size:
904 606 441 219 209
BtgI C'CryG_G
Cuts at: 0 47 2024 2379
Size: 47 1977 355
Fragments arranged by size:
1977 355 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1367 2379
Size: 1367 1012
EaeI y'GGCC_r
Cuts at: 0 5 179 521 1509 2324 2379
Size: 5 174 342 988 815 55
Fragments arranged by size:
988 815 342 174 55 5
EagI C'GGCC_G
Cuts at: 0 5 2379
Size: 5 2374
EarI CTCTTCn'nnn_
Cuts at: 0 616 776 2379
Size: 616 160 1603
Fragments arranged by size:
1603 616 160
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 318 583 1030 2379
Size: 318 265 447 1349
Fragments arranged by size:
1349 447 318 265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 1133 1190 1202 1336 1549 1667 1706
Size: 122 1011 57 12 134 213 118 39
Cuts at: 1706 1736 1973 2074 2379
Size: 30 237 101 305
Fragments arranged by size:
1011 305 237 213 134 122 118 101
57 39 30 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 928 2379
Size: 928 1451
EcoO109I rG'GnC_Cy
Cuts at: 0 914 2379
Size: 914 1465
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 261 2379
Size: 261 2118
FspI TGC'GCA
Cuts at: 0 1288 2095 2379
Size: 1288 807 284
Fragments arranged by size:
1288 807 284
FspAI rTGC'GCAy
Cuts at: 0 1288 2379
Size: 1288 1091
HaeII r_GCGC'y
Cuts at: 0 537 939 2379
Size: 537 402 1440
Fragments arranged by size:
1440 537 402
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 454 486 967 999 1498 1530 2194 2226
Size: 454 32 481 32 499 32 664 32
Cuts at: 2226 2287 2319 2379
Size: 61 32 60
Fragments arranged by size:
664 499 481 454 61 60 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 159 2379
Size: 159 2220
KasI G'GCGC_C
Cuts at: 0 533 2379
Size: 533 1846
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 885 2324 2379
Size: 237 648 1439 55
Fragments arranged by size:
1439 648 237 55
MscI TGG'CCA
Cuts at: 0 181 523 1511 2326 2379
Size: 181 342 988 815 53
Fragments arranged by size:
988 815 342 181 53
MslI CAynn'nnrTG
Cuts at: 0 1723 1939 2221 2245 2379
Size: 1723 216 282 24 134
Fragments arranged by size:
1723 282 216 134 24
MspA1I CmG'CkG
Cuts at: 0 381 552 1035 1323 2379
Size: 381 171 483 288 1056
Fragments arranged by size:
1056 483 381 288 171
NaeI GCC'GGC
Cuts at: 0 5 2379
Size: 5 2374
NarI GG'CG_CC
Cuts at: 0 534 2379
Size: 534 1845
NcoI C'CATG_G
Cuts at: 0 2024 2379
Size: 2024 355
NgoMIV G'CCGG_C
Cuts at: 0 3 2379
Size: 3 2376
NspI r_CATG'y
Cuts at: 0 1287 2142 2250 2379
Size: 1287 855 108 129
Fragments arranged by size:
1287 855 129 108
PfoI T'CCnGG_A
Cuts at: 0 755 1565 2379
Size: 755 810 814
Fragments arranged by size:
814 810 755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 436 468 2071 2103 2379
Size: 436 32 1603 32 276
Fragments arranged by size:
1603 436 276 32 32
PspOMI G'GGCC_C
Cuts at: 0 914 2379
Size: 914 1465
PstI C_TGCA'G
Cuts at: 0 2256 2379
Size: 2256 123
PvuII CAG'CTG
Cuts at: 0 381 552 1035 2379
Size: 381 171 483 1344
Fragments arranged by size:
1344 483 381 171
SbfI CC_TGCA'GG
Cuts at: 0 2256 2379
Size: 2256 123
SexAI A'CCwGG_T
Cuts at: 0 1371 2204 2379
Size: 1371 833 175
Fragments arranged by size:
1371 833 175
SfcI C'TryA_G
Cuts at: 0 1763 2252 2269 2379
Size: 1763 489 17 110
Fragments arranged by size:
1763 489 110 17
SfoI GGC'GCC
Cuts at: 0 535 2379
Size: 535 1844
SmlI C'TyrA_G
Cuts at: 0 685 2353 2379
Size: 685 1668 26
Fragments arranged by size:
1668 685 26
SphI G_CATG'C
Cuts at: 0 2250 2379
Size: 2250 129
SspI AAT'ATT
Cuts at: 0 153 2379
Size: 153 2226
StuI AGG'CCT
Cuts at: 0 1601 2379
Size: 1601 778
StyI C'CwwG_G
Cuts at: 0 341 2024 2379
Size: 341 1683 355
Fragments arranged by size:
1683 355 341
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 385 678 1063 1137 1728 1752 2379
Size: 385 293 385 74 591 24 627
Fragments arranged by size:
627 591 385 385 293 74 24
TatI w'GTAC_w
Cuts at: 0 641 1053 2379
Size: 641 412 1326
Fragments arranged by size:
1326 641 412
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 712 744 2379
Size: 712 32 1635
Fragments arranged by size:
1635 712 32
Tth111I GACn'n_nGTC
Cuts at: 0 1156 2206 2379
Size: 1156 1050 173
Fragments arranged by size:
1156 1050 173
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1878 2379
Size: 1878 501
Enzymes that do cut:
AarI AcuI AfeI AleI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbsI BcgI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstXI
BstYI BtgI BtgZI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PfoI PpiI PspOMI PstI PvuII SbfI
SexAI SfcI SfoI SmlI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbvCI BciVI BclI BfrBI BmgBI BmtI BsaAI BsaWI
BsiHKAI BsiWI BsmI BsmBI BspEI BsrGI BssHII BssSI
BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII
DrdI EcoICRI EcoRI EcoRV FseI HindIII HpaI KpnI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SwaI XbaI XhoI XmaI XmnI ZraI