(Linear) (Six-base) MAPSORT of: KIBB8465.seq  Check: 5959  from: 1  to: 378



KIBB8465.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AccI GT'mk_AC

Cuts at:      0     182     378 
   Size:        182     196
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     250     378 
   Size:        250     128
 
AflIII A'CryG_T

Cuts at:      0      55     378 
   Size:         55     323
 
AgeI A'CCGG_T

Cuts at:      0      70     378 
   Size:         70     308
 
AlwNI CAG_nnn'CTG

Cuts at:      0     283     378 
   Size:        283      95
 
BglI GCCn_nnn'nGGC

Cuts at:      0     319     378 
   Size:        319      59
 
BmgBI CAC'GTC

Cuts at:      0     340     352     378 
   Size:        340      12      26

  Fragments arranged by size: 

                 340      26      12
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     378 
   Size:        193     185
 
Bpu10I CC'TnA_GC

Cuts at:      0     276     378 
   Size:        276     102
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     378 
   Size:        292      86
 
BsaWI w'CCGG_w

Cuts at:      0      70     378 
   Size:         70     308
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      55     378 
   Size:         55     323
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     168     378 
   Size:        168     210
 
BsrFI r'CCGG_y

Cuts at:      0      70     378 
   Size:         70     308
 
BstZ17I GTA'TAC

Cuts at:      0     183     378 
   Size:        183     195
 
BtgI C'CryG_G

Cuts at:      0     189     378 
   Size:        189     189
 
DraIII CAC_nnn'GTG

Cuts at:      0      51     378 
   Size:         51     327
 
EaeI y'GGCC_r

Cuts at:      0     156     285     320     378 
   Size:        156     129      35      58

  Fragments arranged by size: 

                 156     129      58      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     327     378 
   Size:        327      51
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     250     378 
   Size:        193      57     128

  Fragments arranged by size: 

                 193     128      57
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     155     378 
   Size:        155     223
 
MspA1I CmG'CkG

Cuts at:      0     171     378 
   Size:        171     207
 
NspI r_CATG'y

Cuts at:      0      59     228     378 
   Size:         59     169     150

  Fragments arranged by size: 

                 169     150      59
 
PciI A'CATG_T

Cuts at:      0      55     378 
   Size:         55     323
 
PvuII CAG'CTG

Cuts at:      0     171     378 
   Size:        171     207
 
ScaI AGT'ACT

Cuts at:      0      26     378 
   Size:         26     352
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     319     378 
   Size:        319      59
 
SgrAI Cr'CCGG_yG

Cuts at:      0      70     378 
   Size:         70     308
 
SmlI C'TyrA_G

Cuts at:      0     307     378 
   Size:        307      71
 
StyI C'CwwG_G

Cuts at:      0     270     378 
   Size:        270     108
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     255     378 
   Size:        255     123
 
TatI w'GTAC_w

Cuts at:      0      24     378 
   Size:         24     354
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AgeI    AlwNI     BglI    BmgBI     BpmI
   Bpu10I    BpuEI    BsaWI    BsmBI    BspMI    BsrFI  BstZ17I     BtgI
   DraIII     EaeI     EarI  Eco57MI     MmeI   MspA1I     NspI     PciI
    PvuII     ScaI     SfiI    SgrAI     SmlI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
     BmrI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I
    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfcI     SfoI     SgfI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI