Restriction map for
pF1KB8466
(Linear) (Six-base) MAPSORT of: KIBB8466.seq Check: 1175 from: 1 to: 1254
KIBB8466.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 577 1254
Size: 577 677
AccI GT'mk_AC
Cuts at: 0 149 242 1254
Size: 149 93 1012
Fragments arranged by size:
1012 149 93
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 1254
Size: 212 1042
AflIII A'CryG_T
Cuts at: 0 89 1254
Size: 89 1165
AleI CACnn'nnGTG
Cuts at: 0 729 1254
Size: 729 525
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 747 997 1254
Size: 747 250 257
Fragments arranged by size:
747 257 250
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 301 333 1254
Size: 301 32 921
Fragments arranged by size:
921 301 32
AlwNI CAG_nnn'CTG
Cuts at: 0 43 1254
Size: 43 1211
ApaI G_GGCC'C
Cuts at: 0 103 1031 1254
Size: 103 928 223
Fragments arranged by size:
928 223 103
AvaI C'yCGr_G
Cuts at: 0 543 586 856 1254
Size: 543 43 270 398
Fragments arranged by size:
543 398 270 43
BanI G'GyrC_C
Cuts at: 0 710 723 1141 1254
Size: 710 13 418 113
Fragments arranged by size:
710 418 113 13
BanII G_rGCy'C
Cuts at: 0 103 275 881 1019 1031 1254
Size: 103 172 606 138 12 223
Fragments arranged by size:
606 223 172 138 103 12
BbeI G_GCGC'C
Cuts at: 0 714 1254
Size: 714 540
BbsI GAAGACnn'nnnn_
Cuts at: 0 531 1045 1254
Size: 531 514 209
Fragments arranged by size:
531 514 209
BbvCI CC'TCA_GC
Cuts at: 0 510 1254
Size: 510 744
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 847 881 1254
Size: 847 34 373
Fragments arranged by size:
847 373 34
BglI GCCn_nnn'nGGC
Cuts at: 0 58 787 1254
Size: 58 729 467
Fragments arranged by size:
729 467 58
BlpI GC'TnA_GC
Cuts at: 0 702 1254
Size: 702 552
Bme1580I G_kGCm'C
Cuts at: 0 103 554 726 850 1031 1254
Size: 103 451 172 124 181 223
Fragments arranged by size:
451 223 181 172 124 103
BmrI ACTGGGnnnn_n'
Cuts at: 0 646 1226 1254
Size: 646 580 28
Fragments arranged by size:
646 580 28
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 69 1254
Size: 69 1185
Bpu10I CC'TnA_GC
Cuts at: 0 510 1254
Size: 510 744
BsaAI yAC'GTr
Cuts at: 0 822 1254
Size: 822 432
BsaBI GATnn'nnATC
Cuts at: 0 1240 1254
Size: 1240 14
BsaHI Gr'CG_yC
Cuts at: 0 711 1254
Size: 711 543
BsaWI w'CCGG_w
Cuts at: 0 471 1254
Size: 471 783
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 45 874 1060 1063 1072 1075 1078 1090
Size: 45 829 186 3 9 3 3 12
Cuts at: 1090 1254
Size: 164
Fragments arranged by size:
829 186 164 45 12 9 3 3
3
BseYI C'CCAG_C
Cuts at: 0 177 282 706 1165 1254
Size: 177 105 424 459 89
Fragments arranged by size:
459 424 177 105 89
BsiEI CG_ry'CG
Cuts at: 0 632 1116 1162 1254
Size: 632 484 46 92
Fragments arranged by size:
632 484 92 46
BsiHKAI G_wGCw'C
Cuts at: 0 275 568 803 827 1254
Size: 275 293 235 24 427
Fragments arranged by size:
427 293 275 235 24
BsmI GAATG_Cn'
Cuts at: 0 54 1254
Size: 54 1200
Bsp1286I G_dGCh'C
Cuts at: 0 103 275 554 568 726 803 827 850
Size: 103 172 279 14 158 77 24 23
Cuts at: 850 881 1019 1031 1254
Size: 31 138 12 223
Fragments arranged by size:
279 223 172 158 138 103 77 31
24 23 14 12
BspHI T'CATG_A
Cuts at: 0 407 1254
Size: 407 847
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 577 686 1254
Size: 577 109 568
Fragments arranged by size:
577 568 109
BsrBI CCG'CTC
Cuts at: 0 449 1158 1254
Size: 449 709 96
Fragments arranged by size:
709 449 96
BsrFI r'CCGG_y
Cuts at: 0 591 610 1254
Size: 591 19 644
Fragments arranged by size:
644 591 19
BssSI C'ACGA_G
Cuts at: 0 729 1254
Size: 729 525
BstAPI GCAn_nnn'nTGC
Cuts at: 0 395 1254
Size: 395 859
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1173 1254
Size: 1173 81
BtgI C'CryG_G
Cuts at: 0 431 515 530 555 782 1254
Size: 431 84 15 25 227 472
Fragments arranged by size:
472 431 227 84 25 15
BtsI GCAGTG_nn'
Cuts at: 0 586 850 1254
Size: 586 264 404
Fragments arranged by size:
586 404 264
EaeI y'GGCC_r
Cuts at: 0 119 218 263 299 428 629 779 1113
Size: 119 99 45 36 129 201 150 334
Cuts at: 1113 1159 1175 1254
Size: 46 16 79
Fragments arranged by size:
334 201 150 129 119 99 79 46
45 36 16
EagI C'GGCC_G
Cuts at: 0 629 1113 1159 1254
Size: 629 484 46 95
Fragments arranged by size:
629 484 95 46
EarI CTCTTCn'nnn_
Cuts at: 0 280 799 1254
Size: 280 519 455
Fragments arranged by size:
519 455 280
EcoICRI GAG'CTC
Cuts at: 0 273 1254
Size: 273 981
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 1254
Size: 212 1042
EcoO109I rG'GnC_Cy
Cuts at: 0 978 1027 1028 1254
Size: 978 49 1 226
Fragments arranged by size:
978 226 49 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 984 1254
Size: 984 270
FspI TGC'GCA
Cuts at: 0 1006 1240 1254
Size: 1006 234 14
Fragments arranged by size:
1006 234 14
FspAI rTGC'GCAy
Cuts at: 0 1240 1254
Size: 1240 14
HaeII r_GCGC'y
Cuts at: 0 262 714 750 793 901 1254
Size: 262 452 36 43 108 353
Fragments arranged by size:
452 353 262 108 43 36
HincII GTy'rAC
Cuts at: 0 837 1254
Size: 837 417
KasI G'GCGC_C
Cuts at: 0 710 1254
Size: 710 544
MscI TGG'CCA
Cuts at: 0 1177 1254
Size: 1177 77
MslI CAynn'nnrTG
Cuts at: 0 199 361 412 688 729 769 775 1254
Size: 199 162 51 276 41 40 6 479
Fragments arranged by size:
479 276 199 162 51 41 40 6
MspA1I CmG'CkG
Cuts at: 0 458 557 654 706 738 917 1148 1254
Size: 458 99 97 52 32 179 231 106
Fragments arranged by size:
458 231 179 106 99 97 52 32
NaeI GCC'GGC
Cuts at: 0 593 612 1254
Size: 593 19 642
Fragments arranged by size:
642 593 19
NarI GG'CG_CC
Cuts at: 0 711 1254
Size: 711 543
NgoMIV G'CCGG_C
Cuts at: 0 591 610 1254
Size: 591 19 644
Fragments arranged by size:
644 591 19
NotI GC'GGCC_GC
Cuts at: 0 1159 1254
Size: 1159 95
NspI r_CATG'y
Cuts at: 0 9 579 1254
Size: 9 570 675
Fragments arranged by size:
675 570 9
PasI CC'CwG_GG
Cuts at: 0 81 1254
Size: 81 1173
PpuMI rG'GwC_Cy
Cuts at: 0 978 1254
Size: 978 276
PspOMI G'GGCC_C
Cuts at: 0 99 1027 1254
Size: 99 928 227
Fragments arranged by size:
928 227 99
PvuII CAG'CTG
Cuts at: 0 706 917 1254
Size: 706 211 337
Fragments arranged by size:
706 337 211
SacI G_AGCT'C
Cuts at: 0 275 1254
Size: 275 979
SacII CC_GC'GG
Cuts at: 0 558 1254
Size: 558 696
SapI GCTCTTCn'nnn_
Cuts at: 0 280 799 1254
Size: 280 519 455
Fragments arranged by size:
519 455 280
SexAI A'CCwGG_T
Cuts at: 0 152 1254
Size: 152 1102
SfoI GGC'GCC
Cuts at: 0 712 1254
Size: 712 542
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 492 1254
Size: 492 762
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AloI AlwNI
ApaI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BglI BlpI Bme1580I BmrI BplI Bpu10I BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrFI BssSI BstAPI BstXI BtgI
BtsI EaeI EagI EarI EcoICRI Eco57MI EcoO109I FalI
FspI FspAI HaeII HincII KasI MscI MslI MspA1I
NaeI NarI NgoMIV NotI NspI PasI PpuMI PspOMI
PvuII SacI SacII SapI SexAI SfoI TaqII
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI
BclI BfrBI BglII BmgBI BmtI BpmI BpuEI BsaI
BsaXI BsgI BsiWI BsmBI BspEI BsrDI BsrGI BssHII
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EciI EcoNI EcoRI EcoRV FseI
Hin4I HindIII HpaI KpnI MfeI MluI MmeI NcoI
NdeI NheI NruI NsiI PacI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SalI SanDI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI